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Evaluation of the cytotoxicity potential of ethanolic extract of Chrysophyllum cainitousing the brine shrimp lethality bioassay

Author Affiliations

  • 1Department of Marine Biology, Southern Philippines of Agri-Business and Marine and Aquatic School of Technology (SPAMAST), Poblacion Malita, Davao Occidental Philippines and College of Arts and Sciences, Xavier University-Ateneo De Cagayan, Cagayan de-Oro Philippines
  • 2Department of Marine Biology, Southern Philippines of Agri-Business and Marine and Aquatic School of Technology (SPAMAST), Poblacion Malita, Davao Occidental Philippines and College of Arts and Sciences, Xavier University-Ateneo De Cagayan, Cagayan de-Oro Philippines
  • 3Department of Biological Sciences, Mindanao State University-Iligan Institute of Technology (MSU-IIT), Tibanga, Iligan City Philippines and Department of Chemistry, Mindanao State University-Iligan Institute of Technology (MSU-IIT), Tibanga, Iligan City Philippines
  • 4Department of Biological Sciences, Mindanao State University-Iligan Institute of Technology (MSU-IIT), Tibanga, Iligan City Philippines and Department of Chemistry, Mindanao State University-Iligan Institute of Technology (MSU-IIT), Tibanga, Iligan City Philippines

Int. Res. J. Biological Sci., Volume 10, Issue (1), Pages 28-32, February,10 (2021)

Abstract

The Philippines has rich floral biodiversity accompanied with an abundant source of medicinal plants easily accessible in the locality. In terms of ethno-medical properties, Chrysophyllum cainito has been used to treat various diseases. In this study, C. cainito leaves were collected and evaluated for cytotoxicity using the Brine Shrimp Lethality Bioassay. The C. cainito leaves were extracted with water, 50:50 ethanol-water, and absolute ethanol to produce the decoction, hydro-ethanolic, and ethanolic extracts, respectively. Four concentrations (10, 100, 500, 1000&

