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In Vitro Regeneration and Screening for Salt Tolerance in Rice (Oryza sativa L.)

Author Affiliations

  • 1Dept. of Genetic Engineering and Biotechnology, School of life sciences, Shahjalal University of Sci. and Tech., Sylhet-3114, BANGLADESH

Int. Res. J. Biological Sci., Volume 2, Issue (11), Pages 29-36, November,10 (2013)

Abstract

The study was conducted to obtain salt tolerant genotype of BRRI Dhan 38 and Chini Kanai (local variety) rice varieties through somaclonal variation. Different concentration and combinations of growth regulators were supplemented to MS medium to observe the callus induction and plantlet regeneration ability of mature rice seeds. On the other hand, the calli were transferred to the best regeneration medium at different concentrations of NaCl to check the inherent capacity of calli to regenerate on medium under salt stress condition. Maximum percentage of callus induction was observed in MS medium supplemented with 5 mg/l 2,4-D for BRRI Dhan 38 and 3 mg/l for Chini Kanai. Calli derived from the different concentrations of 2, 4-D were cultured on MS medium supplemented with 1 mg/l NAA, 2 mg/L BA and various concentration of Kinetin for plantlet regeneration. It was observed that MS media supplemented with 2 mg/l of kinetin in combination with 1 mg/l NAA and 2 mg/l BA produced highest percentage of callus for BRRI Dhan 38 (80%) and Chini Kanai (60%) respectively. Plant regeneration of BRRI dhan 38 was 80% at 0 mM NaCl, but decreased to 20% at 100 mM NaCl. There was 0% plant regeneration at 150 mM NaCl for BRRI 38 and Chini Kanai respectively. In Chini Kanai plant regeneration on the no-stress medium was 60%. At 150 mM it decreased to 20% and there was no regeneration at 200 mM NaCl. It indicates that Chini Kanai is more salt tolerant then BRRI Dhan 38.

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