International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Influence of Growth Media on Hydrphobicity of Phenol-Utilizing Bacteria Found in Petroleum Refinery Effluent

    ,

Author Affiliations

  • 1Department of Microbiology, Federal university of Technology, P.M.B.1526, Owerri, NIGERIA
  • 2 Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, NIGERIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 6-11, October,10 (2013)

Abstract

The effect of growth medium on hydrophobicity of phenol-utilizing bacteria isolated from petroleum refinery effluent was investigated. The hydrophobicity expression of the isolates were assessed via BATH, SAT and CRB assays. Four different growth media: tryptone soy broth (TSB), nutrient broth (NB), peptone water (PW) and Bushnell Haas broth (BH) was used. All the test isolates exhibited high to moderate hydrophobicity when grown on all the media. However, using SAT assay, Pseudomonas sp. RWW was found to be strongly hydrophobic (1.0) when grown in all the media while Escherichia sp. OPWW was found to be moderately hydrophobic (1.0 – 2.0) in all the media using the same SAT assay. Using Congo red binding assay, Bacillus sp. RBD was found to express weak hydrophobicity when grown in BH medium with Congo red up take value of 9.0 ΅g. All the media used for the assays were observed to be quite well for expression of hydrophobicity by refinery effluent bacteria in the following order: TSB &

