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Anthelmintic effect of Natural Plant (Carica papaya) extract against the Gastrointestinal nematode, Ancylostoma caninum in Mice

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Author Affiliations

  • 1 School of Studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (1), Pages 2-6, May,10 (2012)

Abstract

Infection with gastrointestinal nematode have severe consequences for the health of millions of people worldwide, and cause serious economic losses in live stock farming. Synthetic drug have been considered the most effective way of controlling parasite infections. But these drugs are expensive and sometime unavailable to people and show the side effect hence anthelmintic offer a simple, cheap, cost effective method of controlling parasites with no side effect. The purpose of this experiment was to study the anthelmintic activity of Carica papaya extract against Ancylostoma caninum in infection in mice. Two experiments were setup for this study, in experiment no. 1, two groups (A and B) and experiment no. 2, three groups (A, B and C) of mice were taken for larval recovery and mast cell & eosinophil counts respectively. Group A mice were treated with plant extract (Carica papaya) 0.2 ml/ mouse, on day -14 and -7 day before challenge infection and on day 0 mice were challenge with 500 A. caninum larvae. Group B mice were challenge only with dose of 500 Ancylostoma caninum larvae. Group C served as a non treated control. Results of plant extract treated mice clearly demonstrated a reduction of larvae in group (A) when compared with group (B) of mice. Large number of mucosal mast cell observed on day 16 in all groups. Eosinophil levels were markedly reduced in 24 days after challenge infection in all groups. The results suggest a potential role of Carica papaya extract as an anthelmintic activity against intestinal nematodes infection.

References

  1. Perry B. D. and Randolph T. F., Improving the assessment of the economic impact of parasitic diseases and of this control in production animals. Veterinary Parasitology, 84, 145 – 168 (1999)
  2. Saeed M., Iqbal Z. and Jabbar A., Oxfendazole resistance in gastrointestinal nematodes of beetal goats at livestock farms of Punjab (Pakistan). Acta Veterinaria Brno, 76, 79 – 85 (2007)
  3. Jabbar A., Iqbal Z., Kerboeuf D., Muhammad G., Khan M. N. and Afaq M., Anthelmintic resistance the state of play revisited. Life Science, 79, 2413 – 2431 (2006)
  4. Saddiqi H. A., Jabbar A., Iqbal Z., Babar W., Sindhu Z. D. and Abbas R. Z., Comparative efficacy of five anthelmintics against trichostrongylid nematodes in sheep. Canadian Journal of Animal Science, 86, 471 – 477 (2006)
  5. Waller P. J., Echevarria F., Eddi C., Maciel S., Naria A. and Hansen J. W., The prevalence of anthelmintic resistance in nematode parasites of sheep in Southern Latin America, general overview, Veterinary Parasitology, 62, 181 – 187 (1996)
  6. Praslicka J., Some aspects of Spread of anthelmintic resistance. Helminthology, 32, 75 – 77 (1995)
  7. Berger J. and Asenjo C. F., Anthelmintic activity of fresh pineapple juice. Science, 90, 299 – 300 (1939)
  8. Robbins B. H., A proteolytic enzyme in ficin, the anthelmintic principal of Leche de Higueron. Journal of Biological Chemistry, 87, 251 – 527 (1930)
  9. Wilkinson W. C. and Behnke J. M., Necator americanus in the mouse, histopathological changes associated with the passage of larvae through the lungs of nice exposed to primary and secondary infection. Parasitology Research, 76, 386 – 392 (1990)
  10. Berger J. and Asenjo C. F., Anthelmintic activity of crystalline papin. Science, 91, 387 – 388 (1940)
  11. Sen K. G., Joshi U. N. and Seth D., Effect of cortisone upon Ancylostoma caninum infection in albino mice. Trans. Roy. Soc. Trop. Med. Hyg; 59, 684 – 689 (1965)
  12. Scott J. A., An experimental study of the development of Ancylostoma caninum in normal and abnormal hosts. Amer. J. Hyg; 8, 158 – 209 (1928)
  13. Bruning J. L. and Kintz B. L., Computational hand book of statistics, II ed., Scott, Foresman, California, Glen View I. Ltd (1977)
  14. Mursof E. P. and He S., A potential role of papaya latex as an anthelmintic against patent Ascaridia galli infection in chicken. Hemera Zoa, 74, 11 – 20 (1991)
  15. Dakpogan H. B., Free range chick survivability in improved conditions and the effects of three medicinal plants on Eiemeria tenella. M. Sc. Thesis Department of Veterinary Pathobiology, The Royal Veterinary University, Denmark (2005)