International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Altitudinal Variation of Bitter Principle of Swertia Chirayita and Its Standardization

    , , ,

Author Affiliations

  • 1Ministry of Forest and Soil Conservation, Singhdurbar, Kathmandu, Nepal
  • 2Chemistry Division, Forest Research Institute, Uttarakhand, Dehradun, India
  • 3Institute of Forestry, Pokhara Campus, Haryiokhara, Pokhara, Tribhuvan University, Nepal
  • 4Annapurna Research Center, Kathmandu, Nepal

Res. J. Agriculture & Forestry Sci., Volume 4, Issue (6), Pages 7-10, June,8 (2016)

Abstract

Swertia chirayita was obtained at an altitude of (1750-3250m) and three strata were distinguished on the basis of altitude. The bitterness values at strata 1 (1750-2250m), strata 2 (2251 – 2750m) and Strata 3(2751 – 3250m) was 1.33 %, 1.43% and 1.52% respectively; which shows slightly increasing trend with altitude and the average bitterness principle of the plant samples was 1.42 ± 0.06% . The parameters studied for ash standardization for total ash value, acid insoluble ash, and water soluble ash were 5.05± 0.08 %, 0.72± 0.06% and 0.84± 0.05%, respectively. Extract values; methanol extract 16.06 ± 0.41 % and water extract was 17.11± 2.33%. The thin layer chromatography used for the extraction of powder drug and the presence of bitter compounds was confirmed. This study provide the first data in standardization of Chirayita specifically in western Himalayas of Nepal and revealed the quality and purity of chirayita drugs setting down pharmacopoeia standards for future reference.

References

  1. Joshi K. and Li J., (2002)., Phylogenetics of Swertia L.(Gentianaceae-Swertiinae) and Molecular Differentiation of Swertia., 1-2.
  2. Joshi P. and Dhawan V., (2005)., Swertia chirayita – an overview., Current Science, 89(24), 635-640.
  3. Shrestha J.K., (2013)., Assessment of Genetic Diversity in Nepalese Populations of Swertia chirayita (Roxb. Ex Fleming) H. Karst Using RAPD-PCR Technique., American Journal of Plant Sciences, 4, 17-28.
  4. Anonymous. (1976)., The Wealth of India (Vols. X: Sp-w)., New Delhi: CSIR Publications.,77-82.
  5. Latif A. and Rehman S., (2014)., Standardization of A Herbal Medicine- Swertia Chirayita Linn., Pharmacophore, 5(1), 98-108.
  6. Sayyed M., Khan M., Devanna N., Syed Y.H., and Ansari J.A., (2013)., Pharmacognostical and phytochemical investigations of the whole plant of Swertia chirata and Hemidesmus indicus., Journal of Pharmaceutical and Biosciences, (4), 141-145.
  7. Butt B., Srivastva L. and Chand R., (1999)., Chemical Screening of Chirayita (Swertia chirayita) Karst Collections from Himachal Pradesh for Bitter Content Variability., Ancient Science of Life, 18(3 & 4), 1-4.
  8. WHO (2011)., Quality Control Methods for Herbal Materials., Malta: WHO 23-31.
  9. Htttp.www.googleearth.com/March/20013, undefined, undefined
  10. Conservation and Management Committee. (2010)., Management Operation Plan. Bhujung U.C.O: Annapurna Conservation Area Project., 4-56.
  11. Anonymous. (1986)., The Ayurvedic Pharmacoepia of India India:Department of Ayush., I. Ministry of Family and Health and Family welfare (1 Edition., Vol. 1 (1) 98-100.
  12. Siddha C.C. (2007)., HPTLC- Fingerprint atlas of Ayurvedic Single Plant Drugs Mentioned in Ayurvedic Pharmacopeia., Volume 1. New Delhi, India: Department of Ayush 1-5.
  13. Wagner H. and Bladt S., (1995)., Plant Drug Analysis., Munchen, Germany: University of Munchen 73-75.