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In-vitro Antimicrobial and Cytotoxic ativity of Methanolic extract of Osbeckia wynaadensis

Author Affiliations

  • 1* Department of Chemistry, Karpagam University, Coimbatore-641 021, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (4), Pages 33-38, August,10 (2012)

Abstract

In- vitro antibacterial and antifungal activities of methanolic extract of osbeckia wynaadensis was evaluated in the present study for the first time by disc diffusion method using five bacterial strains (S. pneumonia, B.cereus, A. hydrophila , V. cholera and MRSA) and five fungal strains (Candida albicans, Aspergillus niger, Streptomysis Greusis, M. purpureus and Aspergillus fumigate) respectively. The zone of inhibition and minimum inhibitory concentration were measured. Ampicillin (30 g/disc) and clotrimazole (20 g/disc) were used as standard for antibacterial and antifungal activity respectively. In-vitro cytotoxic activity of Osbeckia wynaadensis was evaluated against the human cervical adenocarcinoma cell line (HeLa) and murine embryonic fibroblasts cell line (NIH 3T3) by MTT assay and the IC50 value found to be 220.3 g/mL and 93. 25 g/mL. It is concluded that Osbeckia wynaadensis exhibited significant antibacterial, antifungal & in-vitro cytotoxic activity.

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