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Effect of Wounding and Plant Growth Regulators (IBA and NAA) on root proliferation of Taxus wallichiana shoot cuttings

Author Affiliations

  • 1Department of Environmental Studies, North-Eastern Hill University, Shillong-793022, Meghalaya, INDIA

Res. J. Agriculture & Forestry Sci., Volume 2, Issue (12), Pages 8-14, December,8 (2014)


Meghalaya, a hilly state of Northeast India is blessed with a few natural stocks of Taxus wallichiana, a highly valued medicinal tree. However its habitat destruction due to several reasons posed a serious threat to its existence in the region. Therefore, ex-situ conservation measures are urgently needed to prevent this valuable resource from perishing. In order to optimize the propagation protocol of Taxus wallichiana by shoot cuttings in subtropical climate of Northeast India, the present study was carried out to investigate the effect of shoot type (softwood, semi-hardwood and hardwood) wounding (light and severe), plant growth regulators (IBA and NAA) and their interactive effect on adventitious rooting. Semi-hardwood (29.7%) and hardwood cuttings (26.1%) had higher survival percentage than softwood (5.6%). Overall better rooting response was exhibited by semi-hardwood cutting having 43.9 % rooted cutting, 15.9 roots per cutting and 6 cm of average root length. The poor rooting was observed in softwood cutting. Severe wounded cuttings initiated root development earlier than light wounded (76.9 days vs. 87.0 days). In addition, severe wounding also increased root production (10.5) in shoot cuttings having maximum root number per cutting with semi-hardwood (19.1). Among plant growth regulators, IBA treated cuttings exhibited overall better rooting response. In contrast, application of NAA proved phyto-toxic to plant as all of them died due to basal necrosis. For, softwood and semi hardwood higher rooting success was achieved with 6.15 mM IBA and for hardwood with higher concentration of 24.6 mM IBA.


  1. Lanker U., Malik A.R., Gupta N.K. and Butola J.S., Natural regeneration status of the endangered medicinal plant Taxus baccata Hook.F. Syn. T. Wallichiana, in northwest Himalaya, International Journal of Biodiversity Science, Ecosystem Services & Management 6(1-2), 20-27 (2010)
  2. Bhatnagar S.P. and Moitra A., Gymnosperms, New Age International Ltd, New Delhi, 310 (1996)
  3. Jaziri M., Zhiri A., Guo Y.W., Dupont J.P. and Shimomura K, Taxus sp. Cell tissue and organ culture as alternatives sources of taxoid production: A literature survey, Plant cell tiss org., 46, 59-75 (1996)
  4. Pant S. and Samant S., Population Ecology of The Endangered Himalayan Yew in Khokan Wildlife Sanctuary of North Western Himalaya, J. Mt. Sci ., 5, 257-264 (2008)
  5. Steinfield D., Early lessons from propagating Pacific yew, Rocky Mountain Forest and Range Experiment Station Technical Research Report, 221 (1992)
  6. Zarek M., A practical method for overcoming the dormancy of Taxus baccata isolated embryos under in vitro conditions, In Vitro Cell Dev Bio—Plant,43, 623–630 (2007)
  7. Flores H. E., and Scrignoli P.J., In vitro culture precocious germination of Taxus embryos, In Vitro Cell Dev Bio—Plant, 27, 139–142 (1991)
  8. Rajewski M., Lange S. and Hattemer H.H., Problems of reproduction in the genetic conservation of rare tree species: the example of common yew (Taxus baccata L.), Forest Snow Landscape Research, 75, 251–266 (2000)
  9. Schneck V., Studies on influence of clone on rooting ability and rooting quality in the propagation of cuttings from 40 to 350-year-old Taxus baccata L. ortets, Silvae Genet., 45, 246–249 (1996)
  10. Khali R.P. and Sharma A.K., Effect of phytohormones on propagation of Himalayan Yew (Taxus accata L.) through stem cuttings, Indian Forester, 129, 289-294(2003)
  11. Metaxes J.D., Thomas D.S., Yupsani T. and Economou A.S., Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment. Plant Growth Regulation 44, 257-266 (2004)
  12. Fordham A.J. and Spraker L.S., Propagation manual of selected gymnosperms, Arnoldia, 37, 1-88 (1997)
  13. Ragonezi C., Klimaszewska K., Castro R.M., Lima M., Oliveira P de. and Zavattieri M. A., Adventitious rooting of conifers: influence of physical and chemical factors (Review), Tree 24, 975-992 (2010)
  14. Mitter H. and Sharma A., Propagation of Taxus baccata Linn. by stem cutting , Indian Forester, 125 (2), 289-294 (1999)
  15. Nandi S.K., Palni L.M.S. and Rikhari H.C., Chemical induction of adventitious root formation in Taxus baccata cuttings, Plant Growth Regulation, 19, 117- 122 (1996)
  16. Kaul K., Variation in rooting behaviour of stem cuttings in relation to their origin in Taxus wallichiana Zucc., New Forests, 36, 217-224 (2008)
  17. Kayang H., Kharbuli B. and Syiem D., Role of community in biodiversity conservation: a case study in Khasi hills of Meghalaya, India, Journal of Basic and Applied Biology, 1, 1-5 (2007)
  18. Mao A.A., Hynniewta T.M. and Sanjappa M., Plant wealth of Northeast India with reference to ethonobotany, Indian Journal of Traditional Knowledge, 8(1), 96-103 (2009)
  19. Hartmann H.T., Kester D.E., Geneve R.L. and Davies F.T., Plant propagation principle and practices. Prentice- Hall of India Private Limited, New Delhi, 277-403, (2009)
  20. Hoshmand A.R., Experimental Research Design and Analysis, CRC Press, Inc., United State of America (1994)
  21. Dirr M.A. and Heuser C.W, The reference manual of woody plant propagation: from seed to tissue culture, Varsity press, Inc., Georgia, 23 (1987)
  22. Wells J.S., Wounding cutting as a commercial practice. Comb. Proc. Intl. Plant Prop. Soc., 12, 47-55 (1962)
  23. Mateo-Sa´nchez J.J., Vargas-Herna´ndez J.J., Lo´pez- Peralta M.C., Jasso-Mata J., Rooting of juvenile cuttings of five species of ornamental conifer : Effect of indolebutyric acid and temperature, Ciencia Forestal en Mexico, 23(84), 29-38 (1998)
  24. Trewavas A.J. and Cleland R.E., Is plant development regulated by changes in concentration of growth substance or by changes in the sensitivity to growth substance?, Trends Biochem Sci., 8, 354-357 (1983)