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Production of cellulase free thermostable xylanase from Pseudomonas sp. XPB-6

Author Affiliations

  • 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (5), Pages 31-41, September,10 (2012)

Abstract

Pseudomonas sp. XPB-6 grows better in the medium containing xylan, glucose and meat extract, which was further modified by optimizing the concentrations of individual components. The organism was able to utilize most of the sugars for growth; however dextrose at 4% concentration proved to be the most suitable carbon source for production of xylanase activity from Pseudomonas sp. XPB-6. Different inorganic and organic nitrogen supplements were also tested and maximum production was achieved with 0.5% meat extract. The optimum temperature, pH substrate concentration and inoculum size for the growth and xylanase production were 30C, 7.0, 1% and 6% respectively. The optimum time for growth and enzyme production was 24 hours. Maximum xylanase activity was reported with 100 mM sodium phosphate buffer (pH 7.5) at 55C and 5 minutes incubation temperature. The enzyme was fairly stable at 25\rC and 75\rC. While studying the effect of various metal ions (addition of 1mM CuSO.5HO, CdCl.2HO, ZnSO.7HO and CaCl.2HO) drastic decrease in xylanase activity was observed.

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