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Establishment of maternity by allelic comparison from the muscle tissue attached with femur bone of unidentified dead body

Author Affiliations

  • 1DNA Division, Regional Forensic Science Laboratory, Central Range, Mandi – 175002, Himachal Pradesh, India
  • 2Directorate of Forensics Services, Junga, Shimla – 171218, Himachal Pradesh, India
  • 3Department of Forensic Science, Punjabi University, Patiala – 147002, Punjab, India

Res. J. Forensic Sci., Volume 10, Issue (1), Pages 1-6, January,29 (2022)

Abstract

Femur bone is one of the common exhibit received in the forensic laboratories for DNA profiling from mass disasters, drowning, terrorist attacks, wars, explosion, forest fires, etc. In these cases, visual identification of deceased child’s is difficult. However, identification of such dead bodies is important for socio-legal purposes. Femur is largest bone of human thigh and DNA profile generated from this bone can play important role in the identification of victims. In this study, DNA profiles were generated from muscle tissue attached on femur bone of a deceased child and blood sample on FTA (Flinders Technology Associates) card of the putative mother. DNA from muscle tissue was isolated using magnetic bead based method with Qiagen EZ1 Advanced XL BioRobot, whereas, FTA card was purified using novel purification buffer. The DNA was subjected to Multiplex PCR amplification using GlobalFiler™ PCR Amplification Kit (ThermoFisher Scientific, U.S.A.). Capillary electrophoresis were done with 3500 XL Genetic Analyzer (Applied Biosystems, U.S.A.) and data were analyzed using GeneMapper™ ID-X Software v 1.6. The autosomal DNA analysis confirmed that deceased child was the biological son of putative mother.

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