References

  1. Ojumu T.V., Bello O.O., Sonibare J.A., Solomon B.O., Evaluation of microbial systems for bioremediation of petroleum refinery effluents in Nigeria, African Journal of Biotechnology,4(1), 31-35 (2005)
  2. 2.Wisjnuprapto J, Kardena E., Biotreatment of natural oil and gas industry wastes. Proceedings of the sixth AEESEAP Triennial Conference Kula, Bali Indonesia, August, pp, 23-25 (2000)
  3. Wake H., Oil refineries: a review of their ecological impacts on the aquatic environment, Estuary and Coastal Shelf Science,62, 131-140 (2005)
  4. Nwanyanwu C.E. and Abu G.O.,Assessment of viability responses of refinery effluent bacteria after exposure to phenol stress, Journal of Research in Biology,8, 594–602 (2011)
  5. Krishnakumar P.K., Dineshbabu A.P., Sasikummar G. and Bhat G.S.,Toxicity of treated refinery effluent using brine Shrimp (Artemia salina) egg and larval bioassay, Fishery Technology,44, 85–92 (2007)
  6. Walsh S.E., Maillard J.Y., Rusell A.D., Catrenich C.E., Charonneau D.L. and Bartolo RG.,Activity and mechanism of action of selected biocidal agents on gram positive and gram negative bacteria, Journal of Applied Microbiology,94, 240–247 (2003)
  7. Mafu A.A., Roy D., Savoie L. and Goulet J., Bioluminescence assay for estimating the hydrophobic properties of bacteria as revealed by hydrophobic interaction chromatography, Applied and Environmental Microbiology 57: 1640 – 1643, (1991)
  8. Noda Y. and Kanemasa Y., Determination of hydrophobicity on bacterial surfaces by nonionic surfactant, Journal of bacteriology, 167: 1016 – 1019, (1986)
  9. Fattom A., and Shilo M., Hydrophobicity as an adhesion mechanism of benthic cyanobacteria, Applied Environmental Micrbiology,47, 135–143 (1984)
  10. Cover W.H. and Rittenberg S.C., Changes in the surface hydrophobicity of substrate cells during bdelloplast formation by Bdellovibrio bacteriovorus 109, Journal of Bacteriology,157, 391–397 (1984)
  11. Vastsos I.N., Thompson K.D. and Adam A., Adhesion of the pathogen Flavobacterium psychrophilum to unfertilized eggs of rainbow trout (Oncorhynchus mykiss) and n-hexadecane, Letter of Applied Microbiology,33,178–182 (2001)
  12. Doyle R.J.,Contribution of the hydrophobic effect to microbial infection, Microbes and Infection,2, 391 400 (2000)
  13. Rosenberg M., Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity, FEMS Microbiology Letters,22, 289–295 (1984)
  14. Abu G.O. and Chikere B.O., Cell surface properties of hydrocarbon-utilizing bacterial isolates from the Port Harcourt marine environment, Nigerian Journal of Microbiology,20(1): 809 – 816, (2006)
  15. Jirku V., Masak J. and Cejkova A., Significance of physical attachment of fungi for bio-treatment of water, Microbiol. Res.,156: 383 – 386, (2001)
  16. Balebona M.C., Morinigo M.A. and Borrego J.J., Hydrophobicity and adhesion to fish cells and mucus of Vibrio strains isolated from infected fish, Int. Microbiol.,: 21 – 26, (2001)
  17. Heard J., Johnson B.B., Wells J.D. and Angove M.J.,Measuring ‘hydrophobicity’ of filamentous bacteria found in wastewater treatment plants, Colloidal and Surface B: Biointerface,72, 289–294 (2009)
  18. Daffonchio D., Thaveesri J. and Verstraete W., Contact angle measurement and cell hydrophobicity of granular of sludge from anaerobic sludge bed reactors, Applied and Environmental Microbiology 61: 3676 – 3680, (1995)
  19. Payne S.M. and Finkelstein R.A.,Detection and differentiation of iron-responsive avirulent mutants on Congo red agar, Infect. Immun.,18, 94–98 (1977)
  20. Nwanyanwu C.E., Alisi C.S., Nweke C.O., Orji J.C., Cell surface properties of phenol-utilizing bacteria isolated from petroleum refinery wastewater, Journal of Research in Biology, 2(4): 383 – 391, (2012)
  21. Basson A., Fleming L.A. and Chenia H.Y.,Evaluation of adherence, hydrophobicity, aggregation and biofilm development of Flavobacterium johnsoniae-like isolates, Microbiology Ecology,37, 1–14 (2008)
  22. Lindahl M., Faris A., Wadstrom T. and Hjerten S.,A new test based on salting out to measure relative surface hydrophobicity of bacterial cells, Biochim. Biophys. Acta., 677, 471–476 (1981)
  23. Majtan V. and Majtanova L., In vitro effect of fluoroquinolones and aminoglycosides on surface hydrophobicity and motility of Salmonella entericaserotype Typhimurium DT104, Biologia Brtislava,56(6): 625 – 631, (2001)
  24. Qadri F., S.A.Hossan, I.Ciznar, K.Haider, A.Ljungh, T.Wadstrom and D.A.Sack., Congo red binding and salt aggregation as indicator of virulence in Shigella species, J. Clin. Microbiol.,26, 1343 - 1348 (1988)
  25. Chrzanowski L., Bielicka-Daszkiewicz K., Owsianiak M., Aurich A., Kaczorek E. and Olszanowski A., Phenol and -alkanes (C12 and C16) utilization: influence on yeast cell surface hydrophobicity. World Journal of Microbiology and Biotechnology,24, 1943–1949 (2008)
  26. Sorongon M.L., Bloodgood R.A. and Burchard R.P.,Hydrophobicity, adhesion and surface-exposed proteins of gliding bacteria, Applied and Environmental Microbiology,57, 3193-3199 (1991)
  27. Lachica R.V. and Zink D.L., Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica, Infect. Immun, 44, 540–43 (1984)
  28. Prembrey R.S., Marshall K.C. and Schniedier R.P., Cell surface analysis techniques: What do cell preparation protocols do to cell surface properties, Applied and Environmental Microbiology,65, 2877-2894 (1999)
  29. Mamo W., Rozgonyi F., Brown A. and Hjerten H., Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase-negative Staphylococci from bovine mastitis, Journal of Applied Bacteriology,62, 241–249(1987)
  30. Jonsson P. and Wadstrom T., Cell surface hydrophobicity of Staphylococcus aureus measured by the salt aggregation test (SAT), Current Microbiology,10, 203–210 (1984)
  31. Das S.C.and Kapoor K.N., Effect of growth medium on hydrophobicity of Staphylococcus epidermidis. Indian J. Med. Res. 119: 107 – 109 (2004)
  32. Sanin S.L., Sanin F.D. and Bryers J.D., Effect of starvation on the adhesive properties of xenobiotic degrading bacteria, Process Biochemistry,38, 909-914 (2003)