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Decolourization of bromophenol blue and bromocresol green by laccase enzyme from Brassica oleraceae

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Author Affiliations

  • 1Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India
  • 2Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India
  • 3Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India
  • 4Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India

Int. Res. J. Biological Sci., Volume 10, Issue (4), Pages 24-28, November,10 (2021)

Abstract

Industrialization has led to a serious threat to water bodies and causing harmful impacts on living organisms. Industries discharge untreated waste water directly into the environment causing environmental pollution. Biotechnologists across the world are trying to develop innovative processes to check global pollution. However, the quantity of various pollutants discharged in the water bodies is increasing tremendously. One of the harmful pollutants is the synthetic dyes from textile industries. They are toxic and carcinogenic and are hazardous to human health. The present Research work focused on solving this problem cost-effectively to some extent. Laccase (E.C.1.10.3.2) is a significant and ubiquitous enzyme which plays avital role in degradation of synthetic dyes. In the current study, laccase enzyme from Brassica oleraceae has been isolated. The substrate used for laccase wasguaiacol. The enzyme activity was found to be 0.83U/ml. The kinetic parameters were studied and optimum conditions recorded were as follows: pH: 4, temperature: 100°C, incubation time: 30 minutes. The crude laccase enzyme was subjected to purification steps that involved ammonium sulphate salt precipitation followed by dialysis and anion-exchange chromatography using DEAE cellulose. The decolorization of synthetic dyes by laccase was seen after incubation of 72hrs. Complete degradation was accomplished in eight days.

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