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Evaluation of Antimicrobial, Antioxidant and Cytotoxic Property of Pleurotus ostreatus Mushroom

Author Affiliations

  • 1Department of Pharmacy, Southeast University, Banani, 1213, Dhaka, BANGALADESH
  • 2National Mushroom Development and Extension Center, Sobhanbag, Savar, Dhaka, BANGALADESH

Int. Res. J. Biological Sci., Volume 4, Issue (1), Pages 29-33, January,10 (2015)


The study was conducted to estimate the performance of certain biological properties of oyster mushrooms (Pleurotus ostreatus). For determination of antimicrobial and cytotoxic activity of this mushroom disk diffusion technique and brine shrimp lethality bioassay were used. Ethyl acetate, hexane and chloroform extract were examined with ten test organism in which hexane extract showed higher antibacterial activity than chloroform and ethyl acetate for both gram positive and negative bacteria. The average zone of inhibition for hexane extract was in the range of 11-24mm. The total phenolic content ranged from 3.69 mg GAE/gm to 8.49 mg GAE/gm. In brine shrimp lethality bioassay LC50 values obtained were 10 ΅g/ml, 40 ΅g/ml and 75΅g/ml for hexane, ethyl acetate and chloroform, respectively. This study revealed that P. ostreatus mushroom has potential antimicrobial effect with significant antioxidant and anticancer activity.


