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Morphological and morphometrical analyses of Blood Cells of Emus (Dromaius novaehollandiae) in Captive condition

Author Affiliations

  • 1State Forensic Science Laboratory, Bhubaneswar, Odisha, India

Res. J. Forensic Sci., Volume 8, Issue (2), Pages 1-8, July,29 (2020)

Abstract

The designed of the study was made for analyses of morphology and morphometry (i.e., measurement) of cells of blood of emus in captive condition. Since studies on morphological features of erythrocytes and leucocytes of captive emus were not properly documented in literature, to know the various shapes of erythrocytes (RBCs) and leucocytes of them and to study morphometry of blood cells in four different months this work is undertaken. For observance of cellular morphology of erythrocytes and leukocytes in them total fourteen emus were taken for the present study. From the jugular veins sample blood were taken by an expert from one private farm of Bhubaneswar, Odisha, India and transferred into EDTA vials and blood smears were made on clean microscopic slides. Then staining of blood smears were done with Giemsa stain to study the cellular morphology. The findings of the study reveal that captive emus have various shapes of erythrocytes and by this type of study shapes of normal erythrocyte and abnormal erythrocytes can be known. RBCs and their nuclei and leucocytes were photomicrographed and observed below the microscope under 40X objective. Length and breadth of RBCs and their nuclei, lymphocytes and their nuclei and other leucocytes were measured with the help of ocular micrometer and stage micrometer. Mean and standard error were calculated for statistical analyses.

