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Impact of different Physical and Chemical Environment for mass production of Spirulina pletensis- An Immunity Promoter

Author Affiliations

  • 1 Algae Research Laboratory and Department of Biotechnology, Vital Biotech Research Institute, University of Kota, Kota, Rajasthan, INDIA
  • 2
  • 3
  • 4
  • 5
  • 6

Int. Res. J. Biological Sci., Volume 1, Issue (6), Pages 49-56, October,10 (2012)


Spirulina is one of the most explored cyanobacteria. Since ancient time it is being used as source of protein. Spirulina pletensis was cultivated in different medium like; zarrouk’s medium, CFTRI medium, OFERR medium, zarrouk’s medium + PGR medium, agitation and without agitation medium, improved VITAL BIOTECH medium. Different temperature, light intensity and pH were monitored for 20 days on daily basis. pH was found in range from 9.1 to 10.1in different medium. Gradually increase in dry weight (dw) was noticed along with the age of culture, 0.40-1.25 dw/l was achieved in different medium respectively. Spirulina inoculated in improved VITAL BIOTECH medium was survived and growth was flourished, achieving dry weight of 0.82 dw/l on 20th day of cultivation. Different amount of NaHCO and NaNO3 shown significant impact on Spirulina growth. However results of present investigation could be consider for commercial cultivation of Spirulina using different physical and chemical environment for mass production of Spirulina pletensis.


  1. Zhang X.W., Zhang Y.M. and Chen F., Process Biochem., 34, 477–481 (1999)
  2. Belay A., Mass culture of Spirulina outdoors–the Earthrise experience. In: Vonshak A (ed) Spirulina platensis (Arthrospira): physiology, cell-biology and biotechnology, Taylor and Francis, London, 131–158 (1997)
  3. Vonshak A., Spirulina platensis (Arthrospira), Physiology, Cellbiology and Biotechnology, Taylor and Francis, London (1997)
  4. Anupama P.R., Value-added food: single cell protein, Biotechnology Advances, 18, 459–479 (2000)
  5. Miranda M.S., Cintra R.G., Barros S.B.M. and Filho J.M., Antioxidant activity of the microalga Spirulina maxima,Brazilian J. of Medical and Bio. Res., 31, 1075–1079 (1998)
  6. Belay A., Ota Y., Miyakawa K. and Shimamatsu H., Current knowledge on potential health benefits of Spirulina, Journal of Applied Phycology, 5, 235–241 (1993)
  7. Estrada J.E., Bescós P. and Villar Del Fresno A.M.,Antioxidant activity of different fractions of Spirulina platensis protean extract, Farmaco, 56, 497–500 (2001)
  8. Torzillo G., Pushparaj B. and Bocci F., Production of Spirulina biomass in closed photobioreactors, Biomass., 11, 61-74 (1986)
  9. Mishra P.C., Dash A.K. and Pradhan Khageswar, Metals in Environmental segments at Hirakud of Odisha, India,ISCA J. Biological Sci., 1(1), 7-23 (2012)
  10. Khandkar-Siddikur Rahman, Nazmul Alam D.M. and Md. Nazrul Islam, Some Physical and Mechanical Properties of Bamboo Mat-Wood Veneer Plywood, ISCA J. Biological Sci., 1(2) 61-64 (2012)
  11. Singh A., Ahmed F. and Ahmed S., Bandey, ISCA J. Biological Sci., 1(2) 69-72 (2012)
  12. Patil J. and Patil H.M., Res. J. Recent Sci., 1(ISC-2011) 333-366 (2012)
  13. Shaziya Bi and Goyal P.K., ISCA J. Biological Sci.,1(1) 2-6 (2012)
  14. Sainkhediya Jeetendra and Aske Dilip Kumar, ISCA J. Biological Sci., 1(2) 77-79 (2012)
  15. Rani C.R., Reema C., Singh A. and Singh P.K., Salt tolerance of Sorghum bicolor cultivars during germination and seedling growth, Res J Recent Sci., 1(3) 1-10 (2012)
  16. Bhattacharya Anjanabha, Power John B. and Davey Micheal R.,Genetic Manipulation of Gibberellin (GA) Oxidase Genes in Nicotiana sylvestris using constitutive promoter to modify Plant Architecture, Res J Recent Sci.,1(5) 1-7 (2012)
  17. Eman A. Alam, ResJ Recent Sci.,1(ISC-2011) 398-404 (2012)
  18. Bora A., Science Commmunication through Mass media, Res J Recent Sci.,1(1) 10-15 (2012)
  19. Mehta J., Sain M., Sharma D.R., Gehlot P., Sharma P. and Dhaker J.K., I. Res. J. Biological Sci.,1(3), 37-42 (2012)
  20. Mehta J., Dhaker J.K., Kavia A., Sen P., Kaushal N., Datta S., I. Res. J. Biological Sci., 1(4), 12-16 (2012)