  1. Soumya C, Gunjan B, Saikat K.B. and Krishnendu A., Antineoplastic effect of mushrooms, Australian Journal of Crop Science, 5, 904-911 (2011)
  2. S. Monira., A. Haque., A. Muhit., N. C. Sarker., A.H.M.K. Alam., A.A. Rahman. and P. Khondkal., Antimicrobial, antioxidant and cytotoxic properties of hypsizygus tessulatus cultivated in Bangladesh, Research Journal of Medicinal Plant., 6, 300-308 (2012)
  3. Asaduzzaman K., Liakot A.K., Shahdat H., Mousumi Tania. and Nazim U., Investigation on the nutritional composition of common edible and medicinal mushrooms cultivated in Bangladesh, Bangladesh J Mushroom., 3, 21-28 (2009)
  4. Anderson E.E. and Ward C.R., The food value of mushroom agaricus campestris, Pool Am Soc Hort.,41, 2301-23023 (1999)
  5. Bilal Ahmad Wani., Bodha R.H. and Wani A.H., Nutritional and medicinal importance of mushroomsJournal of Medicinal Plants Research., 4, 2598-2604(2010)
  6. Solomon P Wasser, Current findings, future trends, and unsolved problems in studies of medicinal mushrooms, Appl Microbiol Biotechnol., DOI 10.1007/s00253-010-3067-4, (2010)
  7. Zaidman B.Z, Yassin M, Mahajna J and Wasser S.P., Medicinal mushroom modulators of molecular targets as cancer therapeutics, Appl Microbiol Biotechnol,67, 453-68 (2005)
  8. Song Wei and Leo J.L.D.V.G., Pro-and antioxidative properties of medicinal mushroom extracts, International Journal of Medicinal Mushrooms.,10, 315–324 (2008)
  9. Viktor B., Tatjana B., Vitaliy K., Tatjana B. and Vitaliy k., The influence of culinary-medicinal mushrooms: Agaricus bisporus, lentinula eodes and pleurotus ostreatus on injuries of gastric mucosa in rats evoked by stress. Proceedings of the 7thInternational Conference on Mushroom Biology and Mushroom Products (ICMBMP7), 306-311 (2011)
  10. Yashvant P., Ram N. and Singh V.K., Medicinal properties of pleurotus species (Oyster Mushroom): A review, World Journal of Fungal and Plant Biology., 3, 01-12 (2012)
  11. Kiran N., Meenakshi., Mukesh K. and Ajay Y., Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom).International Science Congress Association 33International Journal of Microbial Resource Technology.,1, 391-399 (2012)
  12. Pornariya C. and Kanok O.I., Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju. Science Asia.,35, 326–331 (2009)
  13. Mehmet A., Ayse N.O, Pnar E. and Sevda K., Antimicrobial activity of some edible mushrooms in the eastern and southeast anatolia region of turkey, Gazi University Journal of Science.,23, 125-130 (2010)
  14. Wolff E.R.S., Wisbeck E., Silveira M.L.L., Gern R.M.M., Pinho M.S.L. and Furlan S.A., Antimicrobial and antineoplasic activity of Pleurotus ostreatus,Appl Biochem Biotechnol, 151, 402–412, (2008)
  15. Kotra L.P. and Mobashery S., -Lactam antibiotics, lactamases and bacterial resistance, Bulletin de l'Institut Pasteur.,96, 139–150 (1998)
  16. Joachim M., Gerwald K., Wilma Z., Gabriele B.O., Ulrich D. and Jorg H., Evolution of microbial pathogens, Philosophical Transaction of the Royal Society.,355,695-704 (2000)
  17. Per Sandven., Epidemiology of candidemia, Rev Iberoam Micol.,17, 73-81 (2000)
  18. Hatvani N., Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture, Int. J. Antimicrob. Agents.,17, 71–74 (2001)
  19. Feresin G.E., Tapia A., Angel G.R., Delporte C., Erazo N.B. and G. Schmecla-l-lirschmann., Free radical scavengers, antiinflammatory and analgesic activity of Acaena magellanica, JPharmPharmacol., 54, 835-844(2002)
  20. Skergent M., Kotnik P., Hadolin M., Haras A.R., Simonic M. and Knez Z., Phenols, proanthocyanidins, flavones and flavonols in some plants and their antioxidant activities, Food Chem., 89, 191-198 (2005)
  21. Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen L.B., Nichols D.E. and McLaughlin J.L., Brine Shrimp: A convenient general bioassay for active plant constituents, Planta Med.,45, 31-34 (1982)
  22. Elumba Z.S., Teves F.G., Madamba M. and Reina S.B., DNA-Binding and Cytotoxic activities of Supercritical-CO2 extracts of Ganoderma lucidum (Curt.:Fr.) P. Karst. collected from the Wild of Bukidnon Province, Philippines, Int Res J Biological Sci.,2, 62-68 (2013)
  23. Krishnaraju A.V., Rao T.V.N., Sundararaju D., Vanisree M., Tsay H.S. and Subbaraju G.V., Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay, Int J Appl Sci Eng.,3, 125-134 (2005)
  24. John ES Neil JR, and Richard S. Medicinal mushrooms: a rapidly developing area of biotechnology for cancer therapy and other bioactivities, Biotechnology Letters.,24, 1839–1845 (2002)
  25. V. Venkatakrishnan., R. Shenbhagaramanb., V. Kaviyarasanb., D. Gunasundari., K. Radhika., R. Dandapani. and Loganathan K.J., Antioxidant and antiproliferative effect of pleurotus ostreatus, Journal of Phytology., 2, 022–028 (2010)
  26. Komali AS, Zheng Z, and Shetty K. A mathematical model for the growth kinetics and synthesis of phenolics in oregano (Origanum vulgare) shoot cultures inoculated with Pseudomonas species, Process. Biochem.,35, 227–235 (1999)
  27. Moller JKS, Madsen HL, Altonen T, and Skibsted LH. Dittany (Origanum dictamnus) as a source of water-extractable antioxidants, Food. Chem.,64, 215–219(1999)
  28. Gezer K, Duru ME, Kivrak I, Turkoglu A, Mercan N, Turkoglu H, and Gulcan S. Free radical Scavenging capacity and antimicrobial activity of wild –edible mushroom from Turkey, Afr. J. Biotechnol.,5, 1924-1928 (2006)
  29. Arora A, Byrem TM, Nair MG, and Strasburg GM. Modulation of liposomal membranes fluidity by flavonoids and isoflavonoids, Archives of Biochemistry and Biophysic.,373, 102-109 (2000)
  30. Blokhina O, Virolainen E, and Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress : A review, Ann Bot.,91, 179-194 (2003)
  31. Michalak Phenolic Compounds and Their AntioxidantActivity in Plants Growing under Heavy Metal Stress, Polish J of Environ Stud.,15, 523-530(2006)
  32. Sukantha T. A.,Shubashini K.S., Ravindran. and N.T., Balashanmugam. Evaluation of in vitro antioxidant and antibacterial activity of Pithecellobium dulce benth fruit peel, International Journal of Current Research.,3, 378-382 (2011)
  33. Munehiko T., Chiu W.K., Yuji N. and Takeshi T., Application of antioxidative maillrad reaction products from histidine and glucose to sardine products, Nippon Suisan Gakkaishil.,54, 1409-1414 (1998)
  34. Rahman M., Faridur, Karim M.R., Islam M.F., Habib M,R. and Hossain M.T. Phytochemical and cytotoxic investigation on oyster mushroom (P. ostreatus), International Research Journal of Pharmacy.,1, 342-345(2010)