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Biosafety assessment of Pleurotus ostreatus and P. pulmonarius cultivated on Bonnylight crude oil contaminated soils

Author Affiliations

  • 1Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, NIGERIA

Int. Res. J. Biological Sci., Volume 3, Issue (6), Pages 18-23, June,10 (2014)

Abstract

This study investigated the biosafety assessment of Pleurotus ostreatus and P. pulmonarius harvested from soil contaminated with Bonnylight crude oil. Twenty seven albino rats of both sexes were allocated into three groups with three animals each viz: animals fed without P. ostreatus and P. pulmonarius (CON), animals fed with P. ostreatus harvested from soil contaminated with crude oil (POCO) and animals fed with P. pulmonarius harvested from soil contaminated with crude oil (PPCO). The animals were sacrificed after 21 days and their haematological and histopathological parameters were determined. Weights of animals fed with P. ostreatus and P. pulmonarius were higher than the control. The PCV, haemoglobin content, RBC of animals fed with POCO were higher than that of PPCO and control while WBC of animals fed with PPCO was higher than POCO and control. The heart of the rats fed with POCO extract showed little signs of histological deformation. However, there were major signs of histological abnormalities in the heart, kidney, liver and intestine of animals fed with PPCO. This study has shown that large consumption of P. ostreatus grown on crude oil contaminated soil should be done with caution to avoid slight haemorrhage and necrosis of the heart while P. pulmonarius grown on such soils should not be consumed.

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