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Methi Lutein prevents Oxidative Stress induced DNA Damage and Cytotoxicity

Author Affiliations

  • 1Adichunchanagiri Bitotechnology and Cancer Research Institute, B.G.Nagara-571448, Mandya, Karnataka, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (2), Pages 54-61, February,10 (2015)


The detrimental effect of oxidative stress has been known since a long, which may cause the deleterious effect on DNA and finally leads to death. The present investigation revealed the DNA protectant effect of Lutein anti-oxidant purified from fresh Methi (Trigonellafoenumgraecum) leaves. Ferrous sulphate and ascorbic acid (10:100µM) induced the lipid peroxidation and fragmentation in DNA in human lymphocytes model systems. Methi lutein inhibits the formation of lipid peroxidation and DNA fragmentation in fresh human lymphocytes to the magnitude of 80% and 76% respectively. On treatment of Cow dung smoke condensate with human lymphocytes could lead to the adduct formation in human DNA which was controlled by the Methi Lutein (20M) effectively. U.V radiation induced DNA double strand break and cross linking was strongly controlled by the Methi lutein in Calf thymus DNA when compared to the standard antioxidants BHA and -tocopherol. Methi lutein might also avoid the cytotoxicity induced by the H2 and Fe:AA in fresh lymphocytes by maintaining the 80% viability even after 2 hours of treatment.


  1. Karanika S., Karantanos, T., Li L., Corn PG and Thompson T.C., DNA damage response and prostate cancer: defects, regulation and therapeutic implications, Oncogene.,10, 1038-238 (2014)
  2. In Young Kim., Yu-Ying He., Trehalose facilitates DNA melting : A single-molecule optical tweezers study, J Genes., 22, 1-11 (2014)
  3. Dizdaroglu M. and Simic M.G., (b) Radiation induced formation of thymine-thymine cross links, Int. J. Radiant Biol Relat Stud Phys Chem Med.,46, 241-246 (1984)
  4. Ensminger M., Iloff L., ebel C., Nikolova T., Kaina B. and Lobrich M., DNA breaks and chromosomal aberrations arise when replication meets base excision repair, J Cell Biol.,7, 206(1), 29-43 (2014)
  5. McMahon C.K. and T Soukalas S.N., In: Carcinogenesis: Polynuclear Aromatic hydrocarbons (Jones PW and Frudenthal RL eds) Raven Press New York., 61 (1976)
  6. Slupphaug G., Kavli B. and Krokan H.E., The interacting pathways for prevention and repair of oxidative DNA damage, Mutat. Res.,29,531, 231- 51(2003)
  7. Cooke M.S., Evans M.D., Dizdaroglu M., and Lunec J., Oxidative DNA damage : Mechanisms, mutation, and disease, FASEB J.,17, 1195-1214 (2003)
  8. Valko M., Morris H. and Cronin. MTD., Metals, Toxicity and Oxidative Stress, Current Med. Chem., 12, 1161-1208 (2005)
  9. Parthasarathy S., Santanam N., Ramachandran S. and Meilhac O., Oxidants and antioxidants in atherogenesis : An appraisal, Journal of Lipid Research, 40, 2143-157 (1999)
  10. Frei B., Reactive oxygen species and antioxidant vitamins, Linus Pauling Institute, Oregon State University., (1997)
  11. Singh R.P., Sharad S. and Kapur S., Free radicals and oxidative stress in neurodegenerative diseases: Relevance of Dietary Antioxidants, JIACM., 218-25 (2004)
  12. Valko M., Izakovic M., Mazur M., Rhodes C.J. and Telser J., Role of oxygen radicals in DNA damage and cancer incidence, Mol and Cell Biochem.,26, 637-56 (2004)
  13. Valko M., Leibfritz D., Moncola J., Cronin MTD., Mazura M. and Telser J., Free radicals and antioxidants in normal physiological functions and human disease, The Int J Biochem and Cell Biol., 39, 44-84 (2007)
  14. Hai Chen., Hideyuki Yoshioka., Gab Seok Kim., JooEun Jung., NobuyaOkami., Hiroyuki Sakata., Carolina M., Maier., PurnimaNarasimhan., Christina E.. Goeders and Pak H. Chan.,Oxidative Stress in Ischemic Brain Damage : Mechanisms of Cell Death and Potential Molecular Targets for Neuroprotection, Antioxidants and Redox signaling., 14(8), 1505-1517 (2011)
  15. Halliwell B., Antioxidants in human health and disease. Annual Review of Nutrition.,16, 33-50 (1996)
  16. Liu S., Lee I.M., Ajani U., Cole S.R., Buring J.E. and Manson J.E., Intake of vegetables rich in carotenoids and risk of coronary heart disease in men : The Physicians’ Health Study, Int J Epidemiol., 30, 130–35 (2001)
  17. Greenberg E.R. and Sporn M.B., Antioxidant vitamins, cancer and cardiovascular disease, N Engl J Med.,334, 1189–90 (1996)
  18. Hung H.C., Joshipura K.J. and Jiang R., Fruit and vegetable intake and risk of major chronic disease, J Natl Cancer Ins.,96, 1577–84 (2004)
  19. Gate L., Paul J., Ba G.N., Tew K.D. and Tapiero H., Oxidative stress induced in pathologies : The role of antioxidants, Biomed Pharmacother.,53, 169–180 (1999)
  20. Raghavendra P.R., Thammanna Gowda S.S. and Leela Srinivas, Smoke treatment triggers the release of a novel DNA damaging factor by lymphocytes, J Mol. Cellular Biochem.,12(284) 73-79 (2006)
  21. Shalini V.K. and Srinivas L., Fuel smoke condensate induced DNA damage in human lymphocytes and protection by Turmeric (Curcuma longa), Mol. Cel Beioch., 95, 21-30 (1990)
  22. Phillips H.J., Dye exclusion tests for cell viability. In. Tissue culture methods (Kruse PF, Patterson MK eds)., 406-408 (1973)
  23. Burton K., A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid, Biochem. J., 62, 315 (1956)
  24. Nakayama T., Kaneko M. and Nagata C., Cigarette induces DNA strand breaks in human cells, Nature.,314, 462-464 (1985)
  25. Shalini V.K., Luthra M., Srinivas L., Rao H., Basti S., Reddy M. and Balasubramanian D., Oxidative damage to the eye lens caused by cigarette smoke and fuel smoke condensate, Ind J Biochem and Biophys.,31, 261-266 (1994)
  26. Vandana S Poovala., Vijaya K., Kanji, Hiroyasu tachikawa. and Abdulla K Salahudeen., Role of oxidant stress and Antioxidant protection in acephate-induced Renal Tubular cytotoxicity, Tox Sci.,46, 403-409 (1998)
  27. Ashraf Shabani. and Azra Rabbani., Lead nitrate induced apoptosis in alveolar macrophages from rat lung, Toicology.,149, 109-114 (2000)