Evaluation of mutagenic efficiency and effectiveness of gamma irradiation doses in two cultivars of bread wheat (Triticum aestivum L.)
- 1School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
- 2School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
- 3School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
- 4School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
Res. J. Agriculture & Forestry Sci., Volume 8, Issue (1), Pages 29-33, January,8 (2020)
Seed irradiation before sowing processes is one of the most efficient tools to improve the plant genetic diversity rapidly. The present research was carried out to evaluate the mutagenic efficiency and effectiveness of different gamma irradiation doses in bread wheat (Triticum aestivum L.). For this, healthy seeds of two varieties of bread wheat (Lok-1 and WH-147) were treated with various gamma irradiation doses (5, 10, 15, 20, 25, 30, 35, 40, 50kR) at Bhabha Atomic Research Center (BARC), Mumbai. The biological damage was evaluated in M1 populations by collecting the data related to plant lethality, seedling injury, meiotic aberrations and pollen sterility. Biological damage in M1 progenies and chlorophyll mutations in M2 populations were also estimated to measure the mutagenic efficiency and effectiveness of gamma rays. The results obtained showed that mutagenic effectiveness boosted along with increased dose of gamma irradiations whereas the efficiency of gamma ray treatments revealed irregular tendency depending on the biological parameter undertaken for its estimation. The lower as well as intermediate irradiation doses were successfully found to be much effective in producing populations with less genetic damage and with greater amount of macro-mutations. Among the bread wheat cultivars used, var. Lok-1 was found to be more sensitive to gamma-irradiations as compared to WH-147.
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