References

  1. Boerma, T. Mathers,C. AbouZahr, C. Chatterji, S. Daniel Hogan, D. and Gretchen Stevens (2020)., Health in 2015 from Millenium Development goals (MDGS) to Sustainable Developmentgoals (SDGS)., https://apps.who. int/iris/bitstream/handle/10665/200009/9789241565110_eng.pdf;jsessionid=9B66AE28DA01F191520B528067B5DC10?sequence=1, June 2020.
  2. Debas H.T., Laxminarayan R. Straus SE. (2006)., Complementary and alternative medicine., Chapter 69. In: Jamison DT, Breman JG, Measham AR, et al., editors. Disease control priorities in developing countries. 2nd ed. Washington DC: World Bank, 2006.
  3. Ji, H.F. Li, X.J, Zhang H.Y. (2009).Natural products and drug discovery. EMBO Reports 10 (3), 194-200, undefined, undefined
  4. Doan, H.V, and Le T.P. (2020)., Chrysophyllum cainito: A Tropical Fruit with Multiple Health Benefits., Evidence-Based Complementary and Alternative Medicine 2020, 1-9. https://doi.org/10.1155/2020/7259267.
  5. Rymbai, H., Dutta, S.K., Jha, A.K., Deshmukh, N.A., Verma, V.K., War, G.G., Paul, D., Patel, R.S., Chaudri, P., Mishra, L.K., Roy, A.R. and Roy, D. (2015)., Chapter 32. Star Apple (Chrysophyllum cainito)., https://www. researchgate.net/publication/335240511_STAR_APPLE_Chrysophyllum_cainito/figures?lo=1&utm_source=google&utm_medium=organic, June 2020
  6. Luo, X.D., Basile, M.J. and Kennelly, E.J. (2002)., Polyphenolic antioxidants from the fruits of Chrysophyllum cainito (star apple)., J Agric Food Chem, 50(6), 1379-1382.
  7. Oranusi, S.U., Braide, W., Umeze, R.U. (2015)., Antimicrobial activities and chemical compositions of Chrysophyllum cainito (star apple) fruit., Microbiology Research International, 3(3), 41-50.
  8. Shailajan, S., and Gurjar, D. (2014)., Pharmacognostic and Phytochemical Evaluation of Chrysophyllum cainito Linnaeus Leaves., Int. J. Pharm. Sci. Rev. Res., 26(1), 106-111.
  9. Hegde, K., Arathi, A., and Mathew, A. (2016)., Evaluation of antidiabetic activity of hydro alcoholic extract of Chrysophyllum cainito fruits., International Journal of Pharmaceutical Sciences and Research, 7, 4422-4428.
  10. Da Rosa, R.L., De Almeida, C.L., Somensi, L.B., Boeing, T., Mariano, L.N.B., Krueger, C.M.A., De Souza, P., Filho, V.C., Da Silva, L.M., and De Andrade, S.F. (2019)., Chrysophyllum cainito (apple-star): A fruit with gastroprotective activity in experimental ulcer models., Inflammopharmacology 27(5), 985‐996. http://doi:10.1007 /s10787-017-0427-z
  11. Ernest, A. K., Marie-Solange, T., Beugre, K., & Noeuml, Z. G. (2009)., Effect of aqueous extract of Chrysophyllum cainito leaves on the glycaemia of diabetic rabbits., African Journal of Pharmacy and Pharmacology, 3(10), 501-506.
  12. Meira N.A., Klein, L.C., Rocha, L.W., Quintal, Z.M., Monache, F.D., Filho, V.C. and Quintão, N.L.M. (2014)., Anti-inflammatory and anti-hypersensitive effects of the crude extract, fractions and triterpenes obtained from Chrysophyllum cainito leaves in mice., Journal of Ethnopharmacology, 151(2), 975‐983. http://doi:10.1016/ j.jep.2013.12.014
  13. Shailajan, S., and Gurjar, D. (2016)., Wound healing activity of Chrysophyllum cainito L. leaves: evaluation in rats using excision wound model., Journal of Young Pharmacists, 8(2), 96-103.
  14. Houessou, L.G., Lougbegnon, T.O., Gbesso, F.G.H., Anagonou, L.E.S., Sinsin, B. (2012)., Ethno-botanical study of the African star apple (Chrysophyllum albidum G. Don) in the Southern Benin (West Africa)., Journal of Ethnobiology and Ethnomedicine, 8, 1-10
  15. Sarah, Q.S., Anny, F.C., and Misbahuddin, M. (2017)., Brine shrimp lethality assay., Bangladesh Journal Pharmacology, 12, 186-189.
  16. Meyer, N., Ferrigni, R., Putnam, E., Jacobsen, B., Nichols, E., and Mclaughlin, L. (1982)., Brine Shrimp: A convenient General Bioassay for Active Plant Constituents., Hippocrates Verlag Gmbh., 45, 31-34.
  17. Меntor, R., Jovanova, B. and Panovska, T.K. (2014)., Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model., Macedonian Pharmaceutical Bulletin, 60(1), 9-18.
  18. Olowa, L. and Nuneza, O. (2013)., Brine Shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants from Iligan City, Philippines., International Research Journal of Biological Sciences, 2(11), 74-77.
  19. Morilla, L.J., Nuneza, O. and Uy, M. (2015)., Brine Shrimp Lethality Test of Kleinhovia hospita stem and bark from Agusan del Sur, Philippines., ELBA Bioflux, 7(1).
  20. Elias, N., Nuneza, O. and Uy, M. (2014)., Evaluating the Potential Cytotoxic Activity of Acmella grandiflora Flower and Whole Plant using Brine Shrimp Lethality Test., International Research Journal of Biological Sciences, 3(10), 90-92.
  21. Arquion, C., Nuneza, O. and Uy, M. (2015)., Evaluating the Potential Cytotoxic Activity of Ficus nota Using Brine Shrimp Lethality Test., Bulletin of Environment, Pharmacology and Life Sciences, 4(12).
  22. Juario, J., Mondejar, E., Nuneza, O. and Uy, M. (2015)., Effect of Phyllanthus niruri and Passiflora foetida Extracts on the Mortality and Survival rate of the Brine Shrimp Artemia salina., Research Journal of Recent Sciences, 4(2), 61-67.
  23. Balinado, L. and Chan, M. (2017)., An Ethnomedicinal Study of Plants and Traditional Health Care Practices in District 7, Cavite, Philippines., International Conference on Chemical, Agricultural, Biological and Medical Sciences (CABMS-17).
  24. Chel-Guerrero, L., Sauri-Duch, E., Fragoso-Serrano, M.C., Perez-Flores, L.J., Gomez-Olivares, J.L,. Salinas-Arreortua, N., Sierra-Palacios, E., and Mendoza-Espinoza, JA. (2017)., Phytochemical Profile, Toxicity, and Pharmacological Potential of Peels from four Species of Tropical Fruits., Journal of Medicinal Food, 00(0), 1-10.
  25. Li, L.B., Lin, S., Yan, J., Wang, QL., Fan, Z.Y., Dong, Q.R., Qin, J.Z., and Xie, Z.G. (2015)., Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells., Bangladesh Journal of Pharmacology, 10, 972-979.
  26. Oputah, S., Mordi, R., Ajanaku, K., Olugbuyiro, J., Shade, J., Olorunshola, O., and Azuh, D. (2016)., Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)., Oriental Journal of Physical Sciences, 1(1 & 2), 05-09.
  27. Duyilemi, O.P., and Lawal, I.O. (2009)., Antibacterial activity and phytochemical screening of Chrysophyllum albidum leaves., Asian Journal of Food and Agro-Industry, S75-S79.
  28. Angone, S., Mewono, L., Boukandou, M.M., Medzegue, M.S., Ndong, J., Djoba Siawaya, J.F. and Souza, A. (2013)., Phytochemical screening and cytotoxicity studies of Chrysophyllum pruniforme Pierre ex Engl. Barks., Pharmacognosy Research, 5(3).