International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Production of cellulase free thermostable xylanase from Pseudomonas sp. XPB-6

Author Affiliations

  • 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (5), Pages 31-41, September,10 (2012)

Abstract

Pseudomonas sp. XPB-6 grows better in the medium containing xylan, glucose and meat extract, which was further modified by optimizing the concentrations of individual components. The organism was able to utilize most of the sugars for growth; however dextrose at 4% concentration proved to be the most suitable carbon source for production of xylanase activity from Pseudomonas sp. XPB-6. Different inorganic and organic nitrogen supplements were also tested and maximum production was achieved with 0.5% meat extract. The optimum temperature, pH substrate concentration and inoculum size for the growth and xylanase production were 30C, 7.0, 1% and 6% respectively. The optimum time for growth and enzyme production was 24 hours. Maximum xylanase activity was reported with 100 mM sodium phosphate buffer (pH 7.5) at 55C and 5 minutes incubation temperature. The enzyme was fairly stable at 25\rC and 75\rC. While studying the effect of various metal ions (addition of 1mM CuSO.5HO, CdCl.2HO, ZnSO.7HO and CaCl.2HO) drastic decrease in xylanase activity was observed.

References

  1. Butt M.S., Nadeem M.T., Ahmad Z. and Sultan M.T., Xylanases in Baking Industry, Food Technol. Biotechnol., , 22–31 (2008)
  2. Wong K.K.Y., Tan L.U.L. and Saddler J.N., Multiplicity of -1, 4-xylanase in microorganisms: Functions and applications, Microbiol. Rev.,52, 305–317 (1988)
  3. Poutanen K., Tenkanen M., Korte H. and Puls J., Accessory enzymes involved in the hydrolysis of xylans, In: G. F. Leatham and M. E. Himmel (Eds.), Enzymes in Biomass Conversion (pp. 426-436). American Chemical Society: Washington, DC (1991)
  4. Al-Bari M.A. A., Rahman M. M. S., Islam M. A. U., Flores M.E. and Bhuiyan M.S.A., Purification and Characterization of a -(1,4)-Endoxylanase of Streptomyces bangladeshiensis sp., Res. J. Cell Mol. Biol.,, 31-36 (2007)
  5. Kamble R.D. and Jadhav A.R.Isolation, purification and characterization of xylanase produced by a new species of Bacillusin solid state fermentation, Int. J. Microbiol., 8 (2012)
  6. Gilbert H.J. and Hazelwood G.P., Bacterial cellulases and xylanases, J. Gen. Microbiol.,139, 187-194 (1993)
  7. Knob A. and Carmona E.C., Xylanase production byPenicillium sclerotiorum and its characterization, W. Appl. Sci. Journal, 277-283 (2008)
  8. Beg Q. K., Kapoor M., Mahajan L. and Hoondal G. S., Microbial xylanases and their industrial applications: a review, Appl. Microbiol. . Biotechnol.,56(3-4), 326–338 (2001)
  9. Kuhad R.C., Kapoor M. and Chaudhary K., Production of xylanases from Streptomyces sp. M-83 using cost effective substrates and its application in improving digestibility of monogastric animal feed, Ind J Microbiol., 46 (2), 109-119 (2006)
  10. Polizeli M.L.T.M., Rizzatti A.C.S., Monti R., Terenzi H.F., Jorge J.S. and Amorim D.S.,Xylanases from fungi: Properties and industrial applications, Appl. Microb. Biotechnol.67, 577–591 (2005)
  11. Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, 538–542 (1959)
  12. Poorma C.A. and Prema P., Production of cellulase free endoxylanase from novel alkalophilic thermotolerant Bacillus pumilus by solid state fermentation and its application in waste paper recycling, Biores.Technol., 98, 485-490 (2007)
  13. Inan K., Belduz A.O. and Canakci S., Anoxybacillus kaynarcensis sp. a moderately thermophilic, xylanase producing bacterium, J. Basic Microbiol., 52, 1–10 (2012)
  14. Park Y.K., Yum D.Y., Bal D.H. and Yu J.H., Xylanase from alkalophillicBacillus sp. YC- 335, Bios. Biotechnol. Bioeng., 56, 1355-1356 (1992)
  15. Nakamura S., Wakabayashi K., Nakai R., Aono R. and Horikoshi K., Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1, W. J. Microbiol. Biotechnol., 59(7), 2311–2316 (1993)
  16. Yang W.V., Zhuang Z., Eligir G. and Jeffries T.W., Alkalineactivexylanase produced by an alkaliphilic Bacillus sp isolated from kraft pulp, J. Ind .Microbiol., 15(5), 434–441 (1995)
  17. Kheng P.P. and Omar I.C., Xylanase production by a local fungal isolate Apergillus niger USM AI 1 via solidstate fermentation using palm kernel cake (PKC) as substrate Songklanakarin, Song. J. Sci. Technol., 27, 325–336 (2005)
  18. Ghoshal G., Banerjee U.C., Chisti Y. and Shivharea U.S., Optimization of xylanase production from Penicillium citrinum in solid-state fermentation, Chem. Biochem. Eng. Q., 26 (1), 61–69 (2012)
  19. Bakri Y., Jawhar M. and Arabi M. I. E., Improvement of xylanase production by Cochliobolus sativus in submerged culture, Food Technol Biotechnol.,46, 116-118 (2008)
  20. Ghosh M., Das A., Mishra A.K. and Nanda G., Aspergillus sydowii MG 49 is a strong producer of thermostable xylanolytic enzymes, Enz. Microb. Technol., 15, 703-779 (1993)
  21. Haltrich D., Nidetzky B., Kulbe K.D., Steiner W. and Zupancic S.Production of fungal xylanases, Biores. Technol.,58, 137-161 (1996)
  22. Laxmi G.S., Sathish T., Rao S., Brahmaiah P., Hymavathi M. and Prakasham R.S., Palm fiber as novel substrate for enhanced xylanase production by isolated Aspergillus sp. RSP-6, Curr. Tren. Biotechnol. Pharm., 2(3), 447-455 (2008)
  23. Muthezhilan R., Ashok R., and Jayalakshmi S., Productionand optimization of thermostable alkaline xylanase by Pencilliumoxalicum in solid-state fermentation, Afr. J. Microbiol. Res., 20–28 (2007)
  24. Sanghi A. Garg N., Gupta V.K., Mittal A.K. and Kuhad R.C.,One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis, ASH Braz. J. Microbiol., 41, 467-476 (2010)
  25. Purkarthofer H., Sinner M. and Steiner W., Cellulase-free xylanase from Thermomyces lanuginosus: Optimization of production in submerged and solid state culture, Enz. Microb. Technol., 15, 677-682 (1993)
  26. Dhillon A. and Khanna S., Production of a thermostable alkalitolerant xylanase from Bacillus circulans AB16 grown on wheat straw, W. J. Microbiol. Biotechnol., 27, 325–327 (2000)
  27. Shah A. R. and Madamwar D., Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization, Proc. Biochem.,40, 1763-1771 (2005)
  28. Khasin A., Alchanati I. and Shoham Y., Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6, Appl. Environ. Microbiol., 13, 365 (1993)
  29. Sīa-Pereira P., Costa-Ferreira M. and Aires-Barros M. R., Enzymatic properties of a neutral endo-1,3(4)--xylanase XylII from Bacillus subtilis, J. Biotechnol., 94(3), 256–275 (2002)