International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Bioactivity of Endophytic Fungus Colletotrichum gloeosporioides Isolated from Phlogacanthus thyrsiflorus Nees

    ,

Author Affiliations

  • 1Department of Life Sciences, Manipur University, Manipur-795003, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (10), Pages 97-99, October,10 (2014)

Abstract

To study antibacterial, antioxidant and total phenolic content of Colletotrichum gloeosporioides an endophytic fungus isolated from Phlogacanthus thyrsiflorus Nees. Fungal endophytes were isolated from Phlogacanthus thyrsiflorus Nees collected from Imphal West, Manipur. Morphological and molecular identification was done for the potential isolate. Extract from fermentation broth of Colletotrichum gloeosporioides were checked for antibacterial activity, antioxidant activity and total phenolic content. Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC25922) and Pseudomonas aeruginosa (ATCC27853) were used for testing antibacterial activity. The crude extract showed an effective antibacterial activity against S. aureus with a zone of inhibition of 13.33±1.52mm. The crude extract of an endophytic fungus Colletotrichum gloeosporioides was studied for total phenolic content and antioxidant activity. Free radical scavenging activityof the extract showed 90%. Total phenolic content is 18.3±0.36mg gallic acid/g of extract. This report of antibacterial and antioxidant activity of endophytic fungus Colletotrichum gloeosporioides residing in Phlogacanthus thyrsiflorus Nees is presented for the first time in this work. The findings indicate that the metabolite of endophytic fungus Colletotrichum gloeosporioides is a potent source of bioactive compounds. Further study is needed for identification of the bioactive compounds.

References

  1. Vaz A.B. Mota R.C. Bomfim M.R. Vieira ML, Zani CL, Rosa C.A, Rosa L.H., Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil, Can J Microbiol, 55, 1381-1391 (2009)
  2. Petrini O., Fungal endophytes of tree leave. In: Andrews JA, Hirano SS (eds), Microbial ecology of leaves. Springer, New York., 179–197 (1991)
  3. Yan X.N. Sikora I.R. Zheng J.W., Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita, J Zhejiang Univ-Sci B, Biomed & Biotechnol, 12(3), 219–225 (2011)
  4. Tan R.X. Zou W.X., Endophytes: a rich source of functional metabolites, Natural Product Reproduction, 18, 448–459 (2001)
  5. Kumar D.S.S. Lau C.S. Wan J.M.F. Yang D. Hyde K.D., Immunomodulatory compounds from Pestalotiopsis leucoth? (HKUCC 10197), an endophytic fungus of Tripterygium wilfordii, Life Sciences., 78, 147–156 (2005)
  6. Strobel G.A. Hess W.M. Ford E. Sidhu R.S.Yang X., Taxol from fungal endophyte and issue of biodiversity, Journal of Industrial Microbiology, 17, 417-423 (1996)
  7. Devi N.N. Prabakaran J.J. and Femina W., Phytochemical Analysis and enzyme analysis of endophytic fungi from Centella asiatica, Asian Pacific Journal of Tropical Biomedicine, S1280-S1284 (2012)
  8. Warjeet S.L, Brajeshwari D.K., Studies on the chemical constituents and antioxidant activities of the extracts from the roots of Smilax lancaefolia Roxb, Natural Product Res., 24(12), 1168-1176 (2010)
  9. Strobel G.A., Endophytes as sources of biocative products, Microbes infection, 5, 535-544 (2003)
  10. Schulz B. Boyle C. Draeger S. Rommert A.K. Krohn K., Endophytic fungi: A source of novel biologically active secondary metabolites, Mycol Res ., 106, 996–1004, (2002)
  11. Photita W. Taylor P.W.J. Ford R. Hyde K.D. and Lumyong S., Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand, Fungal Diversity, 18, 117-133 (2005)
  12. Devarajan P.T. and Suryanarayanan T.S., Evidence for the role of phytophagous insects in dispersal of non – grass fungal endophytes, Fungal Diversity, 23, 111-119 (2006)
  13. Kumar S. Kaushik N. Edrada-Ebel R. Ebel R. and Proksch P., Isolation, characterization and bioactivity of endophytic fungi of Tylophora indica , World Journal of Microbiology and Biotechnology., 27( 3), 571-577 (2011)
  14. Zou W.X. Meng J.C. Lu H. Chen G.X. Shi G.X. Zhang T.Y.Tan R.X., Metabolites of Colletotrichum gloeosporioides, an Endophytic Fungus in Artemisia mongolica, Journal of Natural Products., 63, 11 (2000)
  15. Photita W.Lumyong S. Lumyong P. Hyde K.D., Endophytic fungi of wild banana (Musa acuminata) At Doi Suthep Pui National Park, Thailand, Mycol. Res., 105, 1508-1513 (2001)
  16. Lu H.Zou W.X.Meng J.C.Hu J.Tan R.X.,New bioactive metabolites produced by Colletotrichum sp. an endophytic fungus in Artemisia annua, Plant Sci., 151, 7673 (2000)
  17. Wang Y.T., Lo H.S., Wang P.H., Endophytic fungi from Taxus mairei in Taiwan: first report of Colletotrichum gloeosporioides as an endophyte of Taxus mairei, Botanical Studies, 49, 39-43 (2008)
  18. Lima J.S. Figueiredo J.G. Gomes R.G. Stringari D. Goulin E.H. Adamoski D. Kava-Cordeiro V. Galli-Terasawa L.V. Glienke C. Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree. ISRN Microbiology, 2012, Article ID 215716, 7 (2012)
  19. Strobel G., Daisy B., Castillo U. and Harper J., Natural products from endophytic microorganisms, Journal of Natural Products., 67, 257-268 (2004)
  20. Zhang H.W. Song Y.C. Tan R.X., Biology and chemistry of endophytes, Natural Product Reports., 23, 753-771(2006)