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Preliminary Studies into the Efficacy of Different Brands of Toothpastes on the Oral Micro-Flora Before and after Brushing within Bingham University, Karu, Nasarawa State, Nigeria

Author Affiliations

  • 1Department of Biological Sciences, Bingham University, Karu, Nasarawa State, NIGERIA

Int. Res. J. Biological Sci., Volume 4, Issue (10), Pages 30-35, October,10 (2015)


This study was carried out to determine the efficacy of different brands of toothpaste against oral micro-flora before and after brushing within Bingham University. The method involved using a swab stick aseptically to collect samples before and after the use of toothpastes. The results from this study showed that five organisms were isolated from the oral cavity of Bingham University students before the use of the toothpastes, which includes: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus pyogenes and Candida albicans. In comparism with the toothpastes, TOO3 (Sensodyne) was the most effective against Staphylococcus aureus (60%), TOO1(Macleans) was the most effective against Staphylococcus epidermidis (51.7%), TOO5 (Darbur) was the most effective against Streptococcus mutans (60.6%) and Streptococcus pyogenes (60.4%), TOO4 was the most effective against Candida albicans (51.2%). TOO1 (Macleans) was the least effective against S. aureus (36.4%), S. mutans (26.4%), S. pyogenes (15.2%) and C. albicans (14.3%) while TOO2 (Himalaya) was the least effective against S. epidermidis (30.9%). The colony count of organisms showed a significant reduction in the microbial load of the oral cavity of the study sample size after use of the toothpaste. T-calculated = 5.612, T-tabulated (P &


  1. Okpalugo J., Ibrahim K. and Inyang U.S., Toothpaste formulation efficacy in reducing oral flora, Trop. J. of Pharm. Res., 8(1), 71-77 (2009)
  2. Wistreich G. and Lechtman M, Microbiology, fifth edition. Collier Macmillan, New York, 671-698 (1988)
  3. Hoikhian C.S.O. and Okoror L.O, Resistance of oral bacterial species to varied toothpaste effects, Int. J. of Eng. Res. and Sci. and Tech., 1(1), 1-10 (2012)
  4. Nwankwo I.U. and Ihesiulo S.C., Comparative Analysis of the Antibacterial Potentials of Some Brands of Toothpaste Commonly Used In Umuahia, Abia State. J. of Pharm. and Bio. Sci. (IOSR-JPBS), 9(3), 50-54 (2014)
  5. Panuti C.M, Mattos J.P, Ranoya P.N, Jesus A.M, Lotufo R.F and Romito G.A., Clinical effects of herbal dentifrice on the control of plague and gingivitis, A double blind study, Pesq. Olo. Bra. J., 17(4), 314 -318 (2003)
  6. Itthagarum A.K and Wei S.H., Analysis of fluoride ion concentration and in–vitrofluoride uptake from different commercial dentifrices, Int. dent. J., 46(4), 327-361 (1996)
  7. Degiam Z.D., An in Vitro Antimicrobial activity of six commercial toothpastes, This-qar. Med. J. (TQMJ), 4(4), 127-133 (2010)
  8. Manupati P, Antimicrobial Efficacy of Different Toothpastes and Mouth rinses: An in vitro Study. Dent, Res. J. (Isfahan), 8(2), 85–94 (2011)
  9. Jia-Xu Chen, Yue-Yun Liu, Shao-Xian Wang and Xiao-Hong Li, Efficacy of Crest Herbal Toothpaste in Clearing Internal Heat: A Randomized, Double-Blind Clinical Study, Evid. Bas. Comp. and Alt. med. J., (2013)
  10. William M.I and Cummuns D.M., The Technology behind close-up total advanced fresh, comprehensive continue education on dentals, Brit. J. of Dent. Surg., 24(5), 4-9 (2003)
  11. Mohan Kumar KP, Priya NK and Madhushankari GS., Anti-Cariogenic Efficacy of Herbal and Conventional Toothpastes - A Comparative In-Vitro Study, J. of Int. Oral Health, 5(2), 8-13 (2013)
  12. Akwa V.L., Bimbol A.L., Samaila K.L. and Marcus N.D., Geography perspective Of Nasarawa state, Native printing and publishing company Ltd, Keffi, Nasarawa State, Nigeria (2007)
  13. Obiekezie S.O., Odu N.N., Onwumene R. and Iloegbunam R.O., Bacteriological quality assessment of ready to eat salad sold within Bingham University, Karu, Nasarawa state, Nigeria, J. of Nat. and App. Sci., 1(2), 137-142 (2012)
  14. Ochei J.O. and Kolhatkar A.A., Medical Laboratory Science Theory and Practice sixth edition, Tata Mc Graw-Hill Publishing Company Limited, New Delhi, 644-657 (2007)
  15. Cheesbrough M., District Laboratory practice in tropical countries Part 2 Second edition, Cambridge University Press, New York, 157-224 (2006)
  16. Gamboa F.C., Estupinan M.K and Galindo A.O., Presence of Streptococcus mutans is saliva and its relationship with dental caries, Antimicrobial Susceptibility of the Isolates, Univ. Sci. J., 9(2), 23-27 (2004)
  17. Lee S.S., Zhang W. and Li Y., The antimicrobial potential of 14 natural herbal dentifrices: results of anin vitro diffusion method study, J. of the Amer. Dent. Assoc., 135(8), 1133-1141 (2004)
  18. Marsh P.D. and Martin M.V., Oral Microbiology fifth edition, Churchill Livingstone, London, 232 (2009)
  19. Larone D.H., Identification of fungi in culture, In: A guide to identification of medically important fungi fourth Edition, ASM Press, Washington DC; 229-253 (2002)
  20. Marinho V.C, Higgins J.P, Logan S.V, Sheiham A.L and Logan S.G., One Tropical fluoride (toothpaste, mouth rinses, gels or varnishes) versus another for preventing dental caries in children and adolescents, Cochrane Database System Review, 8(1), 2781 (2007)