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Isolation of lytic bacteriophage against Ralstonia solanacearum causing wilting symptoms in ginger (Zingiber officinale) and potato (Solanum tuberosum) plants

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Author Affiliations

  • 1 Research and Development Centre, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA
  • 2 Department of Biotechnology, IDSG Government College, Chikmagalur-577102, Karnataka, INDIA
  • 3 Department of Microbiology, School of Life Sciences, Karpagam University, Coimbatore-641021, Tamil Nadu, INDIA
  • 4 Department of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (11), Pages 78-84, November,10 (2013)

Abstract

This research work is aimed to find lytic activity of bacteriophage against phytopathogenic bacteria. Ralstonia solanacearum is a soil inhabiting bacterium colonizes in roots and tubers in various plants results in wilting in many vegetable crops especially in solanaceous plants. Ralstonia solanacearum invades in roots, stem and xylem vessels within the host and extensively multiplies in potato and ginger crops leads to economical losses. The bacteria were isolated from infected potato and ginger tubers. The isolates were characterized by TTC media and PCR based detection with Ralstonia solanacearum specific primers (F: 5’-GTCGCCGTCAACTCACTTTCC-3’, R: 5’-GTCGCCGTCAGCAATGCGGAATCG-3’). Use of bacteriophages for the control of plant bacterial diseases is fast expanding methodology over chemical aided control of bacteria. Therefore, we made attempt to isolate a lytic bacteriophage against Ralstonia solanacearum. The efficacy of phages as antimicrobial agents for specific bacteria is a relavant technology to overcome bacterial diseases without affecting the beneficial micro-flora of the soil. We could able to demonstrate the viable bacteriophages acts as omnilytics against specific bacteria. Bacteriophage HMPM-2012 was isolated against the phytopathogenic Ralstonia bacteria by soft agar diffusion method. In vitro confirmation of bacteriophage activity and electron microscopic studies were done. We are proposing phages as effective biocontrol agents in plant protection

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