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Isolation, Identification and Characterization of Curtobacterium sp. YU-SS-C-67 for phosphate Solubilization and Uranium Tolerance

Author Affiliations

  • 1 Yenepoya Research Center, Yenepoya University, Deralakatte, Mangalore – 575 018, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (12), Pages 102-106, December,10 (2013)

Abstract

Management of nuclear waste particularly uranium is of great environmental concern. Bioremediation of uranium using bacteria offers a less expensive, in situ alternative to the commonly used physico-chemical techniques. Recent bioremediation studies on heavy metals have focused on bioprecipitation as metal phosphates. In this respect, the present study deals with the isolation and characterization of a phosphate solubilizing Curtobacterium sp. YU-SS-C-67 from the vicinity of a proposed uranium mining site, Gogi (Karnataka, India). Following bacterial growth in the Pikovskaya’s broth, 271.13 mgL-1of phosphate was solubilized from insoluble tri-calcium phosphate with the drop in the media pH from 6.93 to 5.8. When tested for uranium sensitivity, the bacterium showed 12.89% reduction in cell number which was significantly lower (p 0.01) compared to 33.21% reduction seen in the reference strain Escherichia coli ATCC 25922. These results indicate that the isolate Curtobacterium sp. YU-SS-C-67 having the ability to solubilize phosphate as well as tolerate the chemical toxicity of uranium can find application in bioremediation technology. Further studies are demanded on isolation of microbial communities from these environments which may harbor interesting candidates for biological based remediation of uranium and other heavy metals.

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