References

  1. Yoganathan, S., Nicolosi, R., Wilson, T., Handelman, G., Scollin, P., Tao, R., Binford, P., & Orthoefer, F. (2003)., Anagonism of croton oil inflammation by topical emu oil in CD-1 mice., Lipids, 38, 603-607.
  2. Qiu, X.W., Wang, J.H., Fang, X.W., Gong, Z.Y., Li, Z.Q., & Yi, Z.H. (2005)., Anti-inflammatory activity and healing-promoting effects of topical application of emu oil on wounds in scalded rats., Acad. J. First Med. Coll. P.L.A., 25, 407-410.
  3. Howarth, G.S., Lindsay, R.J., Butler, R.N., &Geier, M.S. (2008)., Can emu oil ameliorate inflammatory disorders affecting the gastrointestinal system?., Aust. J. Exp. Agric., 48, 1276-1279.
  4. Lindsay, R.J., Geier, M.S., Yazbeck, R., Butler, R.N., & Howarth, G.S. (2010)., Orally administered emu oil decreases acute inflammation and alters selected small intestinal parameters in a rat model of mucositis., Br. J. Nutr., 104, 513-519.
  5. Abimosleh, S., Lindsay, R.J., Butler, R.N., Cummins, A.G., & Howarth, G.S. (2012)., Emu oil increases colonic crypt depth in a rat model of ulcerative colitis., Dig. Dis. Sci., 57, 887-896.
  6. Zemstov, A., Gaddis, M., & Montalvo-Lugo, V.M. (1996)., Moisturizing and cosmetic properties of emu oil: a pilot double blind study., Australas. J. Dermatol., 37, 159-161.
  7. Tully, T.N., & Shane, S.M. (1996b)., Husbandry practices as related to infections and parasitic diseases of farmed ratites., Rev. Sci. Tech. Paris., 15, 73-89.
  8. Mohanty, P.K., Puspamitra, S., Dash, I., Kullu, S.R., Bhattacherjee, A., & Nayak, S (2017)., Season wise haematological parameters of flightless bird emu Dromaiusnovaehollandiae (Lantham,1790)., I.J.B., 4(2), 137-141.
  9. Harvey, J.W. (2008)., The erythrocyte: physiology metabolism and biochemical disorders., In : Kareko, J.J., Harvey, J.W., & Bruss, M.L. (eds). Clinical Biochemistry of Domestic animals, 6th edn., San Diego: Academic Press, USA , pp 173-240.
  10. Harvey, J.W. (2010)., Schalm’s Veterinary Hematology., In: Weiss, D.J., & Wardrop, K.J. (eds). Erythrocyte biochemistry, 6th edn., Wiley-Blackwell Publishing Ltd., Ames. USA, Iowa, pp 131-135.
  11. Thrall, M. A., Weiser, G., Allison, R., & Campbell, T. (Eds.). (2012)., Veterinary hematology and clinical chemistry., John Wiley & Sons., Publication, USA, pp 63.
  12. Lillie, R.D. (ed.) (1997)., Conn’s HJ biological stains., 9thedn. The Williams and Wilkins Company. Baltimore, USA, pp 606-607.
  13. Dash, I., & Mohanty, P.K. (2015)., Morphological features and influence of age and breed on the morphometry of red blood cells of female cattle., I.J.S.R., 4(2), 259-264.
  14. Lumeij, J.T., (1997)., Avian clinical biochemistry. In Clinical biochemistry of domestic animals., eds. Kaneko, J.J., Harvey, J.W., Bruss, M.L., 5th edn. San Diego: Academic Press, pp. 932.
  15. Fudge, A.M. (1997)., Avian clinical pathology–hematology and chemistry., In: Altman, R.B., Club, S.L., Dorrestein, G.M., & Quesenberry, K. (eds). Avian medicine and surgery Philadelphia: WB Saunders, pp 142-157.
  16. Bhattacherjee, A., Mohanty, P.K. and Mallik, B.K. (2014)., Morphometrical analysis of blood cells of Japanese quail (Coturnixcoturnix japonica) in two different age groups., Int. Res. J. Biological. Sci., 3(9), 38-41.
  17. Hartman, F. A., & Lessler, M. A. (1964)., Erythrocytes measurements in fishes, amphibian and reptiles., The. Biol. Bull., 126(1), 83-88.
  18. Tadjalli, M., Nazifi, S., Abbasabadi, B.M., & Majidi, B. (2013)., Histomorphometric study on blood cells in male adult ostrich., Vet. Res. Forum., 4(3), 199-203.
  19. Lucas, A. M., & Jamroz, C. (1961)., Atlas of Avian Haematology., Agriculture Monograph 25. United States. Department of Agriculture, Washington, pp 214-221.
  20. Bounous, D.I., & Stedman N.L. (2000)., Normal avian hematology: chicken and turkey., In: Feldman, B.V., Zinkl, J.G., & Jain, N.C (eds). Schalm’s veterinary hematology, Philadelphia: Williams & Wilkins, pp 1145-1154.
  21. Cardoso, A.L.S.P., & Tessari, E.N.C. (2003)., Estudo do parâmetros hematológicos em frangos de corte., Arquivo. do. Instituto. De. Biologia., 70(4), 419-424.
  22. Campbell, T.W. (2012)., Hematology of birds., In: Thrall, M.A., Weiser, G., Allison, R.W., & Campbell, T (eds). Veterinary Haematology and Clinical Chemistry, 2nd edn. Wiley– Blackwell A John Wiley and Sons, Inc., Publication, USA, pp 242.
  23. Blue –McLendon, A. and Green, R.A., (2010)., Hematology of ratites., In Schalm’s veterinary hematology, eds. Weiss, D.J., Wardrop, K.J., 6th ed. Ames: Lippincott Williams & Wilkins, 2010, pp. 987-993.
  24. Pacheco, M.R., Baraldi-Artoni, S.M., & Ferreira, N. (2000)., Estudomorfométrico de hemácias de perdizes (Rhynchotusruflescens) mantidasemcativeiro., Braz.J. Morphol. Sci., 17,114.
  25. Pereira, M. E., Silveira, A. F. D., Guedes, A. G. P., & Silveira, S. O. (2003)., Hemocitometria em marreca do pé vermelho (Amazonetta brasiliensis)., Arq. ciênc. vet. zool. UNIPAR, 27-30.
  26. Arnall, L. & Keymer, I.F. (1975). Birddiseases. London: Baillière Tindall, pp 528., undefined, undefined
  27. Banks, W.J., & Sangue (1991)., Histologi aveterinária aplicada., In: Banks, W.J (ed). Manole. 2nd edn. São Paulo:, pp 87-203.
  28. Rupley, A.E. (1999)., Manual de clínicaaviária., São Paulo: Roca, pp 582.
  29. Fortes, E.A.M., Sousa, A.F., Almeida, E.C.S., Conde junior, A.M., & Moura, W.L. (2009)., Marphologiadascélulas do sangueperiférico em emas (Rhea americana)., Braz. J. Vet. Res. An. Sci., 46 (3), 215-221.
  30. Wakenell, P.S. (2010)., Hematology of Chickens and Turkeys., In: Weiss, D.J., & Wardrop, K.J. (eds). Schalm’s veterinary hematology, 6th edn. Ames: Lippincott Williams & Wilkins, pp 961.
  31. Campbell, T.W., Smith, S., & Zimmerman, K.l. (2010)., Hematology of waterfowl and raptors., In: Weiss, D.J., Wardrop, K.J. (eds). Schalm’s veterinary hematology, 6thedn., Ames: Lippincott Williams & Wilkins, pp 977.
  32. Harvey, J.W. (2001)., Atlas of Veterinary Hematology: Blood and Bone marrow of Domestic Animals., WB Saunders Company, USA, Philadelphia, pp 1-40.
  33. Narkkong, N.A., Aengwanich, W., & Tanomthong, A. (2011)., Morphology and morphometrics of hematological cells from eastern sarus crane., Grus Antigone sharpie. Comp. Clin. Pathol., 20(4), 299-304.
  34. Benez, S.M. (2001)., criação, clínica, teoria, prática., Silvestres, ornamentais, avinhados. 3rd ed. São Paulo: Robe, pp 522.
  35. Nirmalan, G.P., Atwal, O.S., & Carlson, H.C. (1972)., Ultrastructural studies on leukocytes and thrombocytes in circulating blood of japanese-quail., Poult. Sci., 51(6), 2050-2055. http://dx.doi.org/10.3382/ps.0512050.PMid: 4660986.
  36. Campbell, T.W. & Dein, F.J. (1984)., Avian hematology: the basics., Vet. Clin. North. Am. Small. Anim. Pract., 14(2), 223-248. http://dx.doi.org/10.1016/S0195-5616(84) 50031-X. PMid: 6610969.
  37. Jain, N.C., Feldman, B.F., & Zinkl, J.G. (2000)., Schalm’s veterinary hematology., 5th ed. Philadelphia: Lea & Febiger, pp 538.
  38. Coles, B.H., Krautwald-Junhanns, M.E. and Herrman, T.J. (1998)., Self-assessment picture tests: avian medicine., St. Louis: Mosby, pp 212.
  39. Morgulis, M.S. (2002)., Self-assessment icada., In: Macari M., Furlan RL, & Gonzales E (eds). Fisiologiaaviária aplicadaafrangosdecorte. Jaboticabal: Funep/Unesp, pp248-316.
  40. Gallo, S.S.M., Ederil, N.B., Bôa-Morte, M.O., & Oliveria, F.C.R. (2015)., Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilianbirds., Braz. J. Bio., http://dx.doi.org/ 10.1590/1519-6984.03414.