5th International Young Scientist Congress (IYSC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Isolation, Identification and Characterization of Curtobacterium sp. YU-SS-C-67 for phosphate Solubilization and Uranium Tolerance

Author Affiliations

  • 1 Yenepoya Research Center, Yenepoya University, Deralakatte, Mangalore – 575 018, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (12), Pages 102-106, December,10 (2013)

Abstract

Management of nuclear waste particularly uranium is of great environmental concern. Bioremediation of uranium using bacteria offers a less expensive, in situ alternative to the commonly used physico-chemical techniques. Recent bioremediation studies on heavy metals have focused on bioprecipitation as metal phosphates. In this respect, the present study deals with the isolation and characterization of a phosphate solubilizing Curtobacterium sp. YU-SS-C-67 from the vicinity of a proposed uranium mining site, Gogi (Karnataka, India). Following bacterial growth in the Pikovskaya’s broth, 271.13 mgL-1of phosphate was solubilized from insoluble tri-calcium phosphate with the drop in the media pH from 6.93 to 5.8. When tested for uranium sensitivity, the bacterium showed 12.89% reduction in cell number which was significantly lower (p 0.01) compared to 33.21% reduction seen in the reference strain Escherichia coli ATCC 25922. These results indicate that the isolate Curtobacterium sp. YU-SS-C-67 having the ability to solubilize phosphate as well as tolerate the chemical toxicity of uranium can find application in bioremediation technology. Further studies are demanded on isolation of microbial communities from these environments which may harbor interesting candidates for biological based remediation of uranium and other heavy metals.

References

  1. Wall J.D. and Krumholz L.R., Uranium reduction, Annu. Rev. Microbiol.,60, 149-166 (2006)
  2. Brugge D. and Buchner V., Health effects of uranium: new research findings, Rev. Environ. Health.,26, 231–249 (2011)
  3. Wu W.M., Carley J., Gentry T., Ginder-Vogel M.A., Fienen M., Mehlhorn T., Yan H., Caroll S., Pace M.N., Nyman J., Luo J., Gentile M.E., Fields M.W., Hickey R.F., Gu B., Watson D., Cirpka O.A., Zhou J., Fendorf S., Kitanidis P.K., Jardine P.M. and Criddle C.S., Pilot-scale in situ bioremediation of uranium in a highly contaminated aquifer. 2. Reduction of U(VI) and geochemical control of U(VI) bioavailability. Environ. Sci. Technol., 40, 3986–3995 (2006)
  4. Rejula F.A. and Dhinakaran M., Removal of zinc (II) by non living biomass of Agaricus Bisporus, Res. J. Recent Sci., 13-17 (2012)
  5. Gadd G.M., Metals, minerals and microbes: geomicrobiology and bioremediation, Microbiology,156, 609-643 (2010)
  6. Gavrilescu M., Pavel L. and Cretescu I., Characterization and remediation of soils contaminated with uranium, J. Hazard. Mater., 163, 475-510 (2009)
  7. Suzuki Y. and Banfield J.F., Resistance to, and accumulation of, uranium by bacteria from a uraniumcontaminated site, Geomicrobiol. J.,21, 113-121 (2004)
  8. Arey J.S., Seaman J.C. and Bertsch P.M., Immobilization of uranium in contaminated sediments by hydroxyapatite addition, Environ. Sci. Technol.,33, 337–342 (1999)
  9. Moon H.S., Komlos J. and Jaffé P.R., Uranium reoxidation in previously bioreduced sediment by dissolved oxygen and nitrate, Environ. Sci. Technol.,41, 4587–4592 (2007)
  10. Martinez R.J., Beazley M.J., Taillefert M., Arakaki A.K., Skolnick J. and Sobecky P.A., Aerobic uranium (VI) bioprecipitation by metal-resistant bacteria isolated from radionuclide-and metal-contaminated subsurface soils, Environ. Microbiol.,, 3122-3133 (2007)
  11. Hariprasad P. and Niranjana S.R., Isolation and characterization of phosphate solubilising rhizobacteria to improve plant health of tomato, Plant Soil, 316, 13-24 (2009)
  12. Madhavi V., Reddy A.V.B., Reddy K.G., Madhavi G. and Prasad T.N.V.K.V., An overview on research trends in remediation of chromium, Res. J. Recent Sci., 71-83 (2013)
  13. Pramila S., Fulekar M.H. and Bhawana P., E-waste – a challenge for tomorrow, Res. J. Recent Sci., 86-93 (2013)
  14. Pollmann K., Raff J., Merroun M., Fahmy K. and Selenska-Pobell S., Metal binding by bacteria from uranium mining waste piles and its technological applications, Biotech. Adv.,24, 58-68 (2006)
  15. Kumar P.G.N. and Bhat S.K., Fungal degradation of Azo dye – red 3BN and optimization of physic-chemical parameters, Int. Res. J. Biological Sci., 17-24 (2012)
  16. Vibha B. and Neelam G., Importance of exploration of microbial biodiversity, Int. Res. J. Biological Sci., 78-83 (2012)
  17. Pikovskaya R.I., Mobilization of phosphorus in soil in connection with vital activity of some microbial species, Microbiology,17, 362-370 (1948)
  18. Edi-Premono J., Moawad A.M. and Vlek P.L.G., Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere, Indones. J. Crop. Sci., 11,13–23 (1996)
  19. Chen P.S., Toribara T.Y. and Warner H., Microdetermination of phosphorous, Anal. Chem.,28,1756-1758 (1956)
  20. Tamura K., Dudley J., Nei M. and Kumar S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0, Mol. Biol. Evol.,24, 1596-1599 (2007)
  21. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. and Higgins D.G., The CLUSTAL_X windows interface: fexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic. Acids. Res.,25, 4876-4882 (1997)
  22. Chen Y.P., Rekha P.D., Arun A.B., Shen F.T., Lai W.A. and Young C.C., Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities, Appl. Soil. Ecol.,34, 33-41 (2006)
  23. Chabalala S. and Chirwa E.M.N., Removal of uranium (VI) under aerobic and anaerobic conditions using an indigenous mine consortium, Miner. Eng., 23, 526-531 (2010)
  24. Martins M., Faleiro M.L., Chaves S., Tenreiro R., Santos E and Costa M.C., Anaerobic bio-removal of uranium (VI) and chromium (VI): comparison of microbial community structure, J. Hazard. Mater.,176, 1065-1072 (2010)
  25. Macaskie L.E., Bonthrone K.M. and Rouch D.A., Phosphatase-mediated heavy metal accumulation by a Citrobacter sp. and related enterobacteria, FEMS Microbiol. Lett.,121,141-146 (1994)