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Influence of meroterpene on economic characters of the shell ratio and denier scale in silkworm Bombyx mori (L.) (Race: PM x CSR2)

Author Affiliations

  • 1Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Malegaon (Baramati) Dist. Pune-413115, India
  • 2Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Malegaon (Baramati) Dist. Pune-413115, India
  • 3B.E. Society Daunds Lt. K. G. Kataria College of Arts & Science, Ambedkar Chawk, Siddhtech Road, Daund, District: Pune-413801, India

Res. J. Agriculture & Forestry Sci., Volume 5, Issue (10), Pages 7-15, October,8 (2017)

Abstract

Present study was undertaken to study the action of Meroterpene through the acetone solution on the economic characters shell ratio of cocoon and denier scale of silk filament in silk worm Bombyx mori (L.) through PM x CSR2 race. The meroterpene selected for the attempt was ferruginol. Three different concentrations of meroterpene - ferruginol compound used in the attempt include: 5ppm; 10ppm and 20 ppm. The larval stages of silkworm, Bombyx mori (L) utilized for the experimentation were the fifth instars. Five groups of larvae, each with hundred individuals were prepared for the study. The groups include: UT (Untreated Control group); STC (Solvent Treated Control group); T-1 (5ppm Meroterpene Treated Group); T-2 (10 ppm Meroterpene Treated Group) and T-3 (20 ppm Meroterpene Treated Group). Each group was consisted of hundred individuals. Separate micropipettes were used for topical application of ten microliteres of each concentration for respective groups. The meroterpene topical application was made to the individual larva of concerned group at 48 hours after the fourth moult. Standard schedule of rearing of the larval instars; provision of mountage to the mature larvae to spin their cocoons; harvesting the cocoons; reeling the cocoons for silk filament were followed. The shell ratio of the cocoons and the denier scale of silk filament from control group was measured 19.331 and 2.170 units respectively. Use of meroterpene ferruginol through acetone solvent for topical application to the larvae was found reflected into: significant improvement in the shell ratio of cocoons (from 21.407 to 22.948) and the silk filament denier scale (2.404 to 3.171). Meroterpene ferruginol at the rate at 5 ppm, 10 ppm and 20 ppm concentrations yielded the heaviest cocoon ( with weight: 2.046;2.387;2.924 gm) ; significant weight of cocoon - shell (0.438, 0.541, 0.673 gm), weight of pupa (1.613;1.846;2.252 gm). All three concentrations of meroterpene compound in the study recorded significant cocoon weight; Shell weight; pupal weight; shell ratio and denier scale in comparison with the control group. Specific titer of meroterpene ferruginol through solvent like acetone may lead to welcome new agro-biotechnological era for sericulture.

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