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Isolation, Partial purification and Characterization of Keratinase from Bacillus megaterium

Author Affiliations

  • 1 Dept. of Biochemistry, Center for Post Graduate Studies, Jain University, Jayanagar 3rd Block, Bangalore-560011, Karnataka, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (2), Pages 13-20, February,10 (2013)


A positive keratinase-producing bacterium from soil sample was screened and identified as Bacillus megaterium. The selected growth medium contained feather meal as a sole source of carbon and nitrogen at pH 10.0. The partially purified keratinase has a molecular mass of 41±1 kDa, optimum pH in the alkaline region (9.0) and optimum temperature of 50ºC. and Co2+ slightly inhibited the enzyme activity whereas Hg2+ exhibited a complete inhibition. NEM, IAA, TLCK and NAI moderately inhibited the enzyme whereas PMSF strongly inhibited the keratinase suggesting serine at the active site; however EDTA did not inhibit the enzyme. The isolated strain was thermotolerant and alkalophilic bacterium which can therefore be used in leather processing and detergent industry.


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