Study Compare of Methods of Proportioning of the Reactive Protein C:Method of Agglutination and Method Immunoturbidimetrique at the Hospital of zone of Suru – Lere, Cotonou, Republic of Benin

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Study Compare of Methods of Proportioning of the Reactive Protein C:Method of Agglutination and Method Immunoturbidimetrique at the Hospital of zone of Suru – Lere, Cotonou, Republic of Benin

Author Affiliations

¹Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi – Université d’Abomey-Calavi, Cotonou, BENIN
² Laboratoire de biologie et de typage moléculaire en microbiologie, Département de biochimie et biologie cellulaire, Faculté des Sciences et techniques(FAST), Université d'Abomey-Calavi (UAC-Benin), Cotonou, BENIN

Int. Res. J. Biological Sci., Volume 2, Issue (3), Pages 85-87, March,10 (2013)

Abstract

On 135 Benin children 0 old to 14 years, of which 56% in the age bracket [0 - 5 years] were selected according to criteria's good determined for the comparison of two methods of proportioning of C Reactive Protein (CRP). The specimens were analyzed by immunoturbidimetry and agglutination with latex on plate. Uses of the two methods, the results obtained are as follows: on 135 samples 105 appeared positive ( 6mg/l) with 78% positive for the test of agglutination to latex and 81% for the test immunoturbidimetric. In the same way the test « t » of Student with P 0,05 revealed a significant difference between the averages of the two methods used.

References

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[ref1] Autier J., Miyara M. and Buyse S., Modulate 8: immunopathology, inflammatory reaction, item112. Issy-les-Moulineaux: Estem, 192 (2004)

[ref2] Cackle R., 250 tests of laboratory: regulation and interpretation, Paris: Elsevier Masson, 437 (2008)

[ref3] Vaishlavi L., Serum procalcitonin and Creactive protein levels ace markers off bacterial infection: systematic review and meta-analysis has, Repugnant covering joint, 39, 206-17 (1996)

[ref4] Santolaya M.E., Trunk J. and Bérési V., C-reactive protein: avaluable aid for the management off feverish children with cancer and neutropenia, Repugnant covering joint,18, 589-595 (2007)

[ref5] Bernad S., Clinical, Biochimy, Maloine: Paris (1985)

[ref6] Yassault A., Dumont G., Labbe Mr., Definition of the quality standards of a method of analysis: the monitor Internet, 26, 20-33 (1992)

[ref7] Eurachem Guides, The fitness for purpose off analytical method validation and related topics, Raven Near: New York, (1998)

[ref8] Yamashita K., Fujinaga T., Miyamoto T., Hagio Mr., Izumisawa Y., Kotani T., Relationship between serum cytokine activity and acute phase protein in human, The Medical newspaper off human Science, 56(3) 487-492 (2006)

[ref9] Collet B., Proteins of L-ignition, Thesis of Doctorate University, Claude Bernard, Lyon, 39-60 (1995)

[ref10] Medeci M.C., Mr. Martinelli, Albonetti V., Chezzi C. and Dettori G., Evaluation off rubella virus immunoglobulin G (IgG) and IgM assays vith of new Videa instrument. Newspaper off Clinical Microbiology, May, Flight 46 N°5, 1847-184 (2008)