International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Preventive Role of quercetin against Non-enzymatic Peroxidation and Oxidative stress in Brain Mitochondria induced by Endosulfan in Rat

    , , , , ,

Author Affiliations

  • 1Laboratory of Cellular and Molecular Biology, University of Jijelijel, ALGERIA
  • 2Department of animal biology, University of Mentouri 1, ALGERIA
  • 3Department of Biology, University of Tebessa, ALGERIA
  • 4Laboratory of Toxicology-Microbiology Environmental and Health, University of Sfax, TUNISIA

Int. Res. J. Earth Sci., Volume 3, Issue (1), Pages 18-23, January,25 (2015)

Abstract

Endosulfan (END) is still in use in north-Africa although it has large environmental ubiquity and toxicity.The non-enzymatic peroxidation and redox status in rat brain mitochondria after END toxicity have not been sufficiently investigated. In the present study, we first investigated the pro-oxidant effect of END on brain mitochondria and its effect upon lipid peroxidation in this organelle. Gavages of END into rats at a dose of 4 mg/kg induced oxidative stress in brain mitochondria, so it provoked a statistically significant reduction of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) levels and proteins. Significant increase in malonedialdehyde (MDA) levels – an indicator of lipid peroxidation – was observed in neuronal mitochondria. Second, the protective effect of quercetin (QE) (5mg/kg) against endosulfan-induced non-enzymatic peroxidation in the same organelle was also investigated. Indeed, the pretreatment of rats with QE protected brain mitochondria from oxidative stress and membrane modification. This treatment conserves the integrity of mitochondrial membranes following the inhibition of lipid peroxidation. Thus, QE works through the prevention of mitochondrial membrane perforation and the antioxidant defense system of brain mitochondria.

