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Efficacy of chemical, botanical and biological for the management of Alternaria leaf spot disease of radish

Author Affiliations

  • 1Department of Plant Pathology, Agriculture and Forestry University, Rampur, Nepal
  • 2Nepal Polytechnique Institute, Purbanchal University, Bharatpur, Nepal
  • 3Department of Plant Pathology, Agriculture and Forestry University, Rampur, Nepal
  • 4Department of Plant Pathology, Agriculture and Forestry University, Rampur, Nepal

Res. J. Agriculture & Forestry Sci., Volume 6, Issue (8), Pages 1-6, August,8 (2018)


An experiment was conducted to evaluate the effect of different treatments viz. SAAF (mancozeb + carbendazim) (1%, 0.01% and 0.001%), Acorouscalamus root extract (1%, 0.5% and 0.25%) and Trichodermaharzianum extracted from local soil media and two radish varieties (Mino Early and Pyuthaney Rato) against Alternaria leaf spot disease (Alternariabrassicae) of radish in lab of Department of Plant Pathology AFU, Chitwan, Nepal during 2016. Poison food technique and dual culture technique was used to identify the efficacy of the treatments. The best check of mycelial growth of Alternariabrassicae was done by SAAF @ 0.1% which was followed by SAAF @ 0.01% and 0.001% respectively. Highest growth inhibition of the mycelium i.e. 93.65% was shown by SAAF @ 0.1% at the end of the investigation. The best control (31.94%) of the mycelium growth of the Alternariabrassicae was provided by Acorouscalamus root extract @ 1% on PDA which was statically at par with 0.5% of concentration inhibiting pathogen by 29.41%. Highest growth was observed in control. Result from the dual culture showed that at 4 days of incubation the Trichodermaharzianum. Completely covered the plate (8.9 cm) and Alternariabrassicae was limited within 2.30 cm diameter of colony size.


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