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Effect of Ezetimibe on some biochemical factors and expression of Intestinal Scavenger receptor class B type I (SR-BI) in obsess mouse

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Author Affiliations

  • 1Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, IRAN
  • 2Physiology Research Centre, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, IRAN
  • 3Department of Biology, Islamic Azad University, Borojerd Branch, Borojerd, IRAN
  • 4Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IRAN
  • 5Department of Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, IRAN

Int. Res. J. Biological Sci., Volume 3, Issue (3), Pages 10-13, March,10 (2014)

Abstract

Ezetimibe is a new and very effective drug which reduced cholesterol. Ezetimibe well tolerated by the patients that selectively blocks cholesterol absorption from intestine.In intestine scavenger receptor class B, type I (SR-BI) has recognized as a cholesterol and triglyceride transporter.In this experiment weexamined the effect of ezetimibe on lipid profile, glucose levels as well as SR-BI expression in intestine of hypercholesterolemic mice. Mice randomly divided into three groups (n=8); group 1: hypercholesterolemic, group 2: ezetimibe and group 3: chow only. After one-month mice were sacrificed, biochemical factors were determined enzymatically as well as the levels of SR-BImRNA and protein were determined by RT-PCR and westernblotrespectively. Compared with hypercholesterolemic control, ezetimibe significantly decreased low-density lipoprotein cholesterol (LDL-C) (P0.05) and total cholesterol (P0. 05). Intestinal SR-BI mRNA and protein were significantly decreased in intestine by ezetimibe (P0.05). Taken together, ezetimibe significantly reduced total cholesterol as well as led to down-regulation of SR-BI in mouse intestine.

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