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In Vitro Plantlets Regeneration of Terminalia bellirica Roxb. An important Medicinal Tree

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Author Affiliations

  • 1 PTC Lab, School of Life Sciences, Pt. Ravishankar Shukla University, Raipur – 492010, Chattisgarh, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (11), Pages 18-23, November,10 (2013)

Abstract

Rapid in vitro micropropagation protocol of T. bellirica was achieved using nodal explant from mature tree. Nodal explants when placed on Murashige and Skoog medium supplemented with 0.5 mgl-1 6-benzylaminopurine showed 100% shoot-bud with 4.5±0.56 shoot length per explant. The nodal segments from micro shoot obtain from induction medium were cultured on MS basal medium supplemented with different concentration of BAP and NAA, best shoot multiplication occurred with 0.25 mgl-1 BAP + 0.25 mgl-1 NAA. The shoot and node number increased significantly at third and fourth subculture of nodal segments. Excised shoots (2 cm - 3 cm long with 2 to 3 nodes) when grown on half MS basal medium with 0.25 mgl-1 indole-3-butyric acid (IBA) and pulse has shown rhizogenesis. The plantlets were washed to remove agar and then planted root trainers containing soil rite or sand soil mixture as substrate. These cultures were placed in green house for primary hardening. After four-weeks, plants were transferred from green house to net house where the plants exhibited gradual acclimatization to outdoor conditions.

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