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Diethanolamine Cytotoxicity on Red Blood Corpuscles

Author Affiliations

  • 1 Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad-380009, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (1), Pages 67-69, January,10 (2014)

Abstract

The purpose of this study was to evaluate the effect of diethanolamine (DEA) on red blood corpuscles (RBC) in in vitro condition. Intravenous blood samples from healthy adult volunteers (age 25-30 years) were collected in EDTA vials and used for preparation of RBC suspension in saline (0.9% NaCl). RBC suspension was incubated with different concentrations (100-1000 µg/ml) of diethanolamine at 37 ºC for 4 h. Morphological alterations and percent hemolysis were recorded. Statistical analysis was performed using the analysis of variance (ANOVA) followed by Tukey’s test and the level of significance was accepted with * p0.05. The results showed that diethanolamine caused concentration-dependent significant increase (P0.05) in hemolysis.

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