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Synthesis, Characterization and Evaluation of Antimicrobial Efficacy of Silver Nanoparticles using Paederia foetida L. leaf extract

Author Affiliations

  • 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam-638 401, Erode District, Tamil Nadu, INDIA
  • 2 Department of Environmental Engineering, BK21 Team for Biohydrogen Production and Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759, REPUBLIC OF KOREA

Int. Res. J. Biological Sci., Volume 2, Issue (3), Pages 28-34, March,10 (2013)

Abstract

The present study reports the synthesis of silver nanoparticles using Paederiafoetida L. leaf extract were used as reducing agent from silver nitrate solution. The synthesis of silver nanoparticles was analyzed by UV-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy coupled with Energy Dispersive X-ray Analysis (SEM-EDX). The synthesized silver nanoparticles were spherical in shape with an average size of 24nm. The antimicrobial activity of the silver nanoparticles shows the diameter of inhibition zones around the disk for Staphylococcus aureus, Klebsella sp., V. cholera, P. aerueginosa and E. coli bacterial suspension are 14mm, 26mm, 12mm, 24mm, and 16mm respectively. Among the four bacterial species, Klebsella sp. and P. aerueginosa was showed higher zone of inhibition 26mm and 24mm, respectively. The results were compared with the ciprofloxacin positive control and synergistic effect of silver nanoparticles with ciprofloxacin. In the concluding remarks, the silver nanoparticles synthesized using P. foetida leaf extract would be a better antimicrobial activity against various bacterial species, when applied as individual or combined with commercial antibiotic.

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