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Altitudinal Variation of Bitter Principle of Swertia Chirayita and Its Standardization

Author Affiliations

  • 1Ministry of Forest and Soil Conservation, Singhdurbar, Kathmandu, Nepal
  • 2Chemistry Division, Forest Research Institute, Uttarakhand, Dehradun, India
  • 3Institute of Forestry, Pokhara Campus, Haryiokhara, Pokhara, Tribhuvan University, Nepal
  • 4Annapurna Research Center, Kathmandu, Nepal

Res. J. Agriculture & Forestry Sci., Volume 4, Issue (6), Pages 7-10, June,8 (2016)


Swertia chirayita was obtained at an altitude of (1750-3250m) and three strata were distinguished on the basis of altitude. The bitterness values at strata 1 (1750-2250m), strata 2 (2251 2750m) and Strata 3(2751 3250m) was 1.33 %, 1.43% and 1.52% respectively; which shows slightly increasing trend with altitude and the average bitterness principle of the plant samples was 1.42 0.06% . The parameters studied for ash standardization for total ash value, acid insoluble ash, and water soluble ash were 5.05 0.08 %, 0.72 0.06% and 0.84 0.05%, respectively. Extract values; methanol extract 16.06 0.41 % and water extract was 17.11 2.33%. The thin layer chromatography used for the extraction of powder drug and the presence of bitter compounds was confirmed. This study provide the first data in standardization of Chirayita specifically in western Himalayas of Nepal and revealed the quality and purity of chirayita drugs setting down pharmacopoeia standards for future reference.


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