International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Bioautography guided Screening of Antimicrobial Compounds Produced by Microbispora V2

Author Affiliations

  • 1Department of Microbiology, PDEA’s Annasaheb Magar Mahavidyalaya, Hadapsar, Pune- 411028, MS, INDIA
  • 2 Department of Microbiology, University of Pune, Ganashkhind, Pune-411007, MS, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (2), Pages 65-38 and Bankar A.V., February,10 (2013)


The aim of this study was to investigate the antimicrobial activity of MicrobisporaV2. The genusMicrobispora is known to produce antimicrobial compounds like phenazines and others. The isolate Microbispora V2 can be exploited to mine out its capabilities of producing antimicrobial compounds utilizing cost effective production media. The screening is defined as the first step, which is applied to a sample, in order to establish the presence or absence of antimicrobial compounds. Bioautography belongs to microbiological screening methods commonly used for the detection of antimicrobial activity of a compound under study. Bioautography is a technique that combines thin Layer chromatography with bioassay in situ. It is one of the simplest and cheapest methods for detecting antimicrobial compounds in partially purified extracts because the method is easy to run, reproducible and requires less equipments. The fermentation process can be made cost effective by utilizing renewable energy sources like oil cake as sources of macronutrients of fermentation media. Therefore various oil cakes were chosen as nutrient source of fermentation media. Hence bioautography was used as a tool to screen out the production of antimicrobial compounds by Microbispora V2 employing oil cake fermentation media.


  1. Gerber N.N. and Lechevalier M.P. Phenazines & phenoxazinones from Waksmania aerata sp.nov. and Pseudomonas sp., Biochemistry, 3(4), 598–602 (1964)
  2. Gerber N.N. and Lechevalier M.P., 1, 6-Phenazinediol-5-oxide from Microorganisms, Biochemistry, 4(1), 176–180 (1965)
  3. Tanabe I., Kuriyama M. and Nonomura H., Production of C2 – symmetrical phenazines by some actinomycetes, Journal of fermentation and bioengineering, l79(4), 384-386 (1995)
  4. Cooper R., Horan A.C., Gunnarsson I., Patel M. and Truumees I., Nebularine from a novel Microbispora sp., Journal of Industrial Microbiology and Biotechnology., 19(4), 275-276 (1986)
  5. Igarashi Y., Takagi K., Kajiura T., Furumai T. and Oki T. Glucosyl questiomycin, a novel antibiotic from Microbispora sp. TP-A0184: fermentation, isolation, structure determination, synthesis and biological activities, J Antibiot (Tokyo), 51(10), 915-920 (1998)
  6. Patel M.G., Horan A.C., Marquez J.A. and Waitz J.A. Antifungal triacetylene dioxolone from Microbispora sp SCC1438 ATCC 53620, U.S. Patent 4, 956, 383 (1990)
  7. Ivanova V., Graefe U., Schlegel R., Schlegel B., Gusterova A., Kolarova M. and Aleksieva K., Isolation and structure elucidation of tyramine and indole alkaloids from antarctic strain Microbispora aerata imbas- 11A, Biotechnology and Biotechnological Equipments, 17(2), 128-133 (2003)
  8. Ivanova V., Kolarova M., Aleksieva K., Gräfe U., Dahse H.M. and Laatsch H., Microbiaeratin, a new natural indole alkaloid from a Microbispora aerata strain, isolated from Livingston Island, Antarctica. Prep Biochem Biotechnol,37(2), 161-168 (2007)
  9. Okujo N., Iinuma H., George A., Eim K.S., Li T.L., Ting N.S., Jye T.C., Hotta K., Hatsu M., Fukagawa Y., Shibahara S., Numata K. and Kondo S. Bispolides, novel 20-membered ring macrodiolide antibiotics from Microbispora. J. Antibiot., 60(3)216–219 (2007)
  10. Foulston L.C. and Bibb M.J., Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes, Proceedings of National Academy of Sciences of the United States of America, 107(30), 13461–13466 (2010)
  11. Burkhead K.D., Schisler D.A. and Slininger P.T., Bioautography shows antibiotic production by soil bacterial isolates antagonistic to fungal dry rot of potatoes, Soil Biology and Biochemistry,27(12)1611- 1616 (1995)
  12. Thomshow L.S., Bonsall R.F. and Weller D., Detection ofAntibiotics Produced by Soil and Rhizosphere Microbes In Situ. Book- Secondary Metabolites in Soil Ecology Book Series -Soil Biology, ISSN-1613- 3382 Volume 14 Publisher- Springer Berlin Heidelberg (2008)
  13. Choma I.M. and Grzelak E.M., Review- Bioautography detection in thin-layer chromatography, Journal of Chromatography A., 1218, 2684–2691 (2011)
  14. Hostettmann K., Strategy for the Biological and Chemical Evaluation of Plant Extracts, (1999)
  15. Goodall R.R. and Levi A.A., A microchromatographic method for the detection and approximate determination of the different penicillins in a mixture, Nature, 158, 675-676 (1946)
  16. Betina V., Bioautography in paper and thin-layer chromatography and its scope in the antibiotic field, J. Chromatography, 78, 41-51 (1973)
  17. Cos P., Vlietinck A.J., Berghe D.V. and Maes L., Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’, J. Ethnopharmacol., 106, 290-302 (2006)
  18. Rios J.L., Recio M.C. and Villar A., Screening methods for natural products with antimicrobial activity: A review of the literature, J. Ethnopharmacol,23, 127–149 (1988)
  19. Moreno M.I.N., Isla M.I., Cudmani N.G., Vattuone M.A. and Sampietro A.R., Screening of antibacterial activity of Amaicha del Valle (Tucumán, Argentina) propolis, J. Ethnopharmacol., 68, 97-102 (1999)
  20. Arun D. and Dharmalingam K., Streptomyces peuticusconverts anthracycline intermediates efficiently in culture media containing oil cake as carbon source, World Journal of Microbiology and Biotechnology,15(2), 333-334 (1999)
  21. Beattie S.H. and Williams A.G., Detection of Toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay, J. Appl. Microbiol., 82, 677-682 (1999)
  22. Rusul G. and Yaacob N.H., Prevalence of Bacillus cereusin selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA, Int. J. Food Microbiol., 25, 131-135 (1995)
  23. Kashid S.G. and Ghosh J.S., Production, Isolation and Characterization of Exotoxin Produced by Bacillus cereusNCIM-2156 and Bacillus licheniformis NCIM-5343, British Journal of Pharmacology and Toxicology,1(1), 50-55 (2010)
  24. Khond M., Bhosale J.D., Arif T., Mandal T.K., Padhi M.M. and Dabur R., Screening of Some Selected Medicinal Plants Extracts for In-vitro Antimicrobial Activity, Middle-East Journal of Scientific Research,4(4), 271-278 (2009)
  25. Punja Z.K., The biology, ecology and control of Sclerotium rolfsii, Annual Review of Phytopathology, 23, 97-127 (1985)
  26. Singh R.K. and Dwivedi R.S., Ecology and biology of Sclerotium rolfsii Sacc, International Journal of Tropical Plant Diseases,, 161-171 (1991)