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Protective Effects of Vitamin C on Haematological Parameters in Intoxicated Wistar Rats with Cadmium, Mercury and Combined Cadmium and Mercury

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Author Affiliations

  • 1Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), Laboratory of Toxicology and Environmental Health, University of Abomey-Calavi (UAC), 01 BP 1463, Cotonou, BENIN
  • 2 Department of Geography and Planning, Processing Laboratory of Geographic Information and Planning Analysis, University of Abomey-Calavi (UAC), BP 939, Porto-Novo, BENIN
  • 3Department of Cell Biology and Genetics, Faculty of Science, university of Lagos, NIGERIA
  • 4Departement of Biochemistry and Cellular Biology, University of Abomey-Calavi (UAC), 01 BP 526, Cotonou, BENIN

Int. Res. J. Biological Sci., Volume 1, Issue (8), Pages 76-81, December,10 (2012)

Abstract

Cadmium and mercury are reported as heavy metals that induce blood disorders and immunological effects. This study was performed to determine the haematological toxicity of cadmium, mercury and evaluated the protective antioxidant role of vitamin C. For this purpose, 65 rats were divided into 13 lots of 5 animals, grouped into 2 groups of 6 lots and one control group. Two different doses of each metal and their combination were administered orally for 28 consecutive days to 6 lots of 1 group. The first two lots (A, B) respectively were exposed to cadmium chloride, two other lots (C, D) were respectively received mercury chloride and the last two lots of this group (E, F) were respectively treated with the combination of these two metals. The second group of 6 lots (Ac, Bc, Cc, Dc, Ec, Fc) respectively have received over the previous doses of metals, a daily dose of Vitamin C during the same period. The control group (G) received the same volume of distilled water. At the end of exposure, the body weight of rats was weighed and whole blood was collected by retro-orbital sinus for analysis of haematological parameters. The results of this study showed a significant decrease (P 0.05) on white blood cell, red blood cell, hemoglobin concentration, mean corpuscular, hemoglobin concentration with high concentrations of mercury and the combination of high concentrations of cadmium and mercury. However, co-administration of mercury, cadmium and mercury and vitamin C had a protective effect on the potential harmful metals.

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