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Assessment of Parameters Influencing Rice Straw Associated Mycelial Growth of Pleurotus ostreatus MTCC 142 and a Wild Isolate of Pleurotus ostreatus

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Author Affiliations

  • 1Dept. of Microbiology, Karpagam University, Coimbatore-641021, Tamil Nadu, INDIA
  • 2 Dept. of Microbiology, Genohelix Biolabs, CASB, Jain University, Bangalore-560019, Karnataka, INDIA
  • 3 Dept. of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA
  • 4

Int. Res. J. Biological Sci., Volume 2, Issue (9), Pages 15-21, September,10 (2013)

Abstract

Pleurotus ostreatus, commonly known as the oyster mushroom, is a medicinally and biotechnologically important edible mushroom. The present study focuses on assessment of various parameters which may influence the optimum mycelial growth of Pleurotus ostreatus MTCC 142 and a wild isolate of Pleurotus ostreatus. Solid-state cultivation of the mushroom was carried out using rice straw as the substrate. Indirect methods of measurement such as cellulolytic activity and total protein content were used to determine the level of rice straw associatedmycelial growth. In case of Pleurotus ostreatus MTCC 142 the highest protein yield was recorded in rice straw supplemented with 1% (w/v) potato starch and 0.5% (w/v) peptone, with an initial moisture ratio of 1:10, whereas, the highest cellulase production was associated with rice straw supplemented with 1% (w/v) lactose and 0.5% (w/v) tryptone, with an initial moisture ratio of 1:5, when inoculated with one mycelium plug. For the wild isolate of Pleurotus ostreatus the highest protein content and cellulase activity were recorded in the substrate supplemented with 1% (w/v) lactose and 0.5% (w/v) beef extract, with an initial moisture ratio of 1:10, when inoculated with two mycelial plugs.

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