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Sequence Analysis of Putative luxS Gene Involved in Prodigiosin Biosynthesis from Philippine Local Strains of Serratia marcescens

Author Affiliations

  • 1 Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City 9200, PHILIPPINES

Int. Res. J. Biological Sci., Volume 2, Issue (4), Pages 13-19, April,10 (2013)

Abstract

Prodigiosin produced by Serratia marcescens is a bacterial metabolite that has antibiotic, immunosuppressive and anticancer properties involving the luxS gene. The putative luxS gene of the five local S. marcescens strains (B1748, B111, B112, B211, and B212) was amplified by PCR and sequenced and their nucleotide and amino acid sequences were characterized through bioinformatics. The resulting putative luxS nucleotide sequences of the five local strains are highly similar to the nucleotide sequence of two strains of S. marcescens in the EMBL database (Acc. No. EF164926.1 and Acc. No. AJ628150.1) having a maximum identity of 98%, 94%, 97%, 98%, and 100% respectively. However, the five local strains were more related to the clade of EF164926.1 than that of AJ628150.1 using neighbor-joining method of MEGA ver.5. BLASTP Homology search was done and B1748 and B212 strains showed a high degree of homology (100%) with a protein product of the luxS gene of Neisseria gonorrhoeae and Salmonella enterica correspondingly. In addition, a putative conserved domain was detected in all protein sequences from the local strains of S. marcescens. The conserved domain was in the luxS superfamily consisting of the luxS protein involved in autoinducer AI-2 synthesis and its hypothetical relatives. Results of in silico analyses used in this study confirmed presence of putative luxS gene in local strains of S. marcescens with high potential prodigiosin production needed in the manufacture of pharmacological important products. This is the first report in the Philippines on the presence of luxS gene from local isolates of S. marcescens.

References

  1. Grimont P.A. and Grimont F., The genus Serratia, Annu Rev Microbiol.,32, 21–248 (1978)
  2. Stock I., Thomas G. and Bernd W.,Natural antibiotic susceptibility of strains of Serratia marcescens and the S. liquefaciens complex: S. liquefaciens sensu stricto, S. proteamaculans and S. grimesii,Int J Antimicrob Agents,22, 35 (2003)
  3. Gerber N.N., Prodigiosin-like pigments, CRC Crit. Rev. Microbiol., 469-485 (1975)
  4. Williams R.P., Gott C.L., Qadri S.M. and Scott R.H., Influence of temperature of incubation and type of growth medium on pigmentation in Serratia marcescens, J Bacteriol.,106, 438–443 (1971)
  5. Bu'Lock, J.D.,Intermediary metabolism and antibiotic synthesis, Annu Rev Microbiol.,, 293±342 (1961)
  6. Demain A.L., Why do microorganisms produce antimicrobials? In fifty years of antimicrobials: past perspectives and future trends (Society for General Microbiology Symposium no. 53), pp. 205–228. Edited by P. A. Hunter, G. K. Darby & N. J. Russell. Cambridge: Cambridge University Press. (1995)
  7. Williams R.P. and Quadri S.M., The pigments of Serratiain the genus Serratia, pp. 31–75. Edited by A. Von Graevenitz & S. J. Rubin. Boca Raton, FL: CRC Press Inc. (1980)
  8. Manderville R.A., Synthesis, proton-affinity and anti-cancer properties of the prodigiosin-group natural products, Curr Med Chem Anticancer Agents,, 195–218 (2001)
  9. Perez-Tomas R., Montaner B., Llagostera E. and SotoCerrato V., The prodigiosins, proapoptotic drugs with anticancer properties, Biochem Pharmaco.,66, 1447 – 52 (2003)
  10. Slater H., Crow M., Everson L. and Salmond G.P., Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways, Mol Microbiol.,47, 303–320 (2003)
  11. Vendeville A., Winzer K., Heurlier K., Tang C.M. and Hardie K.R., Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria, Nature Rev. Microbiol., 383–396 (2005)
  12. Whitehead, N.A., Barnard A.M., Slater H., Simpson N.J. and Salmond G.P.,Quorum-sensing in gram-negative bacteria, FEMS Microbiol Rev., 25, 365–404 (2001)
  13. . Coulthurst S.J., Kurz C.L. and Salmond G.P., LuxS mutants of Serratia defective in autoinducer-2-dependent ‘quorum sensing’ show strain-dependent impacts on virulence and production of carbapenem and prodigiosin, Microbiology,150, 1901–1910 (2004)
  14. Han S.B., Kim H.M., Kim Y.H., Lee C.W., Jang E.S., Son K.H., Kim S.U. and KIM Y.K., T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens, Int. J. Immunopharmacol., 20, 1–13 (1998)
  15. Montaner B., Navarro S., Piqué M., Vilaseca M., Martinell M., Giralt E., Gil J. and Peréz-Tomás R., Prodigiosin from the supernatant of Serratia marcescens induces apoptosis in haematopoietic cancer cell lines, Br JPharmacol., 131, 585–593 (2000)
  16. Williams R.P., Biosynthesis of prodigiosin, a secondary metabolite of Serratia marcescens,Appl. Microbiol., 25, 396–402 (1973)
  17. Cerdeño A.M., Bibb M.J. and Challis G.L., Analysis of the prodiginine biosynthesis gene cluster of Streptomyces coelicolor A3 (2): new mechanisms for chain initiation and termination in modular multienzymes, Chem Biol.,, 817–829 (2001)
  18. Thomas M.G., Burkart M.D. and Walsh C.T., Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis., Chem Biol., 171–184 (2002)
  19. Xavier K.B. and Bassler B.L., LuxS quorum sensing more than just a numbers game, Curr. Opin. Microbiol., pp. 191–197 (2003)
  20. Pei D.H. and Zhu J.G., Mechanism of action of S-ribosylhomocysteinase (LuxS), Curr Opin Chem Biol.,, 492–497 (2004)
  21. Winzer K., Hardie K.R. and Williams P., LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria, Adv. Appl. Microbiol., 53, 291–396 (2003)
  22. Medina M., Urdiales J.L. and Amores-Sanchez M.I., Roles of homocysteine in cell metabolism: old and new functions, Eur J Biochem., 268, 3871-3882 (2001)