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Genetic Relationship between the Muscids Using RAPD-PCR as marker

Author Affiliations

  • 1Cytogenetics Laboratory, Department of Zoology, University of Allahabad, UP, INDIA
  • 2Department of Zoology, C.M.P. Degree College, Allahabad, UP, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (1), Pages 66-70, January,10 (2015)


Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) markers have become the most common yardsticks to study genetic relatedness and to resolve intra and inter-specific relationship in many insect groups. In the present study, RAPD-PCR has been used to infer genetic relationship between the two muscid species i.e., housefly Musca domestica (L.) and pepper fruit fly Atherigona orientalis. Genomic DNA from the two species were extracted and PCR amplified using twenty arbitrary decamer primers which produced 127 reproducible and scorable bands ranging from 121bp to 2568bp. Genetic identity between the two species was calculated using Tools for population genetic analysis(TFPGA) software. High values of Genetic identity (0.680) display close relationship between the two species.


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