International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Preliminary Phytochemical Screening and in vitro Antimicrobial Activity of Datura stramonium Leaves Extracts Collected from Eastern Ethiopia

Author Affiliations

  • 1Department of Chemistry, Adama Science and Technology University, P.O. Box: 1888, Adama, ETHIOPIA

Int. Res. J. Biological Sci., Volume 4, Issue (1), Pages 55-59, January,10 (2015)


The present study was conducted to identify the preliminary phytochemical identification and antimicrobial investigation of leaves extracts of Datura Stramonium Linn collected from eastern Ethiopia.Datura Stramonium is traditionally used to cure different human diseases including in skin disorder, ear pain, cough, fever, burns, and asthma in Ethiopia. Phytochemical screening test in five different solvents chloroform, hexane, petroleum ether, ethanol, and acetone crude extracts were indicated the presence of flavonoids, cholesterols, terpenoids, carbohydrates, glycosides, tannins, alkaloids, phenols, proteins, and saponins. However, glycosides, phenols, and cholesterols were not detected in hexane, petroleum ether, and ethanol crude extract, respectively. Antimicrobial activity of crude extract were tested using paper disk diffusion method against four bacteria strains (S. aureus, B. subtilis, E. coli and S. typhi) and three fungi strains (F. solani, F. oxysporum and A. niger). Datura stramonium petroleum ether extract produced maximum zone of inhibition (19.300.18mm) against S. aureus while minimum zone of inhibition (12.300.16mm) against S. typhi. Hexane crude extract leaves of Datura stramonium showed maximum zone of inhibition (18.000.27mm) against S. aureus while minimum zone of inhibition (11.050.62mm) against E. coli. Chloroform extract of the plant also showed maximum zone of inhibition (18.430.57mm) against B. subtillis while minimum zone of inhibition (11.510.54mm) against S. typhi. The various crude extracts of Datura stramonium were showed high potential antifungal activity against the tested fungi with maximum zone of inhibition (17.070.16mm) against A. niger was exhibited by petroleum ether extract while minimum zone of inhibition (8.050.43mm) against F. oxysporum was observed by ethanol crude extract. The antimicrobial activities of plant extract were compared with that of chloroamphenicol against bacteria and bavistin against fungi as reference antibiotics.


  1. Stace C., New Flora of the British Isles, Cambridge University Press., 532 (1997)
  2. Ermias D., Natural Database for Africa (NDA) On CD-ROM Version 2.0, Addis Ababa University, Ethiopion, (2011)
  3. Soni P., Siddiqui A.A., Dwivedi J. and Soni V., Pharmacological properties of Datura stramonium L. as a potential medicinal tree, Asian. Pac. J. Trop. Biomed., 2(12), 1002-1008 (2012)
  4. Reddy B.U., Antimicrobial activity of Datura stramonium L. and Tylophora indica (Burm. F.) Merr, Pharmac. Online., (1), 1293-1300 (2009)
  5. Nain J., Bhatt S., Dhyani S. and Joshi N., Phytochemical screening of secondary metabolites of Datura stramonium, Int. J. Curr. Pharm. Res., 5(2), 151-153 (2013)
  6. Ivancheva S., Nikolova M. and Tsvetkova R., Pharmacological activities and biologically active compounds of Bulgarian medicinal plants, Phytochemistry, Adva. Rese., 87-103 (2006)
  7. Van Wyk B.E. and Wink M., Medicinal Plants of the World, Briza Publications, Pretoria., 123 (2004)
  8. Khan S.W. and Khatoon S., Ethnobotanical studies on some useful herbs of Haramosh and Bugrote valleys in Gilgit, Northern areas of Pakistan, Pak. J. Bot., 40(1),43-58 (2008)
  9. Njoroge G.N., Traditional Medicinal Plants in Two Urban Areas in Kenya (Thika and Nairobi): Diversity of traded species and conservation concerns, Ethno. Res. App., (9), 329-338 (2012)
  10. Dwivedi S., Dwivedi A. and Dwivedi S.N., Folk Lore Uses of Some Plants by the Tribes of Madhya Pradesh with Special Reference to Their Conservation, Ethno. Leaflet., (12), 763-71 (2008)
  11. Rahmatullah M., Islam R., Kabir Z., Rashid H., Jahan R. and Begum R., Folk Medicinal Practices in Vasu Bihar Village, Bogra District, Bangladesh, American-Eurasian J. Sust. Agric., 4(1), 86-93 (2010)
  12. Wazir S.M., Dasti A.A. and Shah J., Common medicinal plants of Chapursan valley, Gojal II, Gilgit, Pakistan, J. Res. Sci., 15(1), 41-43 (2004)
  13. Prashant T., Bimlesh K., Mandeep K., Gurpreet K. and Harleen K., A Review on phytochemical screening and extraction, Int. J. Pharm. Sci., (1), 1 (2011)
  14. Santhi R., Lakshmi G., Priyadharshini A.M. and Anandaraj L., Phytochemical screening of Nerium oleander leaves and Momordica charantia leaves, Int. Res. J. Pharm., 2(1), 131-135 (2011)
  15. Dyana J.P. and Kanchana G., Preliminary phytochemical screening of Cocos nucifera Flowers, Int. J.Curr.Pharma. Res., (4), 35 (2012)
  16. Poongothai A., Sreena K., Sreejith K., Uthiralingam M. and Annapooran S., Preliminary phytochemicals screening of Ficus racemosa, Int. J. Pharm. Bio Sci., (2), 2 (2011)
  17. Iqbal H, Moneeb U.R., Rehman K., Riaz U., Zia M., Naeem K., Farhat A., Zahoor U. and Sajjad H., Phytochemicals screening and antimicrobial activities of selected medicinal plants of Khyberpakhtunkhwa Pakistan, Africa. J. Pharm. Pharmacol., 5(6), 746-750 (2011)
  18. Rajamurugan R., Thirunavukkarasu C., Sakthivel V., Sivashanmugam M. and Raghavan C.M., Phytochemical Screening, Antioxidant and Antimicrobial Activities of Ethanolic Extract of Tecoma stans Flowers, Int. J. Pharma. Bio Sci., 4(2), 124 130 (2013)
  19. Roomiani L., Soltani M., Akhondzadeh, Basti A., Mahmoodi A., Taheri M.A. and Yadollahi F., Evaluation of the chemical composition and in vitro antimicrobial activity of Rosmarinus officinalis, Zataria multiflora, Anethum graveolens and Eucalyptusglobules against Streptococcus iniae; the cause of zoonotic disease in farmed fish, Iranian J. Fish. Sci., 12(3), 702-716 (2013)
  20. Hodges N., Pharmaceutical applications of microbiological techniques. In: M.E. Aulton, Editor, Pharmaceutics: The Science of Dosage Form Design (2nd ed.), Harcourt Publishers Limited, London, 606 (2002)
  21. Hadia G., Rubina N.Q., Muhammad A.K., Shazia H. and Nabila Y., Antibacterial and antifungal activity of different extracts of Datura stramonium (branches and leaves sample), J. Biotec. Pharm. Res., 3(9), 141-148 (2012)
  22. Alireza I., Mostafa E. and Mansour B., The Inhibitory Effects of Plant Methanolic Extract of Datura stramonium L. and Leaf Explant Callus Against Bacteria and Fungi ,Global Veter., 4(2), 149-155 (2010)
  23. Shagal M.H., Modibbo U.U. and Liman A.B., Pharmacological justification for the ethnomedical use of Datura Stramonium stem-bark extract in treatment of diseases caused by some pathogenic bacteria, Int. Res. Pharm. Pharmacol., 2(1), 016-019 (2012)