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Effect of Wounding and Plant Growth Regulators (IBA and NAA) on root proliferation of Taxus wallichiana shoot cuttings

Author Affiliations

  • 1Department of Environmental Studies, North-Eastern Hill University, Shillong-793022, Meghalaya, INDIA

Res. J. Agriculture & Forestry Sci., Volume 2, Issue (12), Pages 8-14, December,8 (2014)

Abstract

Meghalaya, a hilly state of Northeast India is blessed with a few natural stocks of Taxus wallichiana, a highly valued medicinal tree. However its habitat destruction due to several reasons posed a serious threat to its existence in the region. Therefore, ex-situ conservation measures are urgently needed to prevent this valuable resource from perishing. In order to optimize the propagation protocol of Taxus wallichiana by shoot cuttings in subtropical climate of Northeast India, the present study was carried out to investigate the effect of shoot type (softwood, semi-hardwood and hardwood) wounding (light and severe), plant growth regulators (IBA and NAA) and their interactive effect on adventitious rooting. Semi-hardwood (29.7%) and hardwood cuttings (26.1%) had higher survival percentage than softwood (5.6%). Overall better rooting response was exhibited by semi-hardwood cutting having 43.9 % rooted cutting, 15.9 roots per cutting and 6 cm of average root length. The poor rooting was observed in softwood cutting. Severe wounded cuttings initiated root development earlier than light wounded (76.9 days vs. 87.0 days). In addition, severe wounding also increased root production (10.5) in shoot cuttings having maximum root number per cutting with semi-hardwood (19.1). Among plant growth regulators, IBA treated cuttings exhibited overall better rooting response. In contrast, application of NAA proved phyto-toxic to plant as all of them died due to basal necrosis. For, softwood and semi hardwood higher rooting success was achieved with 6.15 mM IBA and for hardwood with higher concentration of 24.6 mM IBA.

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