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In vitro Pollen Viability and Pollen Germination in Medlar (Mespilus germanica L.)

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Author Affiliations

  • 1 Kocaeli University, Arslanbey Agricultural Vocational School, TR-41285, Kocaeli, TURKEY

Int. Res. J. Biological Sci., Volume 2, Issue (5), Pages 49-53, May,10 (2013)

Abstract

The study was carried out to determine in vitro pollen viability and pollen germination of 4 cultivar and 4 wild types of medlar (Mespilus germanica L.) plants. Two pollen viability tests (TTC and IKI) and one in vitro pollen germination test (Agar Plate) at four different germination media were used in the study. The viability varied significantly according to the genotypes of medlar and tests used. However pollen viability in the IKI test was generally higher (84.83-100 %) and stable than TTC test (11.78-91.66 %) in freshly obtained pollen. In the second step, pollen viabilities of six months stored at +4 C and freshly obtained pollen were compared at IKI test. After 6 months storage at +4 C, pollen viability was highly maintained (72.22 %- 94.05 % for all genotypes). Therefore no significantly difference observed among storage time or plant types. The fresh pollen germination in agar plate tests varied between (16.4 %-66.67 % ) for all germination media and plant types but any significantly difference was seen among the media or plant types.

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