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Comparison of the Therapeutic effects of Nano-essence of Medical herb Artemisia sieberi with the ointment of Ketoconazole in guinea pig infected by Microsporum canis

Author Affiliations

  • 1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, IRAN
  • 2 Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, IRAN

Int. Res. J. Biological Sci., Volume 2, Issue (12), Pages 5-10, December,10 (2013)

Abstract

Essences have well known properties. Transforming a drug to nano sized particles usually adds some additional potency to that drug. In this study we used Artemisia sieberi nano-essence to treat Microsporum canis induced dermatophytosis in guinea pig model. In vivo and In vitro methods were used to investigate the antifungal properties of nano-essence. Minimum inhibitory concentration of nano-essence was 0.3% to 2%. Treatment started 5 days after infection as 12 hours regimen until 45 days post infection. Both Ketoconazole and nano-essence groups had complete cure at day 40. Results show that this nano-essence is effective drug to treat M.canis induced dermatophytosis.

References

  1. Baldo A., Mondo M., Mathy A., L. Cambier, E.T. Bagut, V. Defaweux, F. Symoens, N. Antoine and B. Mignon, Mechanisms of skin adherence and invasion by dermatophytes, Myc, 55, 218-223 (2013)
  2. Fontenelle R., Morais S.M.,.Brito H.S.E, Brilhante R. S.N., Cordeiro R.A., Lima Y.C., Brasil N.V.G.P.S., Monteiro A.J.,.Sidrim J.J.C and Rocha M.F.G.., Alkylphenol Activity against Candida spp. and Microsporum canis: A focus on the antifungal activity of thymol, eugenol and O-Methyl Derivatives, Molecules, 16, 6422-6431 (2011)
  3. Scott D.W., Miller W.H. and Griffin C.E., Miller and Kirk’s Small Animal Dermatology, 1528 (2001)
  4. Medleau L. and Hnilica K., Small animal dermatology, 526 (2006)
  5. Lee S. and Han J., Antifungal effects of Eugenol and Nerolidol against Microsporum gypseum in a guinea pig model, Bio. and Pharma.Bul, 30, 184-188 (2007)
  6. Foster A. and Carol F., BSAVA manual of small animal dermatology, 2, 300 (2003)
  7. Rochette F., Engelen M. and Vanden Bosche H., Antifungal agents of use in animal health – practical applications, J Vet Pharmacol Ther 26, 31-53 (2003)
  8. Dash M., Chiellini F., Ottenbrite R.M. and Chiellini E., Chitosan—A versatile semi-synthetic polymer in biomedical applications, Progr.in Poly.Sci 36, 981-1014 (2011)
  9. Kong M., Chen X., Xing K. and Park H., Antimicrobial properties of chitosan and mode of action: A state of the art review, Inter.J.of Food Micro, 144, 51-63 (2010)
  10. Pillai C.K.S., Paul W. and Sharma C., Chitin and chitosan polymers: Chemistry, solubility and fiber formation, Prog. Polym. Sci., 34, 641-678 (2009)
  11. Sinha V.R. and Singla K.A., Chitosan microspheres as a potential carrier for drugs, Int. J Pharma, 274, 1-33 (2004)
  12. Siripatrawan U. and Harte B., Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract, Food Hydro, 24, 770-775 (2010)
  13. Abad M., Ansuategui M. and Bermejo P., Active antifungal substances from natural sources, ARKIVOC 2, 116-145 (2007)
  14. Abad M., Bedoya L., Apaza L. and Bermejo P., the Artemisia L. Genus: A Review of Bioactive Essential Oils,Molecules, 17, 2542-2566 (2012)
  15. Chen W., Vermaak I. and Viljoen A., Camphor—A Fumigant during the Black Death and a Coveted Fragrant Wood in Ancient Egypt and Babylon—A Review, Molecules, 18, 5434-5454 (2013)
  16. Chiasson H., Belanger A., Bostanian N., Vincent C. and Poliquin A., Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained from three methods of extraction, J. Econ. Entomol, 94, 167–171 (2001)
  17. Dehghani Bidgoli R. and Pessarakli M., Heshmati G.A., Comparison of Essential Oils in Aerial Parts of Artemisia Sieberi from Kashan Province of Iran, J. of Agri. Sci. and Tech, 14, 392-396 (2012)
  18. HAQ I., Mannan A., Ahmed I., Hussain I., Jamil M. and Mirza B., antibacterial activity and brine shrimp toxicity of Artemisia Dubai extract, Pak. J. of. Bot 44, 1487-1490 (2012)
  19. Liu Z., Chu S.S. and Liu R.,Chemical Composition and Insecticidal Activity against Sitophilus zeamais of the Essential Oils of Artemisia capillaries and Artemisia mongolica, Molecules, 15, 2600-2608 (2010)
  20. Mahboubi M., Iranian medicinal plants, J. of Micro.Biotech. And Food Sci 2, 2388-2405 (2013)
  21. Mahboubi M. and Farzin N., Antimicrobial activity of Artemisia sieberi essential oil from central Iran, Ira.J. of Microb, 43-48 (2009)
  22. Moghadamnia A. and Akhavan Tafi A., Evaluation of the effect of Artemisia Sieberi mouthwash 1% on denture stomatitis, Cas. J. of Med, 47-49 (2010)
  23. Monzote L., Alacron O. and Setzer W., Antiprotozoal Activity of Essential Oils, Agri.Cons. Sci, 77, 167-175 (2012)
  24. Pirbalouti G., Momeni A. and Bahmani M., Ethnobotanical study of medicinal plantsused by Kurd tribe in Dehloran and Abadan districts, Ilam province,2013, Afr J Tradit Complement Altern Med, 10, 368-385 (2013)
  25. Upadhyay R.K., essential oils antimicrobial antihelminthic antiviral anticancer and anti-insect properties, J. Appl. Biosci, 36, 1-22 (2010)
  26. Rodrigues C., Miranda K.C., Fernandes O.F.L., Soares A.J.and Silva M.R.R.,In vitro susceptibility testing of dermatophytes isolated in Goiania Brazil against five antifungal agents by broth microdilution method, Rev. Inst. Med. Trop. S. Paulo 51, 9-12 (2009)
  27. Singh J., Zaman M. and Gupta A.K., Evaluation of microdilution and disk diffusion methods for anti fungal susceptibility testing of dermatophytes, Mycoses, 45, 595-602 (2007)
  28. Ghannoum M.A., Long L., Cirino1 A.J., Miller A.R., Najafi R., Wang L., Sharma K., Anderson M. and Memarzadeh B., Efficacy of NVC-422 in the treatment of dermatophytosis caused byTrichophyton mentagrophytes using a guinea pig model, Inter. J.of derm, 52, 567–571 (2013)
  29. Neves CavalcantiI J., Guerra J. and Gamble W., Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis, Braz. J. vet. Res. anim. Sci., 39, 238-242 (2002)
  30. Shimamura T., Kubota N. and Shibuya K., AnimalModel of Dermatophytosis, J. of Biomed. and Biotech, 2012 1-11 (2012)