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Isolation and characterisation of lumbrokinase from different species of earthworm

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Author Affiliations

  • 1Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637205, Tamilnadu, India
  • 2Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637205, Tamilnadu, India
  • 3Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637205, Tamilnadu, India
  • 4Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637205, Tamilnadu, India
  • 5Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637205, Tamilnadu, India

Res. J. Agriculture & Forestry Sci., Volume 8, Issue (3), Pages 57-60, July,8 (2020)

Abstract

Earthworms have been used in Chinese medicine for past few decades. Earthworm powder was widely used in traditional medicine. The research work is initiated by the study of fibrolytic property in earthworm powder. In traditional Chinese medicine, earthworm powder was used to treat thrombolytic disorder. After that the research work was carried out to know what is responsible for the anti-thrombotic property. Many research works were performed and finally isolate the enzyme responsible for that property and they also find that it is a fraction of protease enzyme .This fraction of enzyme was collectively known as lumbrokinase (LK). There are about 4000 species of earthworm are present in the world. Among them only few species are used as the composting worms. The widely used worms for vermicomposting are Eisenia fetida (red wrigglers), Eudrilus eugeniae (African night crawlers), etc. The intestinal tract of Earthworm was rich in enzymes; these enzymes have numerous therapeutic values. In order to find a drug from natural source; earthworm proteins were used as a source of drug. Lumbrokinase is a protease enzyme has fibrinolytic property so that it was widely used in treating myocardial infraction, thrombosis, DVT. Commercially lumbrokinase was known as Boluoke. Earthworm fibrinolytic enzyme (EFE) also known as Lumbrokinase (LK).

References

  1. Edy Parwanto, M.L., Mahyunis, Hardy Senjaya, Hosea Jaya and Syamsurizal (2016)., Fractionation and Characterization of Proteins in Lumbricus rubellus powders., International Journal of Pharmaceutical and Clinical Research, 8(1), 15-21.
  2. Zhenjum Sum (2015)., Earthworm as a biopharmaceutical: from traditional to precise., European Journal of BioMedical Research, 1(2), 28-35.
  3. Hwan cho, Eui sung choi, Hun Gil Lim and Hyung Hoan Lee (2004)., Purification and Characterization of six Fibrinolytic Serine-Proteases from Earthworm Lumbricus rubellus., Journal of Biochemistry and Molecular Biology, 37(2), 199-205
  4. Abdullah Adil Ansari and Kaminie Sitaram (2011)., An Investigation into the Anti-microbial and Anti-fungal Property of Earthworm powder obtained from Eisenia fetida., American Journal of Food Technology, 6(4), 329-335.
  5. Feng Wang, Chao Wang, Mei Li, Lulu Gui, Jiping Zhang and Wenrui Chang (2003)., Purification, characterization and crystallizations of a group of earthworm fibrinolytic enzymes from Eisenia fetida., Biotechnology Letters, 25(13), 1105-1109.
  6. Bhorgin Lourdumary and Murugaian, A.P.J (2014)., Fibrolytic Serine Protease isolated from Earthworm Lampito mauritii., International Journal of Science and Research, 3(10), 961-966.
  7. Phan, T. T. B., Ta, T. D., Nguyen, D. T. X., Van Den Broek, L. A., & Duong, G. T. H. (2011)., Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus., AMB express, 1(1), 26.
  8. Yan, X. M., Kim, C. H., Lee, C. K., Shin, J. S., Cho, I. H., & Sohn, U. D. (2010)., Intestinal absorption of fibrinolytic and proteolytic lumbrokinase extracted from earthworm, Eisenia andrei., The Korean Journal of Physiology & Pharmacology, 14(2), 71-75.
  9. Tang Mei, Liu Cao, Liang Zi, Gong Mingfu and Hu Die (2016)., Studies on Separation and Properties of Lumbrokinase in Pheretima praepinguis., Bangladesh Journal of Pharmacology, 45(1), 115-118.
  10. Edward K. Tillinghast, Ryan O’Donnell, Daniel Eves, Elizabeth Calvert and James Taylor (2001)., Water - Soluble Luminal contents of the gut of the earthworm Lumbricus terrestris L. and their Physiological significance., Comparative Biochemistry and Physiology, 129(2-3), 345-353.
  11. Cao Yong-Jun, Zhang Xia, Wang Wan-Hua, Zhai Wan-Quing, Quian Ju-Fen,Wang Jian-Sheng,Chen Jun, You Nian-Xing, Zhao Zhong, Wu Qui-Yi, Xu Yuan Lei, Li Rui-Xia and Liu Chun-Feng (2013)., Oral fibrinogen-depleting agent lumbrokinase for secondary ischemic stroke prevention: results from a multicenter, randomized, parallel-group and controlled clinical trial., Chinese Medical Journal, 126(21), 4060-4065.
  12. Ling, S. S., & Gurupackiam, M. (2017)., In-vitro evaluation of the antidiabetic & cytotoxic activities of earthworm (lumbricus rubellus) extracts., International Journal of Pharmaceutical Sciences and Research, 8(5), 2302-2308.
  13. Yong-Doo Park, Jong-Won Kim, Byong-Goo Min, Jeong-Won Seo, and Jong Moon Jeong (1998)., Rapid Purification and Biochemical Characteristics of Lumbrokinase III from Earthworm for use as a Fibrinolytic agent., Journal of Biotechnology Letters, 20(2), 169-172.
  14. Bhorgin Lourdumary, A.J., and Murugaian, P. (2012)., Fibrinolytic Serine Protease Isolated from Earthworm Lampito mauritii., International Journal of Science and Research, 3(10), 961-966.
  15. Prakash. M., Gunasekaran, G., and Elumalai, K. (2008)., Effect of earthworm powder on antioxidant enzymes in alcohol induced hepatotoxic rats., European Review for Medical and Pharmacological Sciences, 12, 237-243.
  16. Mingji Jin, Wei Chen, Wei Huang, Long Rong and Zhonggao Gao (2013)., Preparation of pegylated lumbrokinase and an evaluationof its thrombolytic activity both in vitro and in vivo., Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association, 3(2), 123-129.