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Survey on Drug Resistant Pattern of Clinical Isolates and Effect of Plant Extract on the Drug Resistant Pattern

Author Affiliations

  • 1Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA
  • 2Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (3), Pages 14-19, July,10 (2012)

Abstract

Drug resistance is an emerging fact of the human beings leading to an increase in mortality rate. For this instance clinical samples were collected such as urine, pus, sputum, and catheter samples from private clinical laboratories, Madurai. The following organisms were isolated and characterized as E. coli (49/111), Klebsiella pneumonia (28/111), proteus mirabilis (8/111), pseudomonas aeruginosa (18/111) and staphylococcus aureus (8/111). Further these clinical isolates were subjected to SDS treatment or pomegranate extract treatment for plasmid curing and continued with antibiotic susceptibility test. In our present study, ceftriaxone was found to be more active than any other antibiotics. On the contrary, penicillin G was found to be inactive against all the clinical isolates. The plasmid DNA was separated by agarose gel electrophoresis in E. coli and pseudomonas aeruginosa. Thus resistant organisms, which was examined as multidrug resistance was taken into account for molecular characterization of gene responsible. The plasmids were cured in all the isolates by sodium dodeyl sulphate (or) pomegranate extract. There was no correlation between plasmid pattern and their antibiogram.

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