International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Influence of Streptomyces fradiae against Root knot nematode Meloidogyne incognita in Tomato

Author Affiliations

  • 1Department of Nematology, Tamil Nadu Agricultural University, Coimbatore -641003, Tamil Nadu, INDIA
  • 2Department of Nematology, Tamil Nadu Agricultural University, Coimbatore -641003, Tamil Nadu, INDIA

Res. J. Agriculture & Forestry Sci., Volume 3, Issue (1), Pages 6-11, January,8 (2015)


A protected and field experiment was conducted to evaluate the biocontrol potential of the optimized culture filtrates of Streptomyces fradiae against root-knot nematode Meloidogyne incognita in tomato. The fermentation medium of S. fradiae optimized with pH 6 at 35oC with starch and yeast extract as carbon and nitrogen sources respectively maximized the colonization rate of S. fradiae. The culture filtrate of the optimized medium of S. fradiae resulted in higher degree of inhibition in egg hatching, and mortality of juveniles of M. incognita. The effectiveness of optimized medium of S. fradiae against M .incognita is related to higher production of secondary metabolites subsequent to maximization of colonization. The S. fradiae experimented for the management of M. incognita under protected and field conditions revealed its effectiveness for the suppression of M .incognita population and to reduce the severity of nematode disease in terms of gall index. The suppression in M. incognita population / incidence following the application of S. fradiae resulted in significant improvement in yield attributes including fruit yield of tomato.


  1. Barker K.R., Introduction and synopsis of advancements in nematology, In : Barker, K.R., Pederson, G.A., Windham, G.L. (Eds.), Plant and Nematode Interactions. Am. Soc.of Agronomy, Inc., Crop Sci. Am. Inc., and Soil Sci. Soc. Am. Inc., Madison, WI, 120 (1998)
  2. Mohsin M., Influence of inoculum level on pathogenicity and population development of noot-knot nematode on potato and susceptibility of its clones to the nematode, M.Sc. (Ag) thesis submitted to the Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 59, (1987)
  3. Hay F.S. and Skipp R.A., Fungi and actinomycetes associated with cysts of Heterodera trifolii Goffart (nematoda: Tylenchida) in pasture soils in New Zealand, Nematologica, 39, 376384 (1993)
  4. Dicklow M.B., Acosta N. and Zuckerman B.M., A novel Streptomyces species for controlling plant-parasitic nematodes, J. Chem. Ecol., 19, 159173 (1993)
  5. Challis G.L. and Hopwood D.A., Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species, Proc. Natl. Acad. Sci., 100, 14555-14561 (2003)
  6. Jansson R.K. and Rabatin S., Potential of foliar, dip and injection applications of avermectins for control of plant parasitic nematodes, J. Nematol., 30, 65-75 (1998)
  7. Krechel A., Faupel A, Hallman J, Ulrich A. and Berg. G., Potato-associated bacteria and their antagonistic potential toward plant pathogenic fungi and the plant parasitc nematode Meloidogyne incognita (Kofoid and White) Chitwood, Can.J. Microbiol., 48, 772-786 (2002)
  8. Man-Hong Sun, Li Gao, Yan-Xia Shi, Bao-Ju Li and Xing-Zhong Liu., Fungi and actinomycetes associated with Meloidogyne spp, Eggs and females in China and their biocontrol potential, Journal of Invertebrate Pathology, 93, 2228 (2006)
  9. Ellaiah P., Srinivasulu B. and Adinarayana. K., Optimisation studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation process, Biochem., 39, 529-534 (2004)
  10. Agenes E.A., Abiodun I.S. and Olussola B.O., Solid state fermentation production of tetracycline by Streptomyces strains using some agricultural weast as substrate, World J. Microbiol. Biotech., 21, 107-114 (2005)
  11. Yang S.S. and Yuan S.S., Oxytetracycline production by Streptomyces rimosus in solid state fermentation of sweet potato residue, World J. Micro. Biotechnol., 6, 244-236 (1990)
  12. Vidhyasekaran P. and Muthuamalin M, Evaluation of powder formulation of P. fluorescens Pf1 for control of rice sheath blight, Biocontrol Sci. Techn., 9, 67-74 (1999)
  13. Pornthip Ruanpanun, Hartmut Laatsch, Nuchanart Tangchitsomkid and Saisamorn Lumyong, Nematicidal activity of fervenulin isolated from a nematicidal actinomycete, Streptomyces sp. CMU-MH021, on Meloidogyne incognita, World J Microbiol Biotechnol., 27, 13731380 (2011)
  14. Jayakumar J., Rajendran G. and Ramakrishnan S., Screening of Streptomyces avermitilis culture filtrate in vitro against root knot and reniform nematodes, Indian J. Pl. Protec., 33, 129-133 (2005)
  15. Faske T.R. and Starr J.L., Sensitivity of Meloidogyne incognita and Rotylenchulus reniformisto abamectin, Journal of Nematology, 38, 240244 (2006)
  16. Subhashini D.V. and Padmaja K., Effect of fungicides and native antagonist against Pythium aphanidermatum in tobacco Nurseries, Ann. Pl. Protec. Sci., 19, 392-395 (2011)
  17. Aldesuquy H.S., Mansour F.A. and Abo-Hamed S.A., Effect of the culture filtrates of Streptomyces on growth and productivity of wheat plants, Folia Microbiol, 43, 465-470 (1998)
  18. Glick B.R.,, The enhancement of plant growth by freeliving bacteria, Can., J. Nematol., 9, 71-79 (1995)