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Analysis of variability in Hemolymph protein pattern in four populations of wild and semi-domestic ecoraces of Antheraea mylitta Drury (Lepidoptera: Saturniidae)

Author Affiliations

  • 1Silkworm Breeding and Genetics, Central Tasar Research and Training Institute, Central Silk Board, Ranchi, Jharkhand, 835303, INDIA
  • 2 Department of Biotechnology, Marwari College, Ranchi University, Ranchi, Jharkhand- 834002, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (11), Pages 42-46, November,10 (2014)

Abstract

Tropical tasar silkwormAntheraea mylitta Drury is an important wild sericigenous insect species encountered diverse tropical ecological conditions in different eco-pockets and evolved as ecoraces with wide variations in phenotypic and behavioural characters. Four populations from two important ecoraces were analysed for variability in the pupal hemolymph electrophoretic protein pattern. The hemolymph from Daba wild and semi-domestic population as well as wild and reared populations of Laria ecorace was drawn and used for the study. Protein concentration was highly variable and significant (at p0.05) between the different groups studied. Higher protein concentration was recorded in the hemolymph of Daba (wild) female pupae (235.3 ± 1.72 mg/ml) followed by Daba (Semi-domestic) female hemolymph (225.4 ± 3.04 mg/ml) was recorded. Variations observed in the number of protein bands in SDS-PAGE analysis of male and female pupal hemolymph of different populations. Appearance of 35 kDa Protein band was significant in the female hemolymph samples of all the populations. The protein bands 98 kDa (storage proteins in lepidopterons), 45 kDa, 43 kDa, 20 kDa and 14 kDa were found to be common in all the hemolymph samples. More number of protein bands was observed in the hemolymph samples of Daba between 20 kDa to 98 kDa compared to the samples of Laria populations. The greater variability in number of bands observed between 96 kDa to 99 kDa. This indicates high heterogeneity with respect to the food plant, region and also in sexual dimorphism.

References

  1. Suryanarayana N and Srivastava A.K, Monograph on Tropical Tasar Silkworm. Central Tasar Research and Training Institute, CSB, India (2005)
  2. Gamo T., Recent concepts and trends in silkworm breeding, Farming, Japan, 10(6), 11-12 (1976)
  3. Lokesh G., Narayanaswamy M and Ananthanarayana S.R., The Effect of Chemical Mutagen on Hemolymph Proteins of silkworm, Bombyx mori L (Lepidoptera : Bombycidae) in F1 Stage, J. Appl. Sci. Environ. Mgt., 10(3), 21-25 (2006)
  4. Lokesh G and Ananthanarayana S.R., Changes in the Protein profile of silkworm Bombyx mori. L (Lepidoptera: Bombycidae) in response to the chemical mutagen, Int. J. Sci. Nat,2(3), 559-563 (2011)
  5. Shivakumar B and Subramanya G, Electrophoretic hemolymph protein pattern in a few bivoltine races of the Silkworm, Bombyx mori. The Bioscan, 5(4), 541 – 544 (2010)
  6. Lokesh. G, Sushma R Tirkey, Srivastava A.K, Kar P.K and Sinha M.K., Biochemical Performance and Quantitative Assessment of F1 Hybrid Of Two Ecoraces of Tropical Tasar Silkworm Antheraea mylitta Drury (Lepidoptera : Saturniidae), Int. J. Indust. Entomol.,26(2), 67-73 (2013)
  7. Kumar D, Pandey J.P, Jain J, Mishra P.K. and Prasad B.C, Qualitative and Quantitative changes in protein profile of various tissue of tropical tasar silkworm, Antheraea mylitta Drury. Int. J. Zool. Res,7(2), 147 -155 (2011)
  8. Takasusuki Viana L.H.D., Baitala T.V, Nicolin K.C. and Toledo V.A.A., Characterization of esterases in Tetragonisca angustula and Tetragona clavipes, Broc. J. morphol. Sci.,23(3 and 4), 431-434 (2006)
  9. Frey K.J., Cox T.S., Rodgers D.M. and Cox P.M, Increasing cereal yields with gene from wild and weedy species, Proc. XV Intl. Cong. Genetics, 4, 51-68 (1983)
  10. Zeng Zhongren wang, Jif Shhijang Zhou, Jiayu Bai and Haishui Zheng, Allozyme variation and population genetic structure of Betula alnoides from Guangxi, China, Biochemical Genetics, 41, 61-75 (2003)
  11. Doddaswamy M.S. and Subramanya G., Hemolymph protein profiles in three pure races and their hybrids of the silkworm, Bombyx mori L. through SDS-PAGE, The bioscan, 2(3), 189-193 (2007)
  12. Somasundaram P, Ashok Kumar K, Thangavelu K, Kar P.K. and Sinha R.K, Preliminary study of Isozyme variation in silkworm Germplasm of Bombyx mori (L) and its implication for conservation, Pertanika J. Trop. Agric. Sci., 27 (2), 163-171 (2004)
  13. Sreerama Reddy G and Subramanya G, Genetic differences between the mutant with shorter larval duration and the standard in Pure Mysore race of Silkworm Bombyx mori L, Sericologia. 196 -202 (1982)
  14. Hegde S.N. and Krishnamurthy N.B., Ontogenetic differentiation of alkaline and acid phosphatases in two races of silkworm Bombyx mori (L), The Indian zoologist, 4(1,2), 27-32 (1980)
  15. Sande V. and Karcher D., Species differentiation of insect by haemolymph electrophoresis, Science,131, 1103 (1960)
  16. Hubby J.L. and Lewontin R.C., A molecular approach to the study of genic heterozygocity in natural Populations. I. The number of alleles at different loci in Drosophila Pseudobscura. Genetics, 54, 577-594 (1966)
  17. Lokesh G, Kar P.K, Srivastava A.K, Saloni Swaroopa and Sinha M.K., Studies on the high temperature induced stress on the biochemical profile and fecundity of Daba and Laria ecoraces of tropical ilkworm Antheraea mylitta Drury (Lepidoptera: Saturniidae), Int. J. Indust. Entomol, 24(1), 69-74 (2012 b)
  18. Lowry O.H, Rosebrough N.J, Farr A.L and Randall R.J., Protein measurement with Folin phenol reagent, J. Biol. Chem., 193, 267-275 (1951)
  19. Zingales B, Analysis of proteins by Sodium Dodecyl Sulphate-PolyAcrylamide Gel Electrophoresis In : Genes and antigens of parasites (Ed. By Morel C. M.). 2nd Edn. Fundacao Oswaldo Cruz, Rio’de Janeiro, Brazil, 357-363 (1984)
  20. Ayala F.J., Powell J.R., Tracey M.L., Mourao C.A. and Sala S.P., Enzyme variability in Drosophila willistoni group VI Genic variation in natural populations of Drosophila willistoni, Genetics, 70, 113-139 (1974)
  21. Lewontin R.C. and Hubby J.L., A molecular approach to the study of genic heterozygocity in natural population ll. Amount of variation and degree of heterozygocity in natural populatios of Drosophila Pseudovscura. Genetics, 54, 595-609 (1966)
  22. Hirobe T., Evolution, differentiation and breeding of the silkworm- the silk road, past and present –Genetics in Asian countries, XII Int. Cong. Genet., 25-36 (1968)
  23. Hurliman R.F. and Chen P.S, Ontogenetische Veranderungen des enzymmusters in der hemolymphe von Phormia rigina. Revue. Swisse. Zool., 81, 648-654 (1974)
  24. Babu K.R., Ramakrishna S., Reddy Y.H.K, Lakshmi G., Naidu N.V., Basha S.S. and Bhaskar M., Metabolic alterations and molecular mechanism in silkworm larvae during viral infection : A review. Afr. J. Biotechnol., , 899-907 (2009)
  25. Kumar N.K., Ismail S.M and Dutta A., Differential uptake of storage protein by the fat body of Rice moth, Corcyra cephalonicaduring the larval pupal development, Entomon, 23, 83-90 (1998)
  26. Lokesh G, Putkho Paul Pao, Madhusudhan K.N, Kar P.K, Srivastava A.K, Sinha M.K, Manohar Reddy R, Muniswamy Reddy P.M and Prasad B.C., Study of Phenotypic Variability in Silk Gland Characters in Three Ecoraces of Tropical Silkworm Antheraea mylitta Drury, Asian Journal of Animal and Veterinary Advances, 7(1), 80-84 (2012a)
  27. Srivastava A.K, Kar P.K, Naqvi A.H, Sinha A.K, Singh B.M.K, Sinha B.R.R.P and Thangavelu K, Biochemical variation in ecoraces of Antheraea mylitta : A review, Perspectives in Cytology and Genetics, 10, 359-346 (2001)
  28. Venugopal Pillai S, Krishnaswami S and Kashivishwanathan K., Growth studies in silkworm, Bombyx mori. L. under tropical conditions, II. Influence of agronomical methods of mulberry on growth, cocoon crop and fecundity of silkworm, Ind. J. Seric. 26(1), 38-45 (1987)
  29. Srivastava A.K, Naqvi A.H, Roy G.C. and Sinha B.R.R.P., Temporal variation in quantitative and qualitative characters of Antheraea mylitta Drury, International journal of wild silkmoth and silk, , 54 -56 (2000)
  30. Srivastava A.K, Naqvi A.H, Sinha AK, Vishwakarma S.R. and Roy G.C., Genotype and environment interaction in Antheraea mylitta Drury and its implications, Perspectives in Cytology and Genetics, 11, 219- 224 (2004)
  31. Fujiwara Y. and Yamashita O., Purification, characterization and developmental changes in the titer of a new larval serum protein of the silkworm, Bombyx mori. Insect. Biochem., 20, 751-759 (1990)
  32. Sakurai S., Temporal organization of endocrine events underlying larval pupal metamorphosis in the silkworm, Bombyx mori L., J. Insect. Physiol., 30, 657-664 (1984)