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Detection of Immune Complexes in CSF of Tuberculous Meningitis cases in Correlation with Antibody Response

Author Affiliations

  • 1 Department of Medical Microbiology, University of Calicut, Kerala, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (3), Pages 69-75, March,10 (2013)

Abstract

A total of 800 CSF samples from patients suspected to TB meningitis were analyzed for anti-TB antibodies and mycobacterial immune complexes. About 24.375% of the cases showed anti-TB immune response by ELISA. Some CSF samples were positive only for anti-TB antibodies whereas some others were positive only for mycobacterial immune complexes. It appears that the immune complexes which are found before the free antibody could be detected. Immune complexes are a mixture of both antigen and antibodies. As the disease progresses the patient would have free antibodies and may become negative for immune complexes. It appears from the present study that the detection of both antibody and immune complexes is significant in the immunodiagnosis of the disease.

References

  1. Horner P.J. and Moss F.M., Tuberculosis in HIV infection, Int. J. STD AIDS, 162-167 (1991)
  2. Selwyn P.A., Tuberculosis in the AIDS era: A new threat from an old disease, NY State J. Med.,91, 233-235 (1991)
  3. Katti M.K., Assessment of antibody responses to antigens of Mycobacterium tuberculosis and cysticercus cellulosae in cerebrospinal fluid of chronic meningitis patients for definitive diagnosis as TBM/NCC by passive hemagglutination and immunoblot assays, FEMS Immunol. Med. Microbiol., 33, 57-61 (2002)
  4. Kashyap R.S., Agarwal N., Chandak N.C., Taori G.M., Biswas S.K. and Purohit H.J., Application of mancini technique as a diagnostic test in CSF of tuberculous meningitis patients, Med. Sci. Monit., 6, 95-98 (2002)
  5. Kennedy D.H. and Fallon R.J., Tuberculous meningitis, JAMA, 241, 264-268 (1979)
  6. Thwaites G., Chau T.T., Mai N.T., Drobniewski F. and McAdam K., Tuberculous meningitis, J. Neurol. Neurosurg. Psychiatry, 68, 289-299 (2000)
  7. Chakraborty N., Mukherjee A., Santra S., Sarkar R.N., Banerjee D., Guha S.K., Chakraborty S. and Bhattacharyya S.K., Current trends of opportunistic infections among HIV seropositive patients from Eastern India, Jpn. J. Infect. Dis.,, 49-53 (2008)
  8. Sooraj S.N., Nathiya K., Dhanabalan R., Angayarkanni J. and Palaniswamy M., Detection of mycobacterial antibodies in serum samples by enzyme linked immunosorbent assay, Afr. J. Biotechnol., 10, 16012-16015 2011) 9.Sooraj S.N., Nathiya K. and Palaniswamy M., Vaccine turned to diagnostic tool, Asian J. Pharm. Clin. Res., 54-56 (2012)
  9. Frieden T.R., Sterling T.R., Munsiff S.S., Watt C.J. and Dye C., Tuberculosis, Lancet, 362, 887–899 (2003)
  10. Grzybowski S., Barnett G.D. and Styblo K., Contacts of cases of active pulmonary tuberculosis, Bull Int. Union Tuberc.,50, 90-106 (1975)
  11. Lowell A.M., Tuberculosis: its social and economic impact and some thoughts on epidemiology. P. Kubica and L. G. Wayne (ed.), The mycobacteria. Part B. Marcel Dekker, New York., 1021-1056 (1984)
  12. Daniel T.M. and Debanne S.M., The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme linked immunosorbent assay, Am. Rev. Respir. Dis.,135,1137-1151 (1987)
  13. Van Vooren J.P., Turneer M., Yernault J.C., De Bruyn J., Burton E., Legros F. and Farber C.M., A multidot immunobinding assay for the serodiagnosis of tuberculosis, comparison with an enzyme linked immunosorbent assay, J. Immunol. Methods, 113, 45-49 (1988)
  14. Samuel A.M., Kadival G.V., Irani S., Pandya S.K. and Ganatra R.D., A sensitive and specific method for the diagnosis of tuberculous meningitis, Ind. J. Med. Res., 77, 752-757 (1983)
  15. Kalish S.B., Radin R.C., Leitz 0 Zeiss C.K. and Phair J.P., The enzymelinked immunosorbent assay method for IgG antibody to purified protein derivative in cerebrospinal fluid of patients with tuberculous meningitis, Ann. Int. Med., 99, 630 (1983)
  16. Chandramukhi A., Bothamley G.H., Brennan P.J. and Evanyi J., Levels of antibodies to defined antigens of Mycobacterium tuberculosis in tuberculous meningitis, J. Clin. Microbiol., 27, 821-825 (1989)
  17. Kadival G.V., Samuel A.M., Virdi S.S., Kale R.N. and Ganatra R.D., Radioimmunoassay of tuberculous antigen, Indian J. Med. Res.,75, 765-770 (1982)
  18. Samuel A.M., Kadival G.V., Ashtekar M.D. and Ganantra R.D., Evaluation of tubercular antigen and anti tubercular antibodies in pleural and ascitic effusions, Ind. J. Med. Res., 80, 563-565 (1984)
  19. Sada E., Ruiz-Palacios G.M., Lopezvidal Y. and Ponce De Leon S., Detection of mycobacterial antigens in CSF of patients with tuberculous meningitis by ELISA, Lancet, 651-652 (1984)
  20. Bhatia A.S., Kumar S. and Harinath B.C., Immunodiagnosis of Tuberculosis: An update, Ind. J. Clin. Biochem., 16(1), 132-135 (2001)