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Effects of Curcuma longa and Cinnamon aqueous extracts on Serum Carbohydrates and Lipids metabolism and oxidative status in high Fructose fed Rats

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Author Affiliations

  • 1Department of Biochemistry, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 2 Student Research Committee, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 3Department of Anatomy, Medical School, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 4Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IRAN

Int. Res. J. Biological Sci., Volume 3, Issue (3), Pages 78-82, March,10 (2014)

Abstract

This study was designed to assess the effect of Curcuma longa (turmeric) and cinnamon extract on the blood glucose, insulin, lipid profile levels and oxidative status in high fructose-fed rats. Forty rats of 5-8 weeks old were divided into five groups with 8 rats in each group. Each group was fed with different diets as follows Group 1: common diet (Cont); Group 2: 21% fructose (Fru); Group 3: 10% turmeric (0.3ml/day/rat) with 21% Fru (Fru + Tur-1); Group 4: 10% turmeric (3ml/day/rat) with 21% Fru (Fru+ Tur-2); and Group 5:healthy rats consuming 10% turmeric (3ml/day/rat) (Cont + Tur-2). Cinnamon also followed the same grouping (Cont, Fru, Fru + Cin-1, Fru + Cin-2 and Cont + Cin-2). The experimental feeding was continued for 10 weeks. Treatment with cinnamon and curcuma (turmeric) was administered orally from the 21th day of fructose feeding. The animals were sacriced. Then abdominal incision was given and blood was collected from the jugular vein and serum was separated and stored at 80°C. The serum glucose, ALT, AST, Cholesterol, Triglyceride, and HDL-C were determined using Pars Azmun kit (Iran), and LDL-C was calculated. Insulin was assayed using an immunoassay kit. NO was measured by ENZO NO Parameter Assay Kit. Total antioxidant capacity (TAC) was determined using ferric reducing antioxidant power assay (Frap). Maolnedialdehyde (MDA) as a marker for lipid peroxidation was measured using a flurometric method. Total oxidant status (TOS) in serum samples was determined using the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement of the ferric ion by xylenol orange. Feeding rats with high fructose diet leads to increase in glucose and insulin. There was an increase in cholesterol, triglyceride and LDL-C in fructose fed rats as compared to controls. Treatment with turmeric and cinnamon extract significantly reduced these parameters. These ndings indicate the improvement of blood glucose, insulin resistance, lipid profiles and antioxidant activity by Curcuma longa (Turmeric) and Cinnamon in high fructose-fed rats.

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