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DNA-binding Activity and Partial Characterization by Fourier Transform Infrared Spectroscopy (FTIR) of Curcuma longa L. SC-CO2 Extracts

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Author Affiliations

  • 1 Dept. of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES

Int. Res. J. Biological Sci., Volume 2, Issue (5), Pages 40-44, May,10 (2013)

Abstract

Nowadays, an increasing interest in a number of plants for their medicinal properties had been shown by a large number of scientific studies conducted. Turmeric (Curcuma longa Linn.) is among those studied plants demonstrating potential medicinal properties. In this study, SC-CO2 extracts of C. longa L. using a local variety were characterized and studied for their DNA-binding activity using the Biomolecular-chemical screening method. Results showed Rf-ratio values ranges from 0.50-0.83 which indicate the presence of compounds with strong to moderate affinity towards DNA. Fourier Transform Infrared Spectroscopy was employed for the detection and characterization of the compounds present in the extracts. FTIR results of C. longa L. extracts at 10MPa, 20MPa and 30MPa revealed almost the same high peak levels which indicate the presence of the same functional groups O-H stretching in phenols, -C-H stretching of alkanes and C=O stretch of carbonyl groups. Presence of these functional groups and DNA-binding affinity exhibited by C. longa L. towards DNA suggests that the plant has a potential pharmacological property.

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