International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Standardized protocol for the isolation of DNA from human saliva- a pilot study

    , , , ,

Author Affiliations

  • 1Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India
  • 2Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India
  • 3Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India
  • 4Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India
  • 5Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India

Int. Res. J. Biological Sci., Volume 10, Issue (3), Pages 40-44, August,10 (2021)

Abstract

This study aimed to standardize the methodology for the isolation of DNA from human saliva. The present study was conducted with 20 healthy control subjects with no history of smoking habits and other oral complications. The subjects were recruited from Chettinad Dental College and Research Institute (CDCRI) with the study protocol's approval from Institutional Human Ethics Committee (392/IHEC/10-17). Their age of the subjects was ranged between 30-45 years. Approximately 5 ml of human saliva was collected in a fresh tube with informed consent from healthy controls by informing the current research study by the donors. Genomic DNA was prepared from human saliva sample by ammonium acetate method. The quantification process was determined by a UV-visible spectrophotometer (UV-1800 Shimadzu spectrophotometer) to determine the purity and concentration of DNA. The average concentration of DNA from a saliva sample was found at1.48mg/ml.

References

  1. Saferstein, R. (2013)., Criminalistics., Pearson Education.
  2. Graham, M. H. (1986)., Expert Witness Testimony and the Federal Rules of Evidence: Ensuring Adequate Assurance of Trustworthiness., U. Ill. L. Rev., 43.
  3. Gefrides, L., & Welch, K. (2011)., Forensic biology: serology and DNA., In The forensic laboratory handbook procedures and practice, Humana Press. pp. 15-50.
  4. Graham, M. H. (1986)., Expert Witness Testimony and the Federal Rules of Evidence: Insuring Adequate Assurance of Trustworthiness., U. Ill. L. Rev., 43.
  5. Helmus, J., Poetsch, J., Pfeifer, M., Bajanowski, T., & Poetsch, M. (2020)., Cleaning a crime scene 2.0—what to do with the bloody knife after the crime?., International journal of legal medicine, 134(1), 171-175.
  6. Dash, H. R., Shrivastava, P., & Das, S. (2020)., Biological Samples: The Target Sources for DNA Typing. In Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing. Humana, New York, NY. pp. 13-20., undefined
  7. Kame, Introduction to this special issue: The DNA of reading fluency., Scientific studies of reading, 5(3), 203-210.
  8. Parker, G., Goecker, Z., Franklin, R., Durbin-Johnson, B., Milan, J., Karim, N., ... & Rice, B. (2019)., Proteomic genotyping: Using mass spectrometry to infer SNP genotypes in a forensic context., Forensic Science International: Genetics Supplement Series, 7(1), 664-666.
  9. Takamura, A., Halamkova, L., Ozawa, T., & Lednev, I. K. (2019)., Phenotype profiling for forensic purposes: determining donor sex based on Fourier transform infrared spectroscopy of urine traces., Analytical chemistry, 91(9), 6288-6295.
  10. Abel, S., & Schroeder, H. (2020)., From country marks to DNA markers: the genomic turn in the reconstruction of African identities., Current Anthropology, 61(S22), S198-S209.
  11. Yuan, L., Chen, X., Liu, Z., Liu, Q., Song, A., Bao, G., ... & Wu, Y. (2020)., Identification of the perpetrator among identical twins using next-generation sequencing technology: A case report., Forensic Science International: Genetics, 44, 102167.
  12. Balanovska, E., Lukianova, E., Kagazezheva, J., Maurer, A., Leybova, N., Agdzhoyan, A., ... & Balanovsky, O. (2020)., Optimizing the genetic prediction of the eye and hair color for North Eurasian populations., BMC genomics, 21(7), 1-13.
  13. Nema, V. (2020)., Utility and Possibility of Next-Generation Sequencing in Forensic DNA Typing., In Forensic DNA Typing: Principles, Applications and Advancements. Springer, Singapore. pp. 473-496.
  14. Dash, H. R., Shrivastava, P., & Das, S. (2020)., Collection, Transportation, and Preservation of Biological Evidences for DNA Analysis. In Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing., Humana, New York, NY. pp. 21-27.
  15. Dash, H. R., Shrivastava, P., & Das, S. (2020)., Isolation of DNA by Using Magnetic Bead-Based Extraction System. In Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing., Humana, New York, NY. pp. 87-96.
  16. Inci, F., Ozen, M. O., Saylan, Y., Miansari, M., Cimen, D., Dhara, R., ... & Demirci, U. (2018)., A novel on‐Chip method for differential extraction of sperm in forensic cases., Advanced Science, 5(9), 1800121.
  17. Hedman, J., Dalin, E., Rasmusson, B., & Ansell, R. (2011)., Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs., Forensic Science International: Genetics, 5(3), 194-198.
  18. Barbas, C. F., Burton, D. R., Scott, J. K., & Silverman, G. J. (2007)., Quantitation of DNA and RNA., Cold Spring Harbor Protocols, 2007(11), pdb-ip47.
  19. Tsai, L. C., Su, C. W., Lee, J. C. I., Lu, Y. S., Chen, H. C., Lin, Y. C., ... & Hsieh, H. M. (2018)., The detection and identification of saliva in forensic samples by RT-LAMP., Forensic Science, Medicine and Pathology, 14(4), 469-477.
  20. Garbieri, T. F., Brozoski, D. T., Dionisio, T. J., Santos, C. F., & NEVES, L. T. D. (2017)., Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols., Journal of Applied Oral Science, 25(2), 147-158.