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To evaluate Lab scale Cultivation of Spirulina by using different substrates and to Evaluate its Chlorophyll and Protein content

Author Affiliations

  • 1 Biotechnology Department, University Institute of Sciences, Chandigarh University, Gharuan, Mohali, Punjab, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (1), Pages 22-30, January,10 (2014)

Abstract

Spirulina is a multicellular, filamentous, free floating cyanobacterium or photosynthetic blue green algae. Spirulina has been so popular in the world due to its high nutritional contents. As it contains chlorophyll ‘a’, like higher plants botanist classify it as micro algae belonging to Cyanophyceae class; but according to bacteriologists it is a bacterium due to its prokaryotic structure. Mexicans started using this microorganism as human food. The nutritional status of cultured Spirulina suggested that the biomass of Spirulina is nutritionally rich in protein. Cyanobacterium Spirulina is proficient to cultivate in a variety of culture media. The present investigation is carried out to evaluate the lab scale cultivation of Spirulina by using different substrates like cheese whey, cow urine, rain water and tube well water. Also it is evaluated for its chlorophyll and protein content. Different concentration gradients of the substrates i.e. cheese whey, cow urine, rain water and tube well water ranges from 10-1 to 10-6 with ZARROUK’S medium were analyzed for Spirulina growth at pH 9.5 ± 2, temperature 30°C ± 2 and photo-period of 10-12 hours. Spirulina was successfully cultivated by using different substrates and maximum chlorophyll and protein content was founded. The results indicate the potentiality of all the four substrates to provide nutrients to culture medium that reduces its valuable cost and make it a cheaper and economic medium to cultivate Spirulina.

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