References

  1. World Health Organization (WHO)., The WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification 2000–2002. World Health Organization, International Program on Chemical Safety/Inter-Organization Program for the Sound Management of Chemicals, Geneva (2002)
  2. Silva M.H. and Gammon D., An assessment of the developmental, reproductive and neurotoxicity of endosulfan, Birth Defects Research Part B-Developmental and Reproductive Toxicology, 86(1),1-28 (2009)
  3. POPRC (Fourth meeting of the Persistent Organic Pollutants Review Committee), Geneva, Switzerland 13 - 17 October (2008)
  4. ATSDR (Agency for Toxic Substances and Disease Register). Toxicological Profile for Endosulfan, September 2000. Available at: http://www.atsdr.cdc.gov/toxprofiles /tp41.pdf. (2014)
  5. Sunitha S., Murthy V.K. and Mahmood R., Degradation of Endosulfan by Mixed Bacterial Cultures Enriched from Endosulfan Contaminated Soils of Southern India, International journal of bioscience, biochemistry and bio-informatics, 2(1), 31-35, DOI: 10.7763/IJBBB(2012)
  6. Zerouali E., Salghi R., Hormatallah Belkheir Hammouti A., Bazzi L. and Zaafarani Z., Pestide residues in tomatoes grown in greenhouse in Souss Massa Valley in Marocco and dissipation of endosulfan and Deltanethrin,. Fresenius Environmental Bulletin, 15(4), 267-271(2006)
  7. DeLorenzo M.E, Taylor L.A, Lund S.A, Pennington P.L., Strozier E.D. and Fulton M.H. Toxicity and Bioconcentration Potential of the Agricultural Pesticide: Endosulfan in Phytoplankton and Zooplankton, Arch Environ. Contam. Toxicol., 42, 173-181(2002)
  8. Hernandez F., Pitarch E., Serrano R., Gaspar J.V. and Olea N., Multiresidue determination of endosulfan and metabolic derivatives in human adipose tissue using automated liquid chromatographic cleanup and gas chromatographic analysis, Journal of Analytical Toxicology, 26(2), 94-103 (2002)
  9. Chan, M.P., Mohd, M.A. Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography–mass spectrometry, Environ. Toxicol.,20, 45–52(2005)
  10. Torres M.J., Folgoso C.C., Reche F.C., Velasco A.R. and Garcia I.C., Organochlorine pesticides in serum and adipose tissue of pregnant women in Southern Spain giving birth by cesarean section, Sci Total Environ372(1),32-8(2006)
  11. Cerrillo I., Granada A., Lopez-Espinosa M.J., Olmos B., Jimenez M. and Cano A. et al. Endosulfan and its metabolites infertile women, placenta, cord blood and human milk, Environ Res,98,233–9(2005)
  12. Durukan P., Ozdemir C., Coskun R., Ikizceli I., Esmaoglu A., Kurtoglu S. and Guven M., Experiences with endosulfan mass poisoning in rural areas, Eur J Emerg Med., 16(1), 53-6. doi: 10.1097/ MEJ.0b013e3283088e2b (2009)
  13. Silva M.H. and Beauvais S.L., Human health risk assessment of endosulfan. I: Toxicology and hazard identification, Regulatory Toxicology and Pharmacology, 56: p.4–17(2010)
  14. Paul V., Sheela S., Balasubramaniam E., Kazi M. Behavioural and biochemical changes produced by repeated oral administration of the insecticide endosulfan in immature rats, Indian J Physiol Pharmacol, 37, 204–8 (1993)
  15. Bebe F.N. and Panemangalore M., Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. J. Environ. Sci. Health B., 38,349-63 (2003)
  16. Valko M., Leibfritz D., Moncol J., Cronin M.T., Mazur M. and Telser J., Free radicals and antioxidants in normal physiological functions and human disease, Int. J. Biochem. Cell Biol.,39, 44-84(2007)
  17. Ng C.H., Mok, S.Z., Koh, C., Ouyang, X., Fivaz ML, Tan E.K., Dawson V.L., Dawson T.M., Yu F. and Lim K.L., Parkin protects against LRRK2 G2019S mutant-induced dopaminergic neurodegeneration in Drosophila, J. Neurosci. 29(36), 11257-11262 (2009)
  18. Halliwell B., Oxidative stress and neurodegeneration: where are we now?, Journal of Neurochemistry, 97, 1634–1658 (2006)
  19. Beal M.F., Aging, energy, and oxidative stress in neurodegenerative diseases. Ann Neurol, 38,357–366 (1995)
  20. Cassarino D.S. and Bennett J.P., An evaluation of the role of mitochondria in neurodegenerative diseases: mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration. Brain Research Reviews, 29(1), 1-25 (1999)
  21. Heijnen C.G., Haenen G.R. , Oostveen R.M., Stalpers E.M. and Bast A., Protection of flavonoids against lipid peroxidation : the structure activity relationship revisited, In Free Radical Research, 36, 575-581(2002)
  22. Kebieche M., Lakroun Z., Lahouel M., Bouayed J., Meraihi Z. and Souliman R., Evaluation of epirubicin-induced acute oxidative stress toxicity in rat liver cells and mitochondria, and the prevention of toxicity through quercetin administration, Experimental and Toxicologic Pathology, 61, 161–167 (2009)
  23. Formica J.V. and Regelson W., Review of the biology of Quercetin and related bioflavonoids, Food Chem Toxicol, 33 (12), 1061-1080 (1995)
  24. Utesch D., Feige K., Dasenbrock J., Broschard T.H., Harwood M., Danielewska-Nikiel B. and Lines T.C., Evaluation of the potential in vivo genotoxicity of quercetin, Mutation Research/Genetic Toxicology and EnvironmentalMutagenesis, 654, 38–44 (2008)
  25. Rustin P, Chre´ tien D, Bourgeron T, Ge´ rard B, Rotig A and Munnich A., Biochemical and molecular investigation in respiratory chain deficiencies, Clin Chem Acta,228, 35–51(1994)
  26. Lowry O.H., Rosegrough N.J., Farr A.L. and Randall R.J., Protein measurement with the folin phenol reagent, J Biol Chem.,193,265–75 (1951)
  27. Ohkawa H., Ohishi N. and Yagi K., Assay of lipid peroxides in animal tissue by thiobarbituric reaction, Anal Biochem., 95, 351–8 (1979)
  28. Ellman GL., Tissue sulfhidryl groups, Arch Biochem Biophys, 82, 70–7(1959)
  29. Clairborne A., Catalase activity. In: Greenwald RA, editor. CRC Handbook of Methods for Oxygen Radical Research. Boca Raton, FL: CRC Press, 283–4 (1985)
  30. Beauchamp C. and Fridovich I., Assay of superoxide dismutase, Anal Biochem, 44, 276–87 (1971)
  31. Ott M., Gogvadze V., Orrenius S. and Zhivotovsky B., Mitochondria, oxidative stress and cell death, Apoptosis, 12, 913–922 (2007)
  32. Di Monte D.A., Chan P. and Sandy M.S., Glutathione in Parkinson’s disease: a link between oxidative stress and mitochondrial damage?. Ann Neurol., 32, (S1) S111-S115 (1992)
  33. Sechi G., Deledda M. G., Bua G., Satta W.M., Deiana G.A., Pes G.M. and Rosati G., Reduced intravenous glutathione in the treatment of early Parkinson 's disease, Prog Neuropsychopharmacol Biol Psychiatry, 20(7), 1159-70 (1996)
  34. Kaur P., Radotra B., Minz R.W. and Gill K.D., Impaired mitochondrial energy metabolism and neuronal apoptotic cell death after chronic dichlorvos (OP) exposure in rat brain, Neurotoxicology, 28, 1208–1219 (2007)
  35. Ahmed T., Tripathi A.K., Ahmed R.S, Das S., Suke S.G., Pathak R., Chakraboti A. and Banerjee B.D., Endosulfan-induced apoptosis and glutathione depletion in human peripheral blood mononuclear cells: attenuation by N-acetylcysteine, J Biochem Mol Toxicol., 22, 299–304 (2008)
  36. Assefa Z., Van Laethem A., Garmyn M. and Agostinis P., Ultraviolet radiationinduced apoptosis in keratinocytes: on the role of cytosolic factors, Biochim Biophys Acta,1755, 90–106 (2005)
  37. Song Y., Liang X., Hu Y.,Wang Y., Yu H. And Yang K., p,p-DDE induces mitochondria mediated apoptosis of cultured rat Sertoli cells, Toxicology,253, 53–61(2008)
  38. Franco R., Sanchez-Olea R., Reyes-Reyes E.M. and Panayiotidis M.I., Environmental toxicity, oxidative stress and apoptosis: Menage a Trois. Mutat. Res.,674, 3–22 (2009)
  39. Merad-Boudia M., Nicole A., Santiard-Baron D., Saille C. and Ceballos-Picot I., Mitochondrial impairment as an early event in the process of apoptosis induced by glutathione depletion in neuronal cells: relevance to Parkinson 's disease . Biochem Pharmacol, 56(5), 645-55 (1998)
  40. Weber J., Halsall C.J., Muir D., Teixeira C., Small J., Solomon K., Hermanson M., Hung H. and Bidleman T., Endosulfan, a global pesticide: a review of its fate in the environment and occurrence in the Arctic, Sci. Total Environ.,408 (15), 2966-2984(2010)