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In vitro Antioxidant Activity of Cassia tora Lin.

Author Affiliations

  • 1 Dept. of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Thiruvarur dt, Tamilnadu – 614001, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (6), Pages 57-61, October,10 (2012)


Free radicals are toxic byproducts of natural cell metabolism and are responsible for causing a wide number of health problems. The use and search of drugs and dietary supplements from plants with antioxidant activity are needed now a day. In this study methanolic leaves extract of Cassia tora was evaluated invitro by experimentral parameters such as DPPH scavenging activity, total antioxidant assay activity, scavenging superoxide anion radicals, nitric oxide radical, hydrogen peroxide scavenging capacity and total phenolic content. In the present study, reduction of the DPPH radicals was found in concentration- dependent manner. The results showed that Cassia tora displayed potent invitro antioxidant activity that may be attributed to the phenolics present in methanolic extract of the leaves of Cassia tora.


  1. Cowan M.M., Plant products as antimicrobial agents,Clinical microbial Analysis, 12, 64-582 (1999)
  2. Sies H., Oxidative Stress: Oxidants and Antioxidants, Academic Press, London, 276–280 (1991)
  3. Goldfarb A.H., Antioxidants: role of supplementation to prevent exercise-induced oxidative stress, Medicine and Science in Sports and Exercise, 25, 232-236 (1993)
  4. Halliwell B. and Gutteridge M.C., Free radicals in biology and medicine, 2nd Edition, (Charendon Press Oxford) (1999)
  5. Daiziel Afric J.M., The useful plants of West Tropical, London, 260–26 (1995)
  6. Rejiya C.S., Cibin T.R. and Abraham A., Leaves of Cassia tora as a novel cancer therapeutic-An in vitro study, Toxicology in vitro 23, 1034-1038 (2009)
  7. Chatterjee A. and Pakrashi S.C., The Treatise on Indian Medicinal Plants, 2, (Revised) CSIR: 45 (1992)
  8. Seth S.D. and Sharma B., Medicinal plants of India,Indian J.Med.Res.,120, 9-11 (2004)
  9. Rao A.R., Inhibitory action of Asparagus racemosus on DMBA- induced mammary carcinogenesis in rats, Int J Cancer28, 607-610 (1981)
  10. Maity T.K., Mandal S.C., Mukherjee P.K., Saha K., Das J., Pal M. and Saha B.P., Studies on anti-inflammatory effect of Cassia tora leaf extract (Fam. Leguminosae), Phytotherapy Research, 12(3), 221–223 (1999)
  11. Kalia A.N., Textbookof Industrial Pharmacognosy, CBS Publishers and Distributors, 1,(2009)
  12. Aleem M., Asif M., Ahmad Q.U., Bano B., Antidiabetic and antioxidant activity of Annona squamosa extract in streptozotocin induced diabetic rats, SingaporeMed J, 47, 670-5 (2006)
  13. Kaleem L., Sahin F., Gulluce M., Ogutcu H., Sngul M. and Adiguzel A., Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L, J Ethnopharmacol, 85, 231-235 (2006)
  14. Neube N.S., Assessment techniques of antimicrobial properties of natural Compounds of plant origin, Current methods and futre trends, .African J Biotchnol, 7, 1797-1806 (2008)
  15. I. Res. J. Biological Sci. International Science Congress Association 6115.Dodds J.H., The effect of phosphate, nitrogen and sucrose on acid From plants, Anal. Biochem, 53, 225-245 (1993)
  16. Barakat M.Z., Shehab S.K., Darwish N.E.I. and Zahermy, Determination of ascorbic the production of phenolics and solasidine in callus cultures of Solanum laciniatum,Plant Cell Reports, 2, 105-110 (1973)
  17. Shimada M.E., Gulcin I., Oktay M. and Kufrevioglu O.I., Invitro antioxidant properties of dantrolene sodium, Pharmacol. Res., 44, 491-95 (2001)
  18. Liu F., Ooi and Chang S.T., Free radical scavenging activity of mushroom, Journal Ethnopharmacology, 90(2-3), 249-252 (1997)
  19. Garrat D.C., Amelioration of chronic ileitis by nitric oxide synthetase inhibition, The J. Pharmac. and Experi., 3,456-458 (1999)
  20. Zhang X.Y., Principles of Chemical Analysis, Beijing: China Science Press, 275-276 (2000)
  21. Prieto A., Vila R., Freixa B., Adzet T. and Canigueral S., Antifungal activity of Paraguayan plants used in traditional medicine, J Ethnopharmacol, 76, 93-98 (1999)
  22. Chandler S.F. and Dodds J.H., The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum, Plant Cell Reports, 2, 105-110 (1993)
  23. Mokbel W. and Hashinaga B., The radical scavenging activities of radix puerariae isoflavonoids: A chemiluminescence study, Food Chem, 86, 525-529 (2005)
  24. Miller M.J., Sadowska-krowicka H., Chotinaruemol S., Kakkis J.L. and Miller A.L., Antioxidant flavonoids: Structure, function and clinical usage, Alt. Med. Rev., 1,103-111 (1993)
  25. Miller A.L., Antioxidant falvonoid; structure, function and clinical usage Linn - Compounds of plant origin, Current methods and futre trends, African J. Pharm., 64, 11-16 (1996)
  26. Okhawa N. Ohishi K. and Yagi H., Assay for lipid peroxides in animal tissues by thio-barbituric acid reaction, Anal. Biochem, 95, 351-358 (1979)