International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Evaluation of Antimicrobial and Anticancer activities of Methanol Extract of in vivo and in vitro grown Bauhinia variegata L.

Author Affiliations

  • 1* Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125, Noida 201301, INDIA
  • 2 Nano-Biotech Lab, Department of Zoology, K M College, University of Delhi, Delhi 110007, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (6), Pages 26-30, October,10 (2012)


The present investigation deals with the biological activities of the extracts of the medicinal plant Bauhinia variegata L.[BV], generated through in vivo and in vitro processes for their antibacterial and anticancer activities. Nodal explants of BV when placed on MS medium fortified with 6-Benzyl amino purine (BAP) at 5µg/ml resulted into multiple shoots. These shoots, on transfer developed bunch of roots in presence of Indole-3-butyric acid (IBA) at 2-4 µg/ml. The methanol extracts of such regenerated in vitro plants along with that of natural in vivo garden plants on comparison were found to be more effective against gram positive bacteria when compared to gram negative bacteria. But the screening of the in vitro cytotoxicity on EAC mouse cell lines responded almost with the same degree of inhibition for the ethanol extract, derived from both in vivo and in vitro sources.


  1. Anonymous, The Wealth of India, A Dictionary of Indian Raw Material and Industrial Products, Raw Material, Vol- 3: Ca-Ci, Revised Edition, Publication and Information Directorate, CSIR, New Delhi, 526-555 (2004)
  2. Ghaisis M.M., Shaikh S.A. and Deshpande A.D., Evaluation of immunomodulatory activity of ethanolic extract of stem bark of Bauhinia variegata Linn. International Journal of Green Pharmacy,70-74 (2009)
  3. Warrier P.K., Knambiar V.P. and Amankutty C.R., Indian Medicinal plants: A Compendium of 500 species, vol. 1. Madras, India: Orient Longman Limited, ISBN 0-86311464-4, (1993)
  4. Duret S. and Paris R.R., Plants of Nepal V. The flavonoids of various Bauhinia, B. valhit, B. variegate, B. malabarica; Leguminosa caesalpina. Planta Medica Phytologica et Therapeutica11, 213-21 (1977)
  5. Gupta A.K., Vidyapati T.S. and Chauhan J.S., Chemical examination of the stem of Bauhinia variegata, Planta Medica, 38, 174-76, (1979)
  6. Sahu G. and Gupta P.K., A review on Bauhinia variegata L. International Research Journal of Pharmacy, 3, 48-51 (2012)
  7. Khan Z.K., In vitro and in vivo screening techniques for antimicrobial and antifungal activity of medicinal plants, In: International workshop on Medicinal plants, their Biodiversity, screening and Evaluation. Lucknow, India: CDRI (1997)
  8. Pepeljnjak S., Stanic G. and Potocki P., Antimicrobial activity of the ethanolic extract of Satureja montana spp Montana,Acta Pharmaceutica, 19, 65-69 (1999)
  9. Vilegs J.H., DeMarchi E. and Laneas F.M., Extraction of low polarity compounds (with emphasis on coumarin and kaurenoic acids) from Mikania glomerata (guaco) leaves, Phytochem Anal, 266-70 (1997)
  10. Mathur J. and Mukunthakumar S., Micropropagation of Bauhinia variegata and Parkinsonia aculeate from nodal explants of mature trees, Plant, Cell, Tissue and Organ Culture,28, 119-21(1992)
  11. Pandey S. and Agarwal R.C., Effect of Bauhinia variegata bark extract on DMBA induced mouse skin carcinogenesis: A preliminary study, Global Journal of Pharmacology, 3(3), 158-62 (2009)
  12. Murashige T. and Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant, 15, 473-97 (1962)
  13. Mosmann T., Dornauer R. and Reinhard E., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunology Methods,65, 55-63 (1983)
  14. Upadhyaya R., Pandey R.P., Sharma V. and Verma A.K, Assessment of the multifaceted immunomodulatory potential of the aqueous extract of Tinospora cordifolia, Research Journal of Chemical Sciences,1(6), 1-7 (2011)
  15. Gholamerza Abbas S., Bioassay-Guided isolation and identification of an antibacterial compound from Ferula persica var persica roots, DARU, 13, 17-9 (2005)
  16. Parekh J., Nair R. and Chanda S., Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity, Indian Journal of Pharmacology, 37, 408-09 (2005)
  17. Frohne D., Plant intoxicants-is there a risk from herbal preparation? Journal of Phytotherapy, 20, 201-02 (1999)
  18. Romero C.D., Chopin S.F., Buck G., Martinez E., Garcia M., Garcia M. and Birby L., Antibacterial properties of common herbal of the southwest, Journal of Ethanopharmacology, 99,253-57 (2005)
  19. Rajkapoor B., Jayakar B., Murugesh N., Sakthisekaran D., Chemoprevention and cytotoxic effect of Bauhinia variegataagainst N-nitrosodiethylamine induced liver tumours and human cancer cell lines, Journal of Ethanopharmacology, 104, 407-09 (2006)
  20. Rajkapoor B., Jayakar B. and Murugesh N., Antitumour activity of Bauhinia variegata on Dalton’s ascetic lymphoma, Journal of Ethanopharmacology, 89, 107-09 (2003)
  21. Abeysinghe S., Preliminary in vitro screening of antibacterial compounds of some mangrove plant extracts of clinical isolated from different sources, Proceedings of the First Science Symposium, University of Ruhuna, 22-25, (2002)
  22. Chaitra H., Madhuri M., Nitisha S.T., Arijit D., Sourav B. and Rohit K.C., Evaluation of antimicrobial properties, phytochemical contents and antioxidant capacities of leaf extracts of Punica granatum L., ISCA Journal of Biological Sciences, 1(2), 32-37 (2012)