Physiological Studies on the Monascus ruber Red Pigment and GC/MS Analysis
- 1Botany and Microbiology Department, Faculty of Science, Assiut University, PO Box 71516, Assiut, EGYPT
Int. Res. J. Biological Sci., Volume 4, Issue (10), Pages 1-14, October,10 (2015)
Physiological studies and effect of mono-sodium-glutamate (Na-G) and agitation on the two M. ruber strains are studied. The GC/MS analysis recorded that the different effects of Na-G and agitation on the red pigment, spectrophotometeric colour intensity, number and kinds of the antioxidant, flavouring metabolites. Visual estimation of the soluble pigment by tested M. ruber strains AUMC 4066 and 5705 were cultivated on four kinds of media including glucose, malt, malt+Na–glutamate and glucose+Na–glutamate, incubated statically and submerged at 120 rpm for 10 days at 30°C are studied. Also the colour intensity estimated at A 500nm by spectrophotometer are studied. The results clearing that the deepest red colour of the soluble pigment are recorded in malt, and malt+Na–glutamate of the tested of M. ruber strain AUMC 4066. The media fortified by glutamate are inhibited the red soluble colours and gives the red or brown-red colour. The highest colour intensity of the soluble pigment: are detected in submerged malt and malt+Na–glutamate of M. ruber 4066 are 3.528 and 3.477. Also all the reading clearing that the strain No.4066 have the highest colour intensity on the all tested media. Numbers and kinds of the metabolites detected in the ethanolic extracts of the selected strain AUMC 4066 has been studied by using GC/MS analysis. 140 flavouring and antioxidant metabolites are detected in the extracts of the four tested culture media incubated at 120rpm, 30°C for ten days. Malt medium recorded the highest numbers of metabolites followed by Malt+Na-glutamate, glucose+Na-glutamate and glucose media are 76, 25, 20 and 11, respectively. The highest numbers of the classified recorded metabolic products includes 48 phenolic compounds, 38 esters, 23 alkanes and alkenes, 10 ketons, 5 indolic compounds, 4 from (azole and terpenoids), 2 for each of (amides and amines), one for each of (aldehyde, free fatty acids, pyran and cyclo-arabitol) are detected at 1 mass fractions in the ethanolic extracts of the tested medium.
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