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Characterization of Pseudomonas savastoni pv. phaseolicola in Seed lots of Mung bean (Vigna radiata L. Wilczek ) collected from Rajasthan, India

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Author Affiliations

  • 1Department of Botany, University of Rajasthan, Jaipur, 302004, Rajasthan, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (10), Pages 57-61, October,10 (2015)

Abstract

Halo blight caused by Pseudomonas savastoni pv. phaseolicola (Pph) of mungbean is an important seed-borne bacterialdisease accounting for a heavy loss in its production worldwide. In the present study, a total of 151 seeds were collectedfrom fields of 14 districts of Rajasthan state and screened for occurrence and percent incidence of Pph. The pathogen wasisolated from infected and healthy looking seeds. A total of 30 isolates were found and identified by growth on a semiselective agar medium MSP, LOPAT tests and biochemical characteristics such as sugar utilization, gelatin liquefaction,H2S production and esculin hydrolysis etc. medium MSP was used for isolation and confirmation of the pathogen. The higherincidence of Pph on MSP was recorded in seed samples of Ajmer (50- 93%), Bhilwara (42- 71%), Churu (35-69%),Ganganagar (48-59%), Jalore(54-79%),Jhunjhunu(37-71%) and Nagaur(43-88%) districts.

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