6th International Young Scientist Congress (IYSC-2020) and Workshop on Intellectual Property Rights. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Synthesis, Characterization and Evaluation of Antimicrobial Efficacy of Silver Nanoparticles using Paederia foetida L. leaf extract

Author Affiliations

  • 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam-638 401, Erode District, Tamil Nadu, INDIA
  • 2 Department of Environmental Engineering, BK21 Team for Biohydrogen Production and Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759, REPUBLIC OF KOREA

Int. Res. J. Biological Sci., Volume 2, Issue (3), Pages 28-34, March,10 (2013)


The present study reports the synthesis of silver nanoparticles using Paederiafoetida L. leaf extract were used as reducing agent from silver nitrate solution. The synthesis of silver nanoparticles was analyzed by UV-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy coupled with Energy Dispersive X-ray Analysis (SEM-EDX). The synthesized silver nanoparticles were spherical in shape with an average size of 24nm. The antimicrobial activity of the silver nanoparticles shows the diameter of inhibition zones around the disk for Staphylococcus aureus, Klebsella sp., V. cholera, P. aerueginosa and E. coli bacterial suspension are 14mm, 26mm, 12mm, 24mm, and 16mm respectively. Among the four bacterial species, Klebsella sp. and P. aerueginosa was showed higher zone of inhibition 26mm and 24mm, respectively. The results were compared with the ciprofloxacin positive control and synergistic effect of silver nanoparticles with ciprofloxacin. In the concluding remarks, the silver nanoparticles synthesized using P. foetida leaf extract would be a better antimicrobial activity against various bacterial species, when applied as individual or combined with commercial antibiotic.


  1. Huang J., Li Q., Sun D., Lu Y., Su Y., Yang X., Wang H., Wang Y., Shao W., He N., Hong J. and Chen C., Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf, Nanotechnology, 18,105104-105114 (2007)
  2. Salam H. A., Rajiv P., Kamaraj M., Jagadeeswaran P., Sangeetha Gunalan and Sivaraj R., Plants: Green Route for Nanoparticle Synthesis, International Research Journal of Biological Sciences, 1(5), 85-90 (2012)
  3. Dwivedi A.D., Gopal K., Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 369, 27-33 (2010)
  4. Krishnaraj C., Jagan E. G., Rajasekar S., Selvakumar P., Kalaichelvan P.T., Mohan N., Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens, Colloids and Surfaces B: Biointerfaces, 76, 50 56 (2010)
  5. Veerasamy R., Xin T. Z. Subashini Gunasagaran, Xiang T. F. W., Yang E. F.C., Jeyakumar N., Dhanaraj S. A., Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities, Journal of Saudi Chemical Society, 15, 113120 (2011)
  6. Phanjom P., Zoremi D.Z., Mazumder J., Saha M., Baruah S.B., Green Synthesis of Silver Nanoparticles using Leaf Extract of Myrica esculenta, International Journal of NanoScience and Nanotechnology. , 73-79 (2012)
  7. Li S., Shen Y., Xie A., Yu X., Qiu L., Zhang L., Zhang Q., Green synthesis of silver nanoparticles using Capsicum annuum L. extract, Green Chemistry, 852-858 (2007)
  8. Shankar S. S., Rai A., Ahmad A., Sastry M., Biosynthesis of silver and gold nanoparticles from extracts of different parts of the geranium plant, Applied Nano Science, 69-77 (2004)
  9. Song J. Y., Kim, B. S., Biological synthesis of bimetallic Au/Ag nanoparticles using Persimmon (Diopyros kaki) leaf extract, Korean Journal of Chemical Engineering, 25, 808-811 (2008)
  10. Song J. Y., Jang, Kyeong H., Kim B. S., Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts, Process Biochemistry, 44, 1133-1138 (2009)
  11. Narayanan K. B., Sakthivel N., Coriander leaf mediated biosynthesis of gold nanoparticles, Materials Letter, 62, 4588-4590 (2008)
  12. Khushbu C., Anar P., Mayuree P., Carol M., Roshni S., Subodh A., Paederia foetida Linn. As a potential medicinal plant: A Review, Journal of Pharmacy Research, 3135-3137 (2010)
  13. Samy R. P, Ignacimuthu S., Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India,Journal of Ethnopharmacology,69, 63-71 (2000)
  14. Chen Y., Wang C., Ma Z., Su Z., Controllable colours and shapes of silver nanostructures based on pH: application to surface-enhanced Raman scattering , Nanotechnology, 18, 325602 (2007)
  15. Cruz D., Fale P. L., Mourato A., Va P. D., Serralheiro M. L., Lino A. R., Preparation and physicochemical characterization of Ag nanoparticles biosynthesized by Lippia citriodora (Lemon Verbena), Colloids Surf. B: Biointerfaces, 81, 67-73 (2010)
  16. Klaus T., Joerger R. Olsson E., Granqvist C. G. Silver-based crystalline nanoparticles, microbially fabricated. Proceedings of the National Academy of Sciences of the United States of America. USA 96, 13611-13614 (1999)
  17. Nicholas L., Ansari S., Francis R. Livens., Joanna C. Renshaw, Jonathan R. Lloyd., Formation of nanoscale elemental silver particles via enzymatic reduction by Geobacter sulfurreducens, Applied and Environmental Microbiology, 74, 7090-7093 (2008)
  18. Hwan K. S, Hyeong-Seon L., Deok-Seon R., Soo-Jae C., Dong-Seok L., Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli, Korean Journal of Microbiology and Biotechnology, 39, 77-85 (2011)
  19. Shirley A., Dayanand B., Sreedhar., Dastager S. G., Antimicrobial activity of silver nanoparticles synthesized from novel Streptomyces species, Digest Journal of Nanomaterials and Biostructures, 447 451 (2011)
  20. Nagajyothi P. C., Lee K. D., Synthesis of plant-mediated silver nanoparticles using Dioscorea batatas rhizome extract and evaluation of their antimicrobial activities, Journal of Nanomaterials, doi:10.1155/2011/573429, (2011)
  21. Prashanth S., Menaka I., Muthezhilan R., Navin Kumar S., Synthesis of plant-mediated silver nanoparticles using medicinal plant extract and evaluation of its antimicrobial activities, International Journal of Engineering Science and Technology, 6235-6250 (2011)
  22. Brown T. Smith D., The effects of silver nitrate on the growth and ultrastructure of the yeast Cryptococcus albidus,Microbios Letter, 155-162 (1976)
  23. Richards R., Odelola M. E., Anderson B., Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomonas aeruginosa, Microbios, 39, 151-157 (1984)
  24. Zavriev S. K., Minchenkova L. E., Vorlickova M., Kolchinsky A. M., Volkenstein M. V., Ivanov V. I., Circular dichroism anisotropy of DNA with different modifications at N7 of guanine, Biochem. Biophys. Acta.564, 212-224 (1979)
  25. Lok C.N., Ho C.M., Chen R., He Q. Y., Yu W. Y., Sun H. Z., Tam P. K. H., Chiu J. F., Che C. M., Proteomic analysis of the mode of antibacterial action of silver nanoparticles, Journal of Proteome Research, 916-924